Cissus quadrangularis plant extract enhances the development of cortical bone and trabeculae in the fetal femur

M.S. Rao, P. Bhagath Kumar, V.B. Narayana Swamy, N. Gopalan Kutty

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Traditional recipes for treatment of physical and mental ailments exist in all major ancient civilizations of the world. One such recipe popular in the Indian subcontinent involves the use of the extract of Cissus quadrangularis plant. Cissus quadrangularis is a shrub, with thick and fleshy quadrangular stem. The roots and stem are most useful for healing of fracture of the bones. The question whether the plant Cissus quadrangularis, which is proved to contain phyto-estrogens will increase the bone mass during fetal growth is not addressed. Accordingly the objective of the present study was to evaluate the effect of alcoholic extract of Cissus quadrangularis on the intrauterine growth of trabeculae in the long bones in rats. Pregnant rats were administered with Ethanol extract of Cissus quadrangularis (CQ), orally, at the dose of 750, mg/kg body weight from 9th day of gestation till delivery. Femur bone of the newborn pups were collected, decalcified and processed for paraffin sectioning. Thickness of cortical bone at mid shaft level, thickness of individual trabeculae were measured using a calibrated ocular micrometer. Results showed a significantly increase in thickness of the cortical bone at mid shaft level compared to control rats. The thickness of individual trabecula was increased significantly. The present study demonstrates enhanced bone formation during fetal growth by CQ, which may be related to rich content of calcium, phosphorus and phytoestrogenic property of the plant.
Original languageEnglish
Pages (from-to)190-202
Number of pages13
JournalPharmacologyonline
Volume3
Publication statusPublished - 2007

Fingerprint

Cissus
Plant Extracts
Femur
Fetal Development
Bone and Bones
Civilization
Phytoestrogens
Fracture Healing
Bone Fractures
Osteogenesis
Paraffin
Phosphorus
Cortical Bone
Ethanol
Body Weight
Newborn Infant
Calcium
Pregnancy
Growth

Cite this

Rao, M. S., Bhagath Kumar, P., Narayana Swamy, V. B., & Gopalan Kutty, N. (2007). Cissus quadrangularis plant extract enhances the development of cortical bone and trabeculae in the fetal femur. Pharmacologyonline, 3, 190-202.
Rao, M.S. ; Bhagath Kumar, P. ; Narayana Swamy, V.B. ; Gopalan Kutty, N. / Cissus quadrangularis plant extract enhances the development of cortical bone and trabeculae in the fetal femur. In: Pharmacologyonline. 2007 ; Vol. 3. pp. 190-202.
@article{230c5825cd8b4e0db9d968f118034ec5,
title = "Cissus quadrangularis plant extract enhances the development of cortical bone and trabeculae in the fetal femur",
abstract = "Traditional recipes for treatment of physical and mental ailments exist in all major ancient civilizations of the world. One such recipe popular in the Indian subcontinent involves the use of the extract of Cissus quadrangularis plant. Cissus quadrangularis is a shrub, with thick and fleshy quadrangular stem. The roots and stem are most useful for healing of fracture of the bones. The question whether the plant Cissus quadrangularis, which is proved to contain phyto-estrogens will increase the bone mass during fetal growth is not addressed. Accordingly the objective of the present study was to evaluate the effect of alcoholic extract of Cissus quadrangularis on the intrauterine growth of trabeculae in the long bones in rats. Pregnant rats were administered with Ethanol extract of Cissus quadrangularis (CQ), orally, at the dose of 750, mg/kg body weight from 9th day of gestation till delivery. Femur bone of the newborn pups were collected, decalcified and processed for paraffin sectioning. Thickness of cortical bone at mid shaft level, thickness of individual trabeculae were measured using a calibrated ocular micrometer. Results showed a significantly increase in thickness of the cortical bone at mid shaft level compared to control rats. The thickness of individual trabecula was increased significantly. The present study demonstrates enhanced bone formation during fetal growth by CQ, which may be related to rich content of calcium, phosphorus and phytoestrogenic property of the plant.",
author = "M.S. Rao and {Bhagath Kumar}, P. and {Narayana Swamy}, V.B. and {Gopalan Kutty}, N.",
note = "Cited By :12 Export Date: 10 November 2017 Correspondence Address: Rao, M.S.; Department of Anatomy, KMC, Manipal University, Manipal 576104, India; email: muddannas@yahoo.com Chemicals/CAS: calcium, 7440-70-2; phosphorus, 7723-14-0 References: Udupa, K.N., Chaturvedi, G.N., Tripathi, S.N., (1970) Advances in research in Indian medicine, 12, pp. 165-196. , Banaras Hindu University, Varanasi,India; Kumbhojkar, M.S., Kulkarni, D.K., Upadhye, A.S., Ethno botany of Cissus quadrangularis L. from India (1991) Ethnobotany, 3, pp. 21-25; Murthy, K.N.C., Vanitha, A., Swami, M.M., Ravi shankar, G., Antioxidant and antimicrobial activity of Cissus quadrangularis L (2003) J. Med.Food, 6, pp. 99-105; Adesanya, Saburi, A., Rene, N., Stilbene derivatives from Cissus quadrangularis (1999) J.Nat. Prod, 62, pp. 1694-1695; Enechi, O.C., Odonwodo, I., (2003) An assessment of the phytochemical and nutrient composition of the pulverized root of Cissus quadrangularis, 1, pp. 63-68. , Bio Research; Yoganarisimhan, S.N., (2000) Medicinal Plants of India, 2, pp. 136-137. , Cyber Media Bangalore; Jayaweera DMA. Medicinal plants used in Ceylon (Parts 01-05). The national Science Council of Srilanka 1980-1882; Deka, D.K., Lahon, L.C., Saikia, J., Mukit, A., Effect of Cissus quadrangularis in accelerating healing process of experimentally fractured Radius-Ulna of dog: A preliminary study (1994) Indian Journal of Pharmacology, 26, pp. 44-45; Nadakarni, A.K., (1954) Indian Meteria Medica, pp. 284-285. , 13th ed; Udupa, K.N., Prasad, G.C., Sen, S.P., The effect of phytogenic steroid in the acceleration of fracture repair (1965) Life Science, 4, p. 317; Udupa, K.N., Prasad, G., 10 further studies on the effect of Cissus quadrangularis in accelerating fracture healing (1964) Indian Journal of Medical Research, 52, p. 26; Chopra, S.S., Patel, M.R., Awadhiya, R.P., Studies on Cissus quadrangularis in experimental fracture repair: A histopathological study (1976) Indian Journal of Medical Research, 64, pp. 1365-1368; Sen, S.P., Studies on the active constituents of Cissus quadrangularis wall (1966) Current Science, 35, p. 317; Prasad, G.C., Udupa, K.N., Effect of Cissus quadrangularis on the healing of cortisone treated fracture (1963) Indian Journal of Medical Research, 51, p. 667; Prasad, G.C., Udupa, K.N., Pathways and site of a phytogenic steroid from Cissus quadrangularis (1972) Journal of Research in Indian Medicine, 4, p. 132; Wronsky, T.J., Cintron, M., Doherty, A.L., Dann, L.M., Estrogen treatment prevents osteopenia and depresses bone turnover in ovariectomized rats (1988) Endocrinology, 123, pp. 681-686; Takano-Yamamoto, T., Rodan, G.A., Direct effects of 17β-estradiol on trabecular bone in ovariectomized rats (1990) Proc Natl Acad Sci. USA, 87, pp. 2172-2176; Turner, R.T., Vandersteenhoven, J.J., Bell, N.H., The effects of ovariectomy and 17β-estrdiol on cortical bone histomorphometry in growing rats (1987) J Bone Miner Res, 2, pp. 115-122; Turner, R.T., Riggs, B.L., Spelsberg, T.C., Skeletal effects of estrogen (1994) Endocr Rev, 15, pp. 275-308; Komm, B.S., Terpening, C.M., Benz, D.J., Graeme, K.A., Gallegos, A., Estrogen binding, receptor mRNA and biologic response in osteoblast-like osteosarcoma cells (1988) Science, 241, pp. 81-84; Eriksen, E.F., Colvard, D.S., Berg, N.J., Graham, M.L., Mann, K.G., Spelberg, T.C., Riggs, B.L., Evidence of estrogen receptor in normal human osteoblast like cells (1988) Science, 241, pp. 84-86; Oursler, T.V.I.J., Osdoby, P., Pyfferon, J., Riggs, B.L., Spelsberg, T.C., Avain osteoclasts as estrogen target cells (1991) Proc Natl Acad Sci USA, 88, pp. 6613-6617; Gray, T.K., Flynn, T.C., Gray, K.M., Nabell, L.M., 17β estrodiol acts directly on the clonal osteoblastic cell line UMR-106 (1987) Proc Natl acad sci USA, 84, pp. 6267-6271; Ernst, M., Parker, M.G., Rodan, G.A., Functional estrogen receptor in osteoblastic cells demonstrated by transfection with a receptor gene containing an estrogen response element (1991) Mol endocrinol, 5, pp. 1597-1606; Migliaccio, S., Davis, V.L., Gibson, M.K., Gray, K.T., Korach, K.S., Estrogens modulate the responsiveness of osteoblast like cells (ROS 17/2.8) stably transfected with estrogen receptor (1992) Endocrinology, 130, pp. 2617-2624; Brandi ML. Flavanoids: Biochemical effects and therapeutic applications. Bone Miner, 1992; 19(Suppli 1):S3-14; Lindsay, R., Hart, D.M., Aikken, J.M., McDonald, E.B., Anderson, J.B., Clark, A.C., Long-term prevention of postmenopausal osteoporosis by estrogen: Evidence of an increased bone mass after delayed onset of estrogen treatment (1976) Lancet, 1, pp. 1038-1040; Greenman, D.I., Delongchamp, R.R., Interactive response to DES in CH3 mice (1986) Food Chem Toxicol, 24, pp. 931-934; Yasuo U, Taguchi O,Noumura T,Iguchi T. Effect of sex steroids on the development of sexual dimorphism in mouse innominate bone. 1992, Anat rec.234:541-548; Migliaccio, S., Newbold, R.R., Bullock, B.C., Jefferson, W.J., Sutton Jr, F.G., Mclachlan, J.A., Korach, K.S., Alterations of maternal estrogen levels during gestation affect the skeleton of -female offspring (1996) Endocninology, 137, pp. 2118-2125; Sanyal, A., Ahmad, A., Sastry, M., Calcite growth in Cissus quadrangularis plant extract, a traditional Indian bone healing aid (2005) Current science, 10, pp. 1742-1745",
year = "2007",
language = "English",
volume = "3",
pages = "190--202",
journal = "Pharmacologyonline",
issn = "1827-8620",
publisher = "SILAE (Italo-Latin American Society of Ethnomedicine)",

}

Rao, MS, Bhagath Kumar, P, Narayana Swamy, VB & Gopalan Kutty, N 2007, 'Cissus quadrangularis plant extract enhances the development of cortical bone and trabeculae in the fetal femur', Pharmacologyonline, vol. 3, pp. 190-202.

Cissus quadrangularis plant extract enhances the development of cortical bone and trabeculae in the fetal femur. / Rao, M.S.; Bhagath Kumar, P.; Narayana Swamy, V.B.; Gopalan Kutty, N.

In: Pharmacologyonline, Vol. 3, 2007, p. 190-202.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cissus quadrangularis plant extract enhances the development of cortical bone and trabeculae in the fetal femur

AU - Rao, M.S.

AU - Bhagath Kumar, P.

AU - Narayana Swamy, V.B.

AU - Gopalan Kutty, N.

N1 - Cited By :12 Export Date: 10 November 2017 Correspondence Address: Rao, M.S.; Department of Anatomy, KMC, Manipal University, Manipal 576104, India; email: muddannas@yahoo.com Chemicals/CAS: calcium, 7440-70-2; phosphorus, 7723-14-0 References: Udupa, K.N., Chaturvedi, G.N., Tripathi, S.N., (1970) Advances in research in Indian medicine, 12, pp. 165-196. , Banaras Hindu University, Varanasi,India; Kumbhojkar, M.S., Kulkarni, D.K., Upadhye, A.S., Ethno botany of Cissus quadrangularis L. from India (1991) Ethnobotany, 3, pp. 21-25; Murthy, K.N.C., Vanitha, A., Swami, M.M., Ravi shankar, G., Antioxidant and antimicrobial activity of Cissus quadrangularis L (2003) J. Med.Food, 6, pp. 99-105; Adesanya, Saburi, A., Rene, N., Stilbene derivatives from Cissus quadrangularis (1999) J.Nat. Prod, 62, pp. 1694-1695; Enechi, O.C., Odonwodo, I., (2003) An assessment of the phytochemical and nutrient composition of the pulverized root of Cissus quadrangularis, 1, pp. 63-68. , Bio Research; Yoganarisimhan, S.N., (2000) Medicinal Plants of India, 2, pp. 136-137. , Cyber Media Bangalore; Jayaweera DMA. Medicinal plants used in Ceylon (Parts 01-05). The national Science Council of Srilanka 1980-1882; Deka, D.K., Lahon, L.C., Saikia, J., Mukit, A., Effect of Cissus quadrangularis in accelerating healing process of experimentally fractured Radius-Ulna of dog: A preliminary study (1994) Indian Journal of Pharmacology, 26, pp. 44-45; Nadakarni, A.K., (1954) Indian Meteria Medica, pp. 284-285. , 13th ed; Udupa, K.N., Prasad, G.C., Sen, S.P., The effect of phytogenic steroid in the acceleration of fracture repair (1965) Life Science, 4, p. 317; Udupa, K.N., Prasad, G., 10 further studies on the effect of Cissus quadrangularis in accelerating fracture healing (1964) Indian Journal of Medical Research, 52, p. 26; Chopra, S.S., Patel, M.R., Awadhiya, R.P., Studies on Cissus quadrangularis in experimental fracture repair: A histopathological study (1976) Indian Journal of Medical Research, 64, pp. 1365-1368; Sen, S.P., Studies on the active constituents of Cissus quadrangularis wall (1966) Current Science, 35, p. 317; Prasad, G.C., Udupa, K.N., Effect of Cissus quadrangularis on the healing of cortisone treated fracture (1963) Indian Journal of Medical Research, 51, p. 667; Prasad, G.C., Udupa, K.N., Pathways and site of a phytogenic steroid from Cissus quadrangularis (1972) Journal of Research in Indian Medicine, 4, p. 132; Wronsky, T.J., Cintron, M., Doherty, A.L., Dann, L.M., Estrogen treatment prevents osteopenia and depresses bone turnover in ovariectomized rats (1988) Endocrinology, 123, pp. 681-686; Takano-Yamamoto, T., Rodan, G.A., Direct effects of 17β-estradiol on trabecular bone in ovariectomized rats (1990) Proc Natl Acad Sci. USA, 87, pp. 2172-2176; Turner, R.T., Vandersteenhoven, J.J., Bell, N.H., The effects of ovariectomy and 17β-estrdiol on cortical bone histomorphometry in growing rats (1987) J Bone Miner Res, 2, pp. 115-122; Turner, R.T., Riggs, B.L., Spelsberg, T.C., Skeletal effects of estrogen (1994) Endocr Rev, 15, pp. 275-308; Komm, B.S., Terpening, C.M., Benz, D.J., Graeme, K.A., Gallegos, A., Estrogen binding, receptor mRNA and biologic response in osteoblast-like osteosarcoma cells (1988) Science, 241, pp. 81-84; Eriksen, E.F., Colvard, D.S., Berg, N.J., Graham, M.L., Mann, K.G., Spelberg, T.C., Riggs, B.L., Evidence of estrogen receptor in normal human osteoblast like cells (1988) Science, 241, pp. 84-86; Oursler, T.V.I.J., Osdoby, P., Pyfferon, J., Riggs, B.L., Spelsberg, T.C., Avain osteoclasts as estrogen target cells (1991) Proc Natl Acad Sci USA, 88, pp. 6613-6617; Gray, T.K., Flynn, T.C., Gray, K.M., Nabell, L.M., 17β estrodiol acts directly on the clonal osteoblastic cell line UMR-106 (1987) Proc Natl acad sci USA, 84, pp. 6267-6271; Ernst, M., Parker, M.G., Rodan, G.A., Functional estrogen receptor in osteoblastic cells demonstrated by transfection with a receptor gene containing an estrogen response element (1991) Mol endocrinol, 5, pp. 1597-1606; Migliaccio, S., Davis, V.L., Gibson, M.K., Gray, K.T., Korach, K.S., Estrogens modulate the responsiveness of osteoblast like cells (ROS 17/2.8) stably transfected with estrogen receptor (1992) Endocrinology, 130, pp. 2617-2624; Brandi ML. Flavanoids: Biochemical effects and therapeutic applications. Bone Miner, 1992; 19(Suppli 1):S3-14; Lindsay, R., Hart, D.M., Aikken, J.M., McDonald, E.B., Anderson, J.B., Clark, A.C., Long-term prevention of postmenopausal osteoporosis by estrogen: Evidence of an increased bone mass after delayed onset of estrogen treatment (1976) Lancet, 1, pp. 1038-1040; Greenman, D.I., Delongchamp, R.R., Interactive response to DES in CH3 mice (1986) Food Chem Toxicol, 24, pp. 931-934; Yasuo U, Taguchi O,Noumura T,Iguchi T. Effect of sex steroids on the development of sexual dimorphism in mouse innominate bone. 1992, Anat rec.234:541-548; Migliaccio, S., Newbold, R.R., Bullock, B.C., Jefferson, W.J., Sutton Jr, F.G., Mclachlan, J.A., Korach, K.S., Alterations of maternal estrogen levels during gestation affect the skeleton of -female offspring (1996) Endocninology, 137, pp. 2118-2125; Sanyal, A., Ahmad, A., Sastry, M., Calcite growth in Cissus quadrangularis plant extract, a traditional Indian bone healing aid (2005) Current science, 10, pp. 1742-1745

PY - 2007

Y1 - 2007

N2 - Traditional recipes for treatment of physical and mental ailments exist in all major ancient civilizations of the world. One such recipe popular in the Indian subcontinent involves the use of the extract of Cissus quadrangularis plant. Cissus quadrangularis is a shrub, with thick and fleshy quadrangular stem. The roots and stem are most useful for healing of fracture of the bones. The question whether the plant Cissus quadrangularis, which is proved to contain phyto-estrogens will increase the bone mass during fetal growth is not addressed. Accordingly the objective of the present study was to evaluate the effect of alcoholic extract of Cissus quadrangularis on the intrauterine growth of trabeculae in the long bones in rats. Pregnant rats were administered with Ethanol extract of Cissus quadrangularis (CQ), orally, at the dose of 750, mg/kg body weight from 9th day of gestation till delivery. Femur bone of the newborn pups were collected, decalcified and processed for paraffin sectioning. Thickness of cortical bone at mid shaft level, thickness of individual trabeculae were measured using a calibrated ocular micrometer. Results showed a significantly increase in thickness of the cortical bone at mid shaft level compared to control rats. The thickness of individual trabecula was increased significantly. The present study demonstrates enhanced bone formation during fetal growth by CQ, which may be related to rich content of calcium, phosphorus and phytoestrogenic property of the plant.

AB - Traditional recipes for treatment of physical and mental ailments exist in all major ancient civilizations of the world. One such recipe popular in the Indian subcontinent involves the use of the extract of Cissus quadrangularis plant. Cissus quadrangularis is a shrub, with thick and fleshy quadrangular stem. The roots and stem are most useful for healing of fracture of the bones. The question whether the plant Cissus quadrangularis, which is proved to contain phyto-estrogens will increase the bone mass during fetal growth is not addressed. Accordingly the objective of the present study was to evaluate the effect of alcoholic extract of Cissus quadrangularis on the intrauterine growth of trabeculae in the long bones in rats. Pregnant rats were administered with Ethanol extract of Cissus quadrangularis (CQ), orally, at the dose of 750, mg/kg body weight from 9th day of gestation till delivery. Femur bone of the newborn pups were collected, decalcified and processed for paraffin sectioning. Thickness of cortical bone at mid shaft level, thickness of individual trabeculae were measured using a calibrated ocular micrometer. Results showed a significantly increase in thickness of the cortical bone at mid shaft level compared to control rats. The thickness of individual trabecula was increased significantly. The present study demonstrates enhanced bone formation during fetal growth by CQ, which may be related to rich content of calcium, phosphorus and phytoestrogenic property of the plant.

M3 - Article

VL - 3

SP - 190

EP - 202

JO - Pharmacologyonline

JF - Pharmacologyonline

SN - 1827-8620

ER -