Cloning, expression, purification and characterization of chitin deacetylase extremozyme from halophilic Bacillus aryabhattai B8W22

Agriculture & Biology

• chitin deacetylase 81%
• Bacillus (bacteria) 48%
• chitooligosaccharides 44%
• chitin 44%
• molecular cloning 37%
• ethylene glycol 34%
• chitosan 24%
• enzymes 22%
• Bacillus aryabhattai 10%
• extremophiles 8%
• kinetics 8%
• marine ecosystems 8%
• assays 6%
• reproducibility 6%
• affinity chromatography 6%
• esterases 5%
• oligosaccharides 5%
• sediments 5%
• thermal stability 5%
• buffers 5%
• acetates 4%
• polymers 4%
• ribosomal RNA 4%
• screening 4%
• carbohydrates 4%
• enzyme activity 4%
• Escherichia coli 3%
• organisms 3%
• temperature 2%
• genes 2%

Medicine & Life Sciences

• chitin deacetylase 100%
• glycolchitin 55%
• Bacillus 49%
• oligochitosan 46%
• Organism Cloning 45%
• Enzymes 27%
• Chitosan 24%
• Chitin 14%
• Extremophiles 9%
• Kinetics 7%
• Esterases 6%
• Oceans and Seas 6%
• Oligosaccharides 6%
• Ecosystem 6%
• Affinity Chromatography 5%
• Edetic Acid 5%
• 16S Ribosomal RNA 5%
• Acetates 5%
• Buffers 5%
• Polymers 4%
• Carbohydrates 4%
• Temperature 3%
• Escherichia coli 3%
• Databases 3%
• Genes 2%

Earth & Environmental Sciences

• chitin 63%
• cloning 62%
• purification 46%
• enzyme 31%
• ethylene 23%
• substrate 20%
• assay 8%
• extremophile 7%
• kinetics 6%
• industrial production 5%
• EDTA 5%
• enzyme activity 5%
• chromatography 5%
• carbohydrate 4%
• marine ecosystem 4%
• acetate 4%
• screening 4%
• polymer 3%
• gene 3%
• family 3%
• organism 3%
• sea 2%
• sediment 2%
• temperature 1%
• parameter 1%