Cloning, functional expression and characterization of an alkaline protease from Bacillus licheniformis

Ritu Sareen, Uwe T. Bornscheuer, Prashant Mishra

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A gene (apr 46) encoding a protease was cloned from Bacillus licheniformis RSP-09-37. It had an ORF of 1725 bp, encoding a pre-protein of 575 amino acids (63.2 kDa), which was functionally expressed and processed in E. coli JM 109. The mature protein, Apr 46, consists of 500 amino acids with a calculated molecular mass of 55 kDa. This protease shows 29-50% homology to known serine proteases and conserved domains. N-terminal sequencing suggests that Apr 46 protease is identical to a B. licheniformis RSP-09-37 protease, which is further supported by a similar stability in acetonitrile.

Original languageEnglish
Pages (from-to)1901-1907
Number of pages7
JournalBiotechnology Letters
Issue number23-24
Publication statusPublished - 01-12-2005


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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