Collagen characterization in different patterns of bone invasion by OSCC

A histochemical study with picrosirius red and polarizing microscopy

Jyoti Ranjan, Karen Boaz, N. Srikant, K. P. Nandita, Amitha Lewis, Nidhi Manaktala

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: Collagen, the most abundant protein in the body, is not only responsible for maintaining the functional integrity of tissues but is also thought to play an important role in pathogenesis and invasion in oral squamous cell carcinoma (OSCC). Aim: The study aimed to assess the thickness as well as maturation of collagen fibres in erosive and infiltrative patterns of OSCC in order to determine the possible role of stromal components (collagen) in bone invasion. Materials and methods: Histometric analysis was performed on 1350 collagen fibres in 14 cases of OSCC exhibiting either an erosive or an infiltrative pattern of mandibular invasion using polarizing microscope under oil immersion. Two consecutive sections of 5. μm thickness from each sample were taken and stained with haematoxylin and eosin (H&E) and picrosirius red stain, respectively, to enable visualization and counting of thickness of fibres. Results: A significantly higher percentage of thick fibres and a greater amount of green/no colour birefringence were observed in cases of OSCC that exhibited an infiltrative pattern of bone invasion. Conclusion: The stromal component plays an important role in the biological behaviour of a tumour. It is probable that in OSCC the infiltrative pattern of bone invasion induces increased production of loosely packed procollagens, intermediates, or pathological collagen which further aids in the invasion of the tumour into the surrounding bone, worsening the prognosis.

Original languageEnglish
Pages (from-to)258-262
Number of pages5
JournalJournal of Oral and Maxillofacial Surgery, Medicine, and Pathology
Volume27
Issue number2
DOIs
Publication statusPublished - 01-01-2015

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Microscopy
Squamous Cell Carcinoma
Collagen
Bone and Bones
Birefringence
Procollagen
Immersion
Hematoxylin
Eosine Yellowish-(YS)
Neoplasms
Oils
Coloring Agents
Color
C.I. direct red 80
Proteins

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology

Cite this

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title = "Collagen characterization in different patterns of bone invasion by OSCC: A histochemical study with picrosirius red and polarizing microscopy",
abstract = "Introduction: Collagen, the most abundant protein in the body, is not only responsible for maintaining the functional integrity of tissues but is also thought to play an important role in pathogenesis and invasion in oral squamous cell carcinoma (OSCC). Aim: The study aimed to assess the thickness as well as maturation of collagen fibres in erosive and infiltrative patterns of OSCC in order to determine the possible role of stromal components (collagen) in bone invasion. Materials and methods: Histometric analysis was performed on 1350 collagen fibres in 14 cases of OSCC exhibiting either an erosive or an infiltrative pattern of mandibular invasion using polarizing microscope under oil immersion. Two consecutive sections of 5. μm thickness from each sample were taken and stained with haematoxylin and eosin (H&E) and picrosirius red stain, respectively, to enable visualization and counting of thickness of fibres. Results: A significantly higher percentage of thick fibres and a greater amount of green/no colour birefringence were observed in cases of OSCC that exhibited an infiltrative pattern of bone invasion. Conclusion: The stromal component plays an important role in the biological behaviour of a tumour. It is probable that in OSCC the infiltrative pattern of bone invasion induces increased production of loosely packed procollagens, intermediates, or pathological collagen which further aids in the invasion of the tumour into the surrounding bone, worsening the prognosis.",
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Collagen characterization in different patterns of bone invasion by OSCC : A histochemical study with picrosirius red and polarizing microscopy. / Ranjan, Jyoti; Boaz, Karen; Srikant, N.; Nandita, K. P.; Lewis, Amitha; Manaktala, Nidhi.

In: Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology, Vol. 27, No. 2, 01.01.2015, p. 258-262.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Collagen characterization in different patterns of bone invasion by OSCC

T2 - A histochemical study with picrosirius red and polarizing microscopy

AU - Ranjan, Jyoti

AU - Boaz, Karen

AU - Srikant, N.

AU - Nandita, K. P.

AU - Lewis, Amitha

AU - Manaktala, Nidhi

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N2 - Introduction: Collagen, the most abundant protein in the body, is not only responsible for maintaining the functional integrity of tissues but is also thought to play an important role in pathogenesis and invasion in oral squamous cell carcinoma (OSCC). Aim: The study aimed to assess the thickness as well as maturation of collagen fibres in erosive and infiltrative patterns of OSCC in order to determine the possible role of stromal components (collagen) in bone invasion. Materials and methods: Histometric analysis was performed on 1350 collagen fibres in 14 cases of OSCC exhibiting either an erosive or an infiltrative pattern of mandibular invasion using polarizing microscope under oil immersion. Two consecutive sections of 5. μm thickness from each sample were taken and stained with haematoxylin and eosin (H&E) and picrosirius red stain, respectively, to enable visualization and counting of thickness of fibres. Results: A significantly higher percentage of thick fibres and a greater amount of green/no colour birefringence were observed in cases of OSCC that exhibited an infiltrative pattern of bone invasion. Conclusion: The stromal component plays an important role in the biological behaviour of a tumour. It is probable that in OSCC the infiltrative pattern of bone invasion induces increased production of loosely packed procollagens, intermediates, or pathological collagen which further aids in the invasion of the tumour into the surrounding bone, worsening the prognosis.

AB - Introduction: Collagen, the most abundant protein in the body, is not only responsible for maintaining the functional integrity of tissues but is also thought to play an important role in pathogenesis and invasion in oral squamous cell carcinoma (OSCC). Aim: The study aimed to assess the thickness as well as maturation of collagen fibres in erosive and infiltrative patterns of OSCC in order to determine the possible role of stromal components (collagen) in bone invasion. Materials and methods: Histometric analysis was performed on 1350 collagen fibres in 14 cases of OSCC exhibiting either an erosive or an infiltrative pattern of mandibular invasion using polarizing microscope under oil immersion. Two consecutive sections of 5. μm thickness from each sample were taken and stained with haematoxylin and eosin (H&E) and picrosirius red stain, respectively, to enable visualization and counting of thickness of fibres. Results: A significantly higher percentage of thick fibres and a greater amount of green/no colour birefringence were observed in cases of OSCC that exhibited an infiltrative pattern of bone invasion. Conclusion: The stromal component plays an important role in the biological behaviour of a tumour. It is probable that in OSCC the infiltrative pattern of bone invasion induces increased production of loosely packed procollagens, intermediates, or pathological collagen which further aids in the invasion of the tumour into the surrounding bone, worsening the prognosis.

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