yüzdürme ve dansite gradient ayi{dotless}rma metotlari{dotless}ni{dotless}n kombinasyonu DNA hasarli{dotless} spermleri etkili bir şekilde uzaklaşti{dotless}ri{dotless}r

Translated title of the contribution: Combination of swim-up and density gradient separation methods effectively eliminate DNA damaged sperm

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: The aim of this experimental prospective study was to investigate the efficacy of single and combination sperm wash methods for their ability to isolate DNA intact spermatozoa. Material and Methods: Sperm DNA damage was introduced by local testicular irradiation in male mice and the extent of damage was quantified by comet assay. The spermatozoa were subjected to single (swim up or density gradient method) and also a combination of sperm wash techniques. The DNA integrity in various sub-fractions of wash techniques was evaluated. Results: The amount of DNA damaged sperm did not differ between individual fractions when single wash technique was applied. However, a combination of density gradient and swim-up techniques significantly reduced (p<0.01) the number of DNA damaged sperm in the final population. Conclusion: The combination of density gradient separation and swim-up method is effective in eliminating DNA damaged spermatozoa.

Original languageTurkish
Pages (from-to)148-152
Number of pages5
JournalJournal of the Turkish German Gynecology Association
Volume12
Issue number3
DOIs
Publication statusPublished - 2011

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Spermatozoa
DNA
Comet Assay
DNA Damage
Prospective Studies
Population

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

Cite this

@article{a20f6236cbca433d9cb8ab0d3b16628d,
title = "y{\"u}zd{\"u}rme ve dansite gradient ayi{dotless}rma metotlari{dotless}ni{dotless}n kombinasyonu DNA hasarli{dotless} spermleri etkili bir şekilde uzaklaşti{dotless}ri{dotless}r",
abstract = "Objective: The aim of this experimental prospective study was to investigate the efficacy of single and combination sperm wash methods for their ability to isolate DNA intact spermatozoa. Material and Methods: Sperm DNA damage was introduced by local testicular irradiation in male mice and the extent of damage was quantified by comet assay. The spermatozoa were subjected to single (swim up or density gradient method) and also a combination of sperm wash techniques. The DNA integrity in various sub-fractions of wash techniques was evaluated. Results: The amount of DNA damaged sperm did not differ between individual fractions when single wash technique was applied. However, a combination of density gradient and swim-up techniques significantly reduced (p<0.01) the number of DNA damaged sperm in the final population. Conclusion: The combination of density gradient separation and swim-up method is effective in eliminating DNA damaged spermatozoa.",
author = "Surveen Ghumman and Adiga, {Satish Kumar} and Dinesh Upadhya and Guruprasad Kalthur and Varshini Jayaraman and Rao, {Satish Bola} and Pratap Kumar",
year = "2011",
doi = "10.5152/jtgga.2011.35",
language = "Turkish",
volume = "12",
pages = "148--152",
journal = "Journal of the Turkish German Gynecology Association",
issn = "1309-0399",
publisher = "Aves",
number = "3",

}

TY - JOUR

T1 - yüzdürme ve dansite gradient ayi{dotless}rma metotlari{dotless}ni{dotless}n kombinasyonu DNA hasarli{dotless} spermleri etkili bir şekilde uzaklaşti{dotless}ri{dotless}r

AU - Ghumman, Surveen

AU - Adiga, Satish Kumar

AU - Upadhya, Dinesh

AU - Kalthur, Guruprasad

AU - Jayaraman, Varshini

AU - Rao, Satish Bola

AU - Kumar, Pratap

PY - 2011

Y1 - 2011

N2 - Objective: The aim of this experimental prospective study was to investigate the efficacy of single and combination sperm wash methods for their ability to isolate DNA intact spermatozoa. Material and Methods: Sperm DNA damage was introduced by local testicular irradiation in male mice and the extent of damage was quantified by comet assay. The spermatozoa were subjected to single (swim up or density gradient method) and also a combination of sperm wash techniques. The DNA integrity in various sub-fractions of wash techniques was evaluated. Results: The amount of DNA damaged sperm did not differ between individual fractions when single wash technique was applied. However, a combination of density gradient and swim-up techniques significantly reduced (p<0.01) the number of DNA damaged sperm in the final population. Conclusion: The combination of density gradient separation and swim-up method is effective in eliminating DNA damaged spermatozoa.

AB - Objective: The aim of this experimental prospective study was to investigate the efficacy of single and combination sperm wash methods for their ability to isolate DNA intact spermatozoa. Material and Methods: Sperm DNA damage was introduced by local testicular irradiation in male mice and the extent of damage was quantified by comet assay. The spermatozoa were subjected to single (swim up or density gradient method) and also a combination of sperm wash techniques. The DNA integrity in various sub-fractions of wash techniques was evaluated. Results: The amount of DNA damaged sperm did not differ between individual fractions when single wash technique was applied. However, a combination of density gradient and swim-up techniques significantly reduced (p<0.01) the number of DNA damaged sperm in the final population. Conclusion: The combination of density gradient separation and swim-up method is effective in eliminating DNA damaged spermatozoa.

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DO - 10.5152/jtgga.2011.35

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JO - Journal of the Turkish German Gynecology Association

JF - Journal of the Turkish German Gynecology Association

SN - 1309-0399

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ER -