Comparative evaluation of carbon dioxide and carbon dioxide free system in sperm extraction by swim-up technique

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Abstract

Objective: To compare the effectiveness of carbon dioxide and carbon dioxide-free incubation system in extracting motile, morphologically normal and chromatin intact sperm populations from the individual semen samples. Materials and Methods: Semen samples were obtained from normozoospermic men presenting for infertility evaluation. Individual samples were divided into two equal aliquots for sperm wash using CO2 and CO2 free system. Sperm motility, viability, morphology and DNA integrity were assessed in the washed spermatozoa. Sperm DNA integrity was evaluated by acridine orange binding and expressed as the percentage of spermatozoa demonstrating denatured DNA. Results: There was no significant difference in the mean sperm count, motility and morphology of sperms extracted by two systems and both CO2 and CO2 free systems significantly eliminated the number of sperm with denatured DNA. Discussion: The CO2 free system is comparable to conventional CO2 incubation system for swim-up technique in recovering spermatozoa with enhanced motility, morphology and higher DNA integrity.

Original languageEnglish
Pages (from-to)194-197
Number of pages4
JournalJournal of the Turkish German Gynecology Association
Volume8
Issue number2
Publication statusPublished - 06-2007

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Carbon Dioxide
Spermatozoa
DNA
Sperm Count
Sperm Motility
Semen
Acridine Orange
Infertility
Chromatin
Population

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

Cite this

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title = "Comparative evaluation of carbon dioxide and carbon dioxide free system in sperm extraction by swim-up technique",
abstract = "Objective: To compare the effectiveness of carbon dioxide and carbon dioxide-free incubation system in extracting motile, morphologically normal and chromatin intact sperm populations from the individual semen samples. Materials and Methods: Semen samples were obtained from normozoospermic men presenting for infertility evaluation. Individual samples were divided into two equal aliquots for sperm wash using CO2 and CO2 free system. Sperm motility, viability, morphology and DNA integrity were assessed in the washed spermatozoa. Sperm DNA integrity was evaluated by acridine orange binding and expressed as the percentage of spermatozoa demonstrating denatured DNA. Results: There was no significant difference in the mean sperm count, motility and morphology of sperms extracted by two systems and both CO2 and CO2 free systems significantly eliminated the number of sperm with denatured DNA. Discussion: The CO2 free system is comparable to conventional CO2 incubation system for swim-up technique in recovering spermatozoa with enhanced motility, morphology and higher DNA integrity.",
author = "Adiga, {Satish Kumar} and Guruprasad Kalthur and Pratap Kumar",
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T1 - Comparative evaluation of carbon dioxide and carbon dioxide free system in sperm extraction by swim-up technique

AU - Adiga, Satish Kumar

AU - Kalthur, Guruprasad

AU - Kumar, Pratap

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N2 - Objective: To compare the effectiveness of carbon dioxide and carbon dioxide-free incubation system in extracting motile, morphologically normal and chromatin intact sperm populations from the individual semen samples. Materials and Methods: Semen samples were obtained from normozoospermic men presenting for infertility evaluation. Individual samples were divided into two equal aliquots for sperm wash using CO2 and CO2 free system. Sperm motility, viability, morphology and DNA integrity were assessed in the washed spermatozoa. Sperm DNA integrity was evaluated by acridine orange binding and expressed as the percentage of spermatozoa demonstrating denatured DNA. Results: There was no significant difference in the mean sperm count, motility and morphology of sperms extracted by two systems and both CO2 and CO2 free systems significantly eliminated the number of sperm with denatured DNA. Discussion: The CO2 free system is comparable to conventional CO2 incubation system for swim-up technique in recovering spermatozoa with enhanced motility, morphology and higher DNA integrity.

AB - Objective: To compare the effectiveness of carbon dioxide and carbon dioxide-free incubation system in extracting motile, morphologically normal and chromatin intact sperm populations from the individual semen samples. Materials and Methods: Semen samples were obtained from normozoospermic men presenting for infertility evaluation. Individual samples were divided into two equal aliquots for sperm wash using CO2 and CO2 free system. Sperm motility, viability, morphology and DNA integrity were assessed in the washed spermatozoa. Sperm DNA integrity was evaluated by acridine orange binding and expressed as the percentage of spermatozoa demonstrating denatured DNA. Results: There was no significant difference in the mean sperm count, motility and morphology of sperms extracted by two systems and both CO2 and CO2 free systems significantly eliminated the number of sperm with denatured DNA. Discussion: The CO2 free system is comparable to conventional CO2 incubation system for swim-up technique in recovering spermatozoa with enhanced motility, morphology and higher DNA integrity.

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