Comparative evaluation of serological and molecular methods for the diagnosis of scrub typhus in Indian settings

Nitin Gupta, Rama Chaudhry, Sushil K. Kabra, Rakesh Lodha, Bijay Ranjan Mirdha, Bimal Kumar Das, Aparajit Ballay Dey, Vishnubhatla Sreenivas

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The major brunt of scrub typhus is borne by developing countries like India but the disease remains neglected. The rapid progression of this infection to serious complications and associated mortality calls for improved diagnostics. The immunoglobulin (Ig)M immunofluorescence assay (IFA), with all of its limitations remains the gold standard. Of 218 suspected cases from patients enrolled in a 2-year study, 30 cases of scrub typhus were detected by IgM IFA, using a 1:64 dilution. The sensitivities of the IgM enzyme-linked immunosorbent assay, IgM rapid flow assay (RFA), and IgG RFA were found to be 97z, 87z, and 77z, respectively. Their respective specificities were 100z, 100z, and 92z, respectively. The sensitivity and specificity of a nested PCR assay targeting a gene encoding a 56-kDa protein were found to be 50z and 100z, respectively.

Original languageEnglish
Pages (from-to)221-222
Number of pages2
JournalJapanese Journal of Infectious Diseases
Volume70
Issue number2
DOIs
Publication statusPublished - 01-01-2017

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

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