Comparative evaluation of the remineralization potential of Theobromine and Fluoride containing dentifrices using Scanning Electron Microscopy with Energy Dispersive X-Ray Analysis: An in-vitro Study

Nithya Annie Thomas, Priya Shetty, Charisma Thimmaiah, Sowmya B. Shetty, Nimmy Sabu, Kavita Bekal Kripalani

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

The goal of modern dentistry is to prevent caries progression by non-invasive management of non-cavitated carious lesions through remineralization. Theobromine, an alkaloid extract from chocolate has shown a significant remineralizing potential in early carious lesions. To comparatively evaluate the remineralizing efficacy of Theobromine containing dentifrice (TheodentTM)Fluoride containing dentifrice (ClinproTM). In an in-vitro randomized controlled study, 30 sound enamel specimens (4x4x1mm) with a 2x2mm window, prepared from freshly extracted premolars were demineralized using acetate buffer solution (pH 4.2). Specimens were then randomly divided into three groups: Group I-Artificial saliva (control), Group II-TheodentTM, Group III-ClinproTM having 10 specimens each and subjected to remineralisation for a period of 20 and 40 days. Structural and Elemental analysis (Ca/P) was done using Scanning electron microscopy-Energy-dispersive X-ray analysis (SEM-EDAX) at baseline, after demineralization and remineralization. Compared to artificial saliva, TheodentTM and ClinproTM showed significant remineralization as after 20 and 40 days. However, difference between the test groups was not significant. Remineralization in each group was dose-dependent, with more mineralization evident at 40 days. However, none of the groups achieved baseline Ca/P ratios. While, the SEM analysis at 40 days showed homogenous and distinct amorphous deposits on the enamel surface in the test groups, no deposits were seen on the enamel surfaces in the control group. Remineralization potential of TheodentTM is similar to that of ClinproTM. Hence, Theobromine can be considered as a safer future alternative to Fluoride.

Original languageEnglish
Pages (from-to)1314-1320
Number of pages7
JournalJournal of International Dental and Medical Research
Volume14
Issue number4
Publication statusPublished - 2021

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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