Comparative evaluation of two cold staining methods with the ziehl-neelsen method for the diagnosis of tuberculosis

Soham Gupta, Vishnu Prasad, Indira Bairy, S. Muralidharan

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Abstract

In developing countries pulmonary tuberculosis is usually diagnosed by detecting acid-fast bacilli (AFB) in sputum using a Ziehl-Neelsen (Z-N) staining method. However, in the field the traditional method of staining is difficult to carry out. This study evaluates the efficiency of two cold staining methods, namely Gabbeťs and a modified two reagent cold staining method compared with the Z-N taken as the gold standard. Triplicate smears were prepared from 267 sputum samples and stained by the Z-N, Gabbeťs, and a modified cold staining method, the smears were positive for AFB in 21 (7.87%), 18 (6.74%) and 19 (7.12%), respectively. The sensitivities for the Gabbeťs and modified cold stain were 85.7% and 90.5%, respectively. The positive agreement between the Z-N and Gabbeťs (92.3%) and Z-N and modified cold stain (95%) were good. The modified cold staining method was less time consuming and easier to perform in the field.

Original languageEnglish
Pages (from-to)765-769
Number of pages5
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume40
Issue number4
Publication statusPublished - 01-07-2009

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Tuberculosis
Staining and Labeling
Sputum
Bacillus
Coloring Agents
Acids
Pulmonary Tuberculosis
Developing Countries

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

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title = "Comparative evaluation of two cold staining methods with the ziehl-neelsen method for the diagnosis of tuberculosis",
abstract = "In developing countries pulmonary tuberculosis is usually diagnosed by detecting acid-fast bacilli (AFB) in sputum using a Ziehl-Neelsen (Z-N) staining method. However, in the field the traditional method of staining is difficult to carry out. This study evaluates the efficiency of two cold staining methods, namely Gabbeťs and a modified two reagent cold staining method compared with the Z-N taken as the gold standard. Triplicate smears were prepared from 267 sputum samples and stained by the Z-N, Gabbeťs, and a modified cold staining method, the smears were positive for AFB in 21 (7.87{\%}), 18 (6.74{\%}) and 19 (7.12{\%}), respectively. The sensitivities for the Gabbeťs and modified cold stain were 85.7{\%} and 90.5{\%}, respectively. The positive agreement between the Z-N and Gabbeťs (92.3{\%}) and Z-N and modified cold stain (95{\%}) were good. The modified cold staining method was less time consuming and easier to perform in the field.",
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AU - Prasad, Vishnu

AU - Bairy, Indira

AU - Muralidharan, S.

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