Comparison of darkground microscopy with serological tests in the diagnosis of leptospirosis with hepatorenal involvement-A preliminary study

P. Sugandhi Rao, Shashibhushan, P. G. Shivananda

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

Darkground Microscopy (DGM) was initially done on Plasma in the Department of Microbiology, Kasturba Medical College, Manipal for 44 cases of suspected Leptospiral hepatorenal involvement. A total of 60 sera were collected from these patients and stored at -70°C Passiive haemagglutination test (PHA) was performed on 25 sera and latex agglutination test on 35 sera (Leptospira antigen Seiken) Leptospira was detected by Darkground Microscopy in 12 patients (27.27%). Serology was positive in 16 samples (36.36%) of which 7 (15.90%) by PHA and 9 by Latex agglutination (20.45%). Serological tests confirmed the findings of Darkground Microscopy. DCM was found to be simple and rapid and could be performed on all suspected patients,.

Original languageEnglish
Pages (from-to)427-429
Number of pages3
JournalIndian Journal of Pathology and Microbiology
Volume41
Issue number4
Publication statusPublished - 01-10-1998
Externally publishedYes

Fingerprint

Leptospirosis
Serologic Tests
Hemagglutination Tests
Leptospira
Microscopy
Serum
Latex Fixation Tests
Agglutination
Latex
Serology
Microbiology
Antigens

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Microbiology (medical)

Cite this

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title = "Comparison of darkground microscopy with serological tests in the diagnosis of leptospirosis with hepatorenal involvement-A preliminary study",
abstract = "Darkground Microscopy (DGM) was initially done on Plasma in the Department of Microbiology, Kasturba Medical College, Manipal for 44 cases of suspected Leptospiral hepatorenal involvement. A total of 60 sera were collected from these patients and stored at -70°C Passiive haemagglutination test (PHA) was performed on 25 sera and latex agglutination test on 35 sera (Leptospira antigen Seiken) Leptospira was detected by Darkground Microscopy in 12 patients (27.27{\%}). Serology was positive in 16 samples (36.36{\%}) of which 7 (15.90{\%}) by PHA and 9 by Latex agglutination (20.45{\%}). Serological tests confirmed the findings of Darkground Microscopy. DCM was found to be simple and rapid and could be performed on all suspected patients,.",
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Comparison of darkground microscopy with serological tests in the diagnosis of leptospirosis with hepatorenal involvement-A preliminary study. / Sugandhi Rao, P.; Shashibhushan; Shivananda, P. G.

In: Indian Journal of Pathology and Microbiology, Vol. 41, No. 4, 01.10.1998, p. 427-429.

Research output: Contribution to journalReview article

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T1 - Comparison of darkground microscopy with serological tests in the diagnosis of leptospirosis with hepatorenal involvement-A preliminary study

AU - Sugandhi Rao, P.

AU - Shashibhushan,

AU - Shivananda, P. G.

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