Comparison of darkground microscopy with serological tests in the diagnosis of leptospirosis with hepatorenal involvement-A preliminary study

P. Sugandhi Rao, Shashibhushan, P. G. Shivananda

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Abstract

Darkground Microscopy (DGM) was initially done on Plasma in the Department of Microbiology, Kasturba Medical College, Manipal for 44 cases of suspected Leptospiral hepatorenal involvement. A total of 60 sera were collected from these patients and stored at -70°C Passiive haemagglutination test (PHA) was performed on 25 sera and latex agglutination test on 35 sera (Leptospira antigen Seiken) Leptospira was detected by Darkground Microscopy in 12 patients (27.27%). Serology was positive in 16 samples (36.36%) of which 7 (15.90%) by PHA and 9 by Latex agglutination (20.45%). Serological tests confirmed the findings of Darkground Microscopy. DCM was found to be simple and rapid and could be performed on all suspected patients,.

Original languageEnglish
Pages (from-to)427-429
Number of pages3
JournalIndian Journal of Pathology and Microbiology
Volume41
Issue number4
Publication statusPublished - 01-10-1998
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Microbiology (medical)

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