Comparison of immuno-phenotypes of stem cells from human dental pulp and periodontal ligament

Deepa Ponnaiyan, K. M. Bhat, G. S. Bhat

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

It has been established that human dental pulp and periodontal ligament contain a population of mesenchymal stem cells (MSCs). However, the phenotypic analysis in terms of putative stem cell markers expressed by these stem cell populations is incomplete. It is relevant to understand whether stem cells derived from closely related tissues are programmed differently. The aim of the present study is to analyze whether these stem cells depict distinct characteristics by gaining insight into differences in their immunophenotype. Dental pulp and periodontal ligament tissue samples were obtained from extracted impacted wisdom teeth. Cell cultures were analyzed for surface and intracellular markers by indirect immunoflourescence. Detailed immunophenotype analysis was carried out by flow cytometry using relevant markers. The present study data shows dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) expressed embryonic stem (ES) cell markers Oct-4, Nanog and mesodermal marker Vimentin by indirect immunoflourescence. PDLSCs, however, had a weak expression of Nanog. Immunophenotyping revealed strong expression of MSC markers (CD73, CD90) in DPSCs and PDLSCs. Differences were observed in expression of sternness-related markers. DPSCs displayed increased percentages of SSEA4, CD13 and CD166 and decreased CD9 expression compared to PDLSCs. Both stem cells express common MSC markers, different levels of expression suggests there might be more than one stem cell population existing within these tissues which differ in their embryonic status, and DPSCs are a more primitive stem cell population in comparison to PDLSCs.

Original languageEnglish
Pages (from-to)127-134
Number of pages8
JournalInternational Journal of Immunopathology and Pharmacology
Volume25
Issue number1
DOIs
Publication statusPublished - 01-01-2012

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Dental Pulp
Periodontal Ligament
Stem Cells
Phenotype
Mesenchymal Stromal Cells
Population
Impacted Tooth
Immunophenotyping
Third Molar
Vimentin
Embryonic Stem Cells

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Pharmacology

Cite this

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Comparison of immuno-phenotypes of stem cells from human dental pulp and periodontal ligament. / Ponnaiyan, Deepa; Bhat, K. M.; Bhat, G. S.

In: International Journal of Immunopathology and Pharmacology, Vol. 25, No. 1, 01.01.2012, p. 127-134.

Research output: Contribution to journalArticle

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