Comprehensive DNA copy number profile and BAC library construction of an Indian individual

Sanjiban Chakrabarty, Reena R. D'Souza, Ravishankara Bellampalli, Harish Rotti, Abdul V. Saadi, Puthiya M. Gopinath, Raviraja V. Acharya, Periyasamy Govindaraj, Kumarasamy Thangaraj, Kapaettu Satyamoorthy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bacterial artificial chromosomes (BACs) are used in genomic variation studies due to their capacity to carry a large insert, their high clonal stability, low rate of chimerism and ease of manipulation. In the present study, an attempt was made to create the first genomic BAC library of an anonymous Indian male (IMBL4) consisting of 100,224 clones covering the human genome more than three times. Restriction mapping of 255 BAC clones by pulse field gel electrophoresis confirmed an average insert size of 120. kb. The library was screened by PCR using . SHANK3 (SH3 and multiple ankyrin repeat domains 3) and . OLFM3 (olfactomedin 3) specific primers. A selection of clones was analyzed by fluorescent . in situ hybridization (FISH) and sequencing. Fine mapping of copy number variable regions by array based comparative genomic hybridization identified 467 CNVRs in the IMBL4 genome. The IMBL4 BAC library represents the first cataloged Indian genome resource for applications in basic and clinical research.

Original languageEnglish
Pages (from-to)186-193
Number of pages8
JournalGene
Volume500
Issue number2
DOIs
Publication statusPublished - 01-06-2012

Fingerprint

Bacterial Artificial Chromosomes
Clone Cells
DNA
Genome
Ankyrin Repeat
Restriction Mapping
Chimerism
Comparative Genomic Hybridization
Human Genome
Libraries
In Situ Hybridization
Electrophoresis
Gels
Polymerase Chain Reaction
Research

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Chakrabarty, Sanjiban ; D'Souza, Reena R. ; Bellampalli, Ravishankara ; Rotti, Harish ; Saadi, Abdul V. ; Gopinath, Puthiya M. ; Acharya, Raviraja V. ; Govindaraj, Periyasamy ; Thangaraj, Kumarasamy ; Satyamoorthy, Kapaettu. / Comprehensive DNA copy number profile and BAC library construction of an Indian individual. In: Gene. 2012 ; Vol. 500, No. 2. pp. 186-193.
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Comprehensive DNA copy number profile and BAC library construction of an Indian individual. / Chakrabarty, Sanjiban; D'Souza, Reena R.; Bellampalli, Ravishankara; Rotti, Harish; Saadi, Abdul V.; Gopinath, Puthiya M.; Acharya, Raviraja V.; Govindaraj, Periyasamy; Thangaraj, Kumarasamy; Satyamoorthy, Kapaettu.

In: Gene, Vol. 500, No. 2, 01.06.2012, p. 186-193.

Research output: Contribution to journalArticle

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