Abstract

Eryptosis is the suicidal destruction-process of erythrocytes, much like apoptosis of nucleated cells, in the course of which the stressed red cell undergoes cell-shrinkage, vesiculation and externalization of membrane phosphatidylserine. Currently, there exist numerous methods to detect eryptosis, both morphometrically and biochemically. This study aimed to design a simple but sensitive, automated computerized approach to instantaneously detect eryptotic red cells and quantify their hallmark morphological characteristics. Red cells from 17 healthy volunteers were exposed to normal Ringer and hyperosmotic stress with sodium chloride, following which morphometric comparisons were conducted from their photomicrographs. The proposed method was found to significantly detect and differentiate normal and eryptotic red cells, based on variations in their structural markers. The receiver operating characteristic curve analysis for each of the markers showed a significant discriminatory accuracy with high sensitivity, specificity and area under the curve values. The software-based technique was then validated with RBCs in malaria. This model, quantifies eryptosis morphometrically in real-time, with minimal manual intervention, providing a new window to explore eryptosis triggered by different stressors and diseases and can find wide application in laboratories of hematology, blood banks and medical research.

Original languageEnglish
Article number1230
JournalFrontiers in Physiology
Volume10
Issue numberSEP
DOIs
Publication statusPublished - 01-09-2019

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Blood Banks
Phosphatidylserines
Hematology
Sodium Chloride
ROC Curve
Malaria
Area Under Curve
Eryptosis
Biomedical Research
Healthy Volunteers
Software
Erythrocytes
Apoptosis
Sensitivity and Specificity
Membranes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

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title = "Computerized morphometric analysis of eryptosis",
abstract = "Eryptosis is the suicidal destruction-process of erythrocytes, much like apoptosis of nucleated cells, in the course of which the stressed red cell undergoes cell-shrinkage, vesiculation and externalization of membrane phosphatidylserine. Currently, there exist numerous methods to detect eryptosis, both morphometrically and biochemically. This study aimed to design a simple but sensitive, automated computerized approach to instantaneously detect eryptotic red cells and quantify their hallmark morphological characteristics. Red cells from 17 healthy volunteers were exposed to normal Ringer and hyperosmotic stress with sodium chloride, following which morphometric comparisons were conducted from their photomicrographs. The proposed method was found to significantly detect and differentiate normal and eryptotic red cells, based on variations in their structural markers. The receiver operating characteristic curve analysis for each of the markers showed a significant discriminatory accuracy with high sensitivity, specificity and area under the curve values. The software-based technique was then validated with RBCs in malaria. This model, quantifies eryptosis morphometrically in real-time, with minimal manual intervention, providing a new window to explore eryptosis triggered by different stressors and diseases and can find wide application in laboratories of hematology, blood banks and medical research.",
author = "Jacob, {Sanu Susan} and Keerthana Prasad and Pragna Rao and Asha Kamath and Hegde, {Roopa B.} and Baby, {Prathap M.} and Rao, {Raghavendra K.}",
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Computerized morphometric analysis of eryptosis. / Jacob, Sanu Susan; Prasad, Keerthana; Rao, Pragna; Kamath, Asha; Hegde, Roopa B.; Baby, Prathap M.; Rao, Raghavendra K.

In: Frontiers in Physiology, Vol. 10, No. SEP, 1230, 01.09.2019.

Research output: Contribution to journalArticle

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AU - Jacob, Sanu Susan

AU - Prasad, Keerthana

AU - Rao, Pragna

AU - Kamath, Asha

AU - Hegde, Roopa B.

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AU - Rao, Raghavendra K.

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