Coronary artery disease and the frequencies of MTHFR and PON1 gene polymorphism studies in a varied population of Hyderabad, Telangana region in south India

Kavitha Matam, Imran Ali Khan, Qurratulain Hasan, Pragna Rao

Research output: Contribution to journalArticle

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Abstract

Coronary artery disease (CAD) also known as coronary heart disease is one of the leading causes of death and disability worldwide. Genetic and environmental factors play an important role in the pathogenesis and progression of CAD. The aim of this study was to evaluate the combined contribution of 3 gene polymorphisms to the risk of CAD and gene-gene interaction in the south Indian population. In this case-control study, 200 cases of CAD and 200 healthy controls were recruited. We studied 3 well known genetic polymorphisms of MTHFR (C677T; rs1801133), PON1 (Q192R; rs662) and ACE (I/D: rs4646994) in relation to CAD in South Indian population. Polymerase chain reaction (PCR) was carried out and followed by the restriction fragment length polymorphism and agarose gel electrophoresis. Genotypes of MTHFR C677T, CT and CT+TT, and PON1 Q192R QR were associated with the risk of CAD (C677T CT+TT vs CC: odds ratio [OR]. =. 3.3, 95% confidence intervals [CI]. =. 1.8-6.2; p=. 0.00001), (CT vs CC: OR. =. 3.2, 95%CI. =. 1.8-5.6; p=. 0.00003), and (Q192R QQ vs QR: OR. =. 2.1, 95%CI. =. 1.1-3.9; p=. 0.03). The allele frequencies for T vs C: OR. =. 3.1, 95%CI. =. 1.8-5.3; p=. 0.00001 and R vs Q: OR. =. 1.3, 95% CI. =. 1.0-1.7 p=. 0.03. Multifactor dimensionality reduction (MDR) analysis was carried out with the combination of three genes and the results indicate that MDR analysis showed that, PON1 gene polymorphism formed a significant model in predicting the CAD risk in south Indian population.

Original languageEnglish
Pages (from-to)143-150
Number of pages8
JournalJournal of King Saud University - Science
Volume27
Issue number2
DOIs
Publication statusPublished - 01-04-2015

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Coronary Artery Disease
India
Odds Ratio
Confidence Intervals
Population
Genes
Multifactor Dimensionality Reduction
Agar Gel Electrophoresis
Genetic Polymorphisms
Gene Frequency
Restriction Fragment Length Polymorphisms
Coronary Disease
Case-Control Studies
Cause of Death
Genotype
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{19369d1b3b9546a69d8f787b36f263c9,
title = "Coronary artery disease and the frequencies of MTHFR and PON1 gene polymorphism studies in a varied population of Hyderabad, Telangana region in south India",
abstract = "Coronary artery disease (CAD) also known as coronary heart disease is one of the leading causes of death and disability worldwide. Genetic and environmental factors play an important role in the pathogenesis and progression of CAD. The aim of this study was to evaluate the combined contribution of 3 gene polymorphisms to the risk of CAD and gene-gene interaction in the south Indian population. In this case-control study, 200 cases of CAD and 200 healthy controls were recruited. We studied 3 well known genetic polymorphisms of MTHFR (C677T; rs1801133), PON1 (Q192R; rs662) and ACE (I/D: rs4646994) in relation to CAD in South Indian population. Polymerase chain reaction (PCR) was carried out and followed by the restriction fragment length polymorphism and agarose gel electrophoresis. Genotypes of MTHFR C677T, CT and CT+TT, and PON1 Q192R QR were associated with the risk of CAD (C677T CT+TT vs CC: odds ratio [OR]. =. 3.3, 95{\%} confidence intervals [CI]. =. 1.8-6.2; p=. 0.00001), (CT vs CC: OR. =. 3.2, 95{\%}CI. =. 1.8-5.6; p=. 0.00003), and (Q192R QQ vs QR: OR. =. 2.1, 95{\%}CI. =. 1.1-3.9; p=. 0.03). The allele frequencies for T vs C: OR. =. 3.1, 95{\%}CI. =. 1.8-5.3; p=. 0.00001 and R vs Q: OR. =. 1.3, 95{\%} CI. =. 1.0-1.7 p=. 0.03. Multifactor dimensionality reduction (MDR) analysis was carried out with the combination of three genes and the results indicate that MDR analysis showed that, PON1 gene polymorphism formed a significant model in predicting the CAD risk in south Indian population.",
author = "Kavitha Matam and Khan, {Imran Ali} and Qurratulain Hasan and Pragna Rao",
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Coronary artery disease and the frequencies of MTHFR and PON1 gene polymorphism studies in a varied population of Hyderabad, Telangana region in south India. / Matam, Kavitha; Khan, Imran Ali; Hasan, Qurratulain; Rao, Pragna.

In: Journal of King Saud University - Science, Vol. 27, No. 2, 01.04.2015, p. 143-150.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Coronary artery disease and the frequencies of MTHFR and PON1 gene polymorphism studies in a varied population of Hyderabad, Telangana region in south India

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AU - Rao, Pragna

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N2 - Coronary artery disease (CAD) also known as coronary heart disease is one of the leading causes of death and disability worldwide. Genetic and environmental factors play an important role in the pathogenesis and progression of CAD. The aim of this study was to evaluate the combined contribution of 3 gene polymorphisms to the risk of CAD and gene-gene interaction in the south Indian population. In this case-control study, 200 cases of CAD and 200 healthy controls were recruited. We studied 3 well known genetic polymorphisms of MTHFR (C677T; rs1801133), PON1 (Q192R; rs662) and ACE (I/D: rs4646994) in relation to CAD in South Indian population. Polymerase chain reaction (PCR) was carried out and followed by the restriction fragment length polymorphism and agarose gel electrophoresis. Genotypes of MTHFR C677T, CT and CT+TT, and PON1 Q192R QR were associated with the risk of CAD (C677T CT+TT vs CC: odds ratio [OR]. =. 3.3, 95% confidence intervals [CI]. =. 1.8-6.2; p=. 0.00001), (CT vs CC: OR. =. 3.2, 95%CI. =. 1.8-5.6; p=. 0.00003), and (Q192R QQ vs QR: OR. =. 2.1, 95%CI. =. 1.1-3.9; p=. 0.03). The allele frequencies for T vs C: OR. =. 3.1, 95%CI. =. 1.8-5.3; p=. 0.00001 and R vs Q: OR. =. 1.3, 95% CI. =. 1.0-1.7 p=. 0.03. Multifactor dimensionality reduction (MDR) analysis was carried out with the combination of three genes and the results indicate that MDR analysis showed that, PON1 gene polymorphism formed a significant model in predicting the CAD risk in south Indian population.

AB - Coronary artery disease (CAD) also known as coronary heart disease is one of the leading causes of death and disability worldwide. Genetic and environmental factors play an important role in the pathogenesis and progression of CAD. The aim of this study was to evaluate the combined contribution of 3 gene polymorphisms to the risk of CAD and gene-gene interaction in the south Indian population. In this case-control study, 200 cases of CAD and 200 healthy controls were recruited. We studied 3 well known genetic polymorphisms of MTHFR (C677T; rs1801133), PON1 (Q192R; rs662) and ACE (I/D: rs4646994) in relation to CAD in South Indian population. Polymerase chain reaction (PCR) was carried out and followed by the restriction fragment length polymorphism and agarose gel electrophoresis. Genotypes of MTHFR C677T, CT and CT+TT, and PON1 Q192R QR were associated with the risk of CAD (C677T CT+TT vs CC: odds ratio [OR]. =. 3.3, 95% confidence intervals [CI]. =. 1.8-6.2; p=. 0.00001), (CT vs CC: OR. =. 3.2, 95%CI. =. 1.8-5.6; p=. 0.00003), and (Q192R QQ vs QR: OR. =. 2.1, 95%CI. =. 1.1-3.9; p=. 0.03). The allele frequencies for T vs C: OR. =. 3.1, 95%CI. =. 1.8-5.3; p=. 0.00001 and R vs Q: OR. =. 1.3, 95% CI. =. 1.0-1.7 p=. 0.03. Multifactor dimensionality reduction (MDR) analysis was carried out with the combination of three genes and the results indicate that MDR analysis showed that, PON1 gene polymorphism formed a significant model in predicting the CAD risk in south Indian population.

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