Correlation of micronuclei-induction with the cell survival in HeLa cells treated with a base analogue, azidothymidine (AZT) before exposure to different doses of γ-radiation

Ganesh Chandra Jagetia, R. Aruna

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effect of 0.1 μM azidothymidine (AZT) a pyrimidine analogue has been studied on the growth kinetics, cell survival and micronuclei formation in HeLa cells exposed to 0, 0.25, 0.5, 1, 2 and 3 Gy of 60Co γ-radiation. The AZT pretreatment resulted in a significant decline in the cell growth kinetics, cell survival and cell proliferation indices when compared with the PBS+irradiation group at 20, 30 and 40 h post-irradiation. Conversely, the frequency of micronucleated binucleate cells (MNBNC) elevated in a dose dependent manner in both PBS+irradiation and AZT+irradiation group. This elevation in MNBNC-induction was significantly higher in the latter when compared with the former group at all post-irradiation scoring time periods studied. The dose-response relationship for micronuclei induction for both the PBS+irrradiation and AZT+irradiation groups was linear. The biological response was studied by correlating the cell survival with MNBNC-induction. The cell survival and MNBNC-induction showed a close but inverse relationship and this relationship gave a best fit on the linear quadratic model.

Original languageEnglish
Pages (from-to)33-43
Number of pages11
JournalToxicology Letters
Volume139
Issue number1
DOIs
Publication statusPublished - 20-03-2003
Externally publishedYes

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Zidovudine
HeLa Cells
Dosimetry
Cell Survival
Cells
Irradiation
Radiation
Growth kinetics
Radiation Dosage
Growth
Cell proliferation
Cell growth
Linear Models
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

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title = "Correlation of micronuclei-induction with the cell survival in HeLa cells treated with a base analogue, azidothymidine (AZT) before exposure to different doses of γ-radiation",
abstract = "The effect of 0.1 μM azidothymidine (AZT) a pyrimidine analogue has been studied on the growth kinetics, cell survival and micronuclei formation in HeLa cells exposed to 0, 0.25, 0.5, 1, 2 and 3 Gy of 60Co γ-radiation. The AZT pretreatment resulted in a significant decline in the cell growth kinetics, cell survival and cell proliferation indices when compared with the PBS+irradiation group at 20, 30 and 40 h post-irradiation. Conversely, the frequency of micronucleated binucleate cells (MNBNC) elevated in a dose dependent manner in both PBS+irradiation and AZT+irradiation group. This elevation in MNBNC-induction was significantly higher in the latter when compared with the former group at all post-irradiation scoring time periods studied. The dose-response relationship for micronuclei induction for both the PBS+irrradiation and AZT+irradiation groups was linear. The biological response was studied by correlating the cell survival with MNBNC-induction. The cell survival and MNBNC-induction showed a close but inverse relationship and this relationship gave a best fit on the linear quadratic model.",
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Correlation of micronuclei-induction with the cell survival in HeLa cells treated with a base analogue, azidothymidine (AZT) before exposure to different doses of γ-radiation. / Jagetia, Ganesh Chandra; Aruna, R.

In: Toxicology Letters, Vol. 139, No. 1, 20.03.2003, p. 33-43.

Research output: Contribution to journalArticle

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