Correlation of plasma lipid profile with salivary oxidative stress markers in type II diabetes mellitus patients

S. G. Rajeshwari, Afreen Arshad Choudhry, Arpitha Gururaja, Krishnananda Prabhu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Introduction: Diabetes is known to be associated with greater production of reactive oxygen species (ROS) like malondialdehyde (MDA) and decreased antioxidants like total thiols and its estimation in plasma is used in monitoring the redox status. The aim of this study was to analyse the association between plasma lipid profile parameters like HDL and LDL with salivary MDA and thiols in diabetic patients. Materials and Methods: Sixty subjects between the age group 35- 70 years who were diagnosed with type 2 diabetes mellitus based on fasting blood glucose and glycated haemoglobin levels who attended the dental OPD at Kasturba Medical College and Hospital, Manipal consented to participate in this study. Plasma glucose, HDL and LDL were estimated in Cobas autoanalyser by hexokinase method, homogenous enzymatic colorimetric assay and Friedwald's formula respectively. Assessment of glycated hemoglobin was by ion exchange chromatography, MDA by thiobarbituric acid as a substrate and thiols by Ellmann's manual method in plasma and saliva. Results: The association of plasma LDL with salivary MDA was found to be positive and significant and that with salivary thiols was negative and significant. Also, the association of plasma HDL with salivary MDA was found to be negative and significant and that with salivary thiols was positive and significant. Conclusion: Results indicate the potential of saliva as a tool to monitor prognosis of diabetes.

Original languageEnglish
JournalJournal of Clinical and Diagnostic Research
Volume8
Issue number6
DOIs
Publication statusPublished - 20-06-2014

Fingerprint

Oxidative stress
Medical problems
Type 2 Diabetes Mellitus
Malondialdehyde
Sulfhydryl Compounds
Oxidative Stress
Lipids
Plasmas
Glycosylated Hemoglobin A
Association reactions
Saliva
Hexokinase
Ion Exchange Chromatography
Enzyme Assays
Chromatography
Oxidation-Reduction
Blood Glucose
Assays
Fasting
Reactive Oxygen Species

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

@article{d07fa4fc39324139813941856d248b2d,
title = "Correlation of plasma lipid profile with salivary oxidative stress markers in type II diabetes mellitus patients",
abstract = "Introduction: Diabetes is known to be associated with greater production of reactive oxygen species (ROS) like malondialdehyde (MDA) and decreased antioxidants like total thiols and its estimation in plasma is used in monitoring the redox status. The aim of this study was to analyse the association between plasma lipid profile parameters like HDL and LDL with salivary MDA and thiols in diabetic patients. Materials and Methods: Sixty subjects between the age group 35- 70 years who were diagnosed with type 2 diabetes mellitus based on fasting blood glucose and glycated haemoglobin levels who attended the dental OPD at Kasturba Medical College and Hospital, Manipal consented to participate in this study. Plasma glucose, HDL and LDL were estimated in Cobas autoanalyser by hexokinase method, homogenous enzymatic colorimetric assay and Friedwald's formula respectively. Assessment of glycated hemoglobin was by ion exchange chromatography, MDA by thiobarbituric acid as a substrate and thiols by Ellmann's manual method in plasma and saliva. Results: The association of plasma LDL with salivary MDA was found to be positive and significant and that with salivary thiols was negative and significant. Also, the association of plasma HDL with salivary MDA was found to be negative and significant and that with salivary thiols was positive and significant. Conclusion: Results indicate the potential of saliva as a tool to monitor prognosis of diabetes.",
author = "Rajeshwari, {S. G.} and Choudhry, {Afreen Arshad} and Arpitha Gururaja and Krishnananda Prabhu",
year = "2014",
month = "6",
day = "20",
doi = "10.7860/JCDR/2014/8233.4451",
language = "English",
volume = "8",
journal = "Journal of Clinical and Diagnostic Research",
issn = "2249-782X",
publisher = "Journal of Clinical and Diagnostic Research",
number = "6",

}

Correlation of plasma lipid profile with salivary oxidative stress markers in type II diabetes mellitus patients. / Rajeshwari, S. G.; Choudhry, Afreen Arshad; Gururaja, Arpitha; Prabhu, Krishnananda.

In: Journal of Clinical and Diagnostic Research, Vol. 8, No. 6, 20.06.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Correlation of plasma lipid profile with salivary oxidative stress markers in type II diabetes mellitus patients

AU - Rajeshwari, S. G.

AU - Choudhry, Afreen Arshad

AU - Gururaja, Arpitha

AU - Prabhu, Krishnananda

PY - 2014/6/20

Y1 - 2014/6/20

N2 - Introduction: Diabetes is known to be associated with greater production of reactive oxygen species (ROS) like malondialdehyde (MDA) and decreased antioxidants like total thiols and its estimation in plasma is used in monitoring the redox status. The aim of this study was to analyse the association between plasma lipid profile parameters like HDL and LDL with salivary MDA and thiols in diabetic patients. Materials and Methods: Sixty subjects between the age group 35- 70 years who were diagnosed with type 2 diabetes mellitus based on fasting blood glucose and glycated haemoglobin levels who attended the dental OPD at Kasturba Medical College and Hospital, Manipal consented to participate in this study. Plasma glucose, HDL and LDL were estimated in Cobas autoanalyser by hexokinase method, homogenous enzymatic colorimetric assay and Friedwald's formula respectively. Assessment of glycated hemoglobin was by ion exchange chromatography, MDA by thiobarbituric acid as a substrate and thiols by Ellmann's manual method in plasma and saliva. Results: The association of plasma LDL with salivary MDA was found to be positive and significant and that with salivary thiols was negative and significant. Also, the association of plasma HDL with salivary MDA was found to be negative and significant and that with salivary thiols was positive and significant. Conclusion: Results indicate the potential of saliva as a tool to monitor prognosis of diabetes.

AB - Introduction: Diabetes is known to be associated with greater production of reactive oxygen species (ROS) like malondialdehyde (MDA) and decreased antioxidants like total thiols and its estimation in plasma is used in monitoring the redox status. The aim of this study was to analyse the association between plasma lipid profile parameters like HDL and LDL with salivary MDA and thiols in diabetic patients. Materials and Methods: Sixty subjects between the age group 35- 70 years who were diagnosed with type 2 diabetes mellitus based on fasting blood glucose and glycated haemoglobin levels who attended the dental OPD at Kasturba Medical College and Hospital, Manipal consented to participate in this study. Plasma glucose, HDL and LDL were estimated in Cobas autoanalyser by hexokinase method, homogenous enzymatic colorimetric assay and Friedwald's formula respectively. Assessment of glycated hemoglobin was by ion exchange chromatography, MDA by thiobarbituric acid as a substrate and thiols by Ellmann's manual method in plasma and saliva. Results: The association of plasma LDL with salivary MDA was found to be positive and significant and that with salivary thiols was negative and significant. Also, the association of plasma HDL with salivary MDA was found to be negative and significant and that with salivary thiols was positive and significant. Conclusion: Results indicate the potential of saliva as a tool to monitor prognosis of diabetes.

UR - http://www.scopus.com/inward/record.url?scp=84903218270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903218270&partnerID=8YFLogxK

U2 - 10.7860/JCDR/2014/8233.4451

DO - 10.7860/JCDR/2014/8233.4451

M3 - Article

AN - SCOPUS:84903218270

VL - 8

JO - Journal of Clinical and Diagnostic Research

JF - Journal of Clinical and Diagnostic Research

SN - 2249-782X

IS - 6

ER -