Correlation of Red Blood Cell Acetylcholinesterase Enzyme Activity with Various RBC Indices

Shalvika Gupta, Vijetha Shenoy Belle, Ramya Kumbarakeri Rajashekhar, Sushma Jogi, RV Krishnananda Prabhu

Research output: Contribution to journalArticle

Abstract

Cholinesterases belongs to class hydrolases. There are two types acetylcholinesterase and butyryl cholinesterase. Acetylcholinesterase present in nerve endings and also in the RBC membrane. It helps to maintain the shape and size of RBCs. Any change in shape and size of RBCs may affect the activity of Acetylcholinesterase. Thus this study aimed to estimate RBCs Acetylcholinesterase enzyme activity in various types of anemias and correlate the RBCs Acetylcholinesterase enzyme activity with various hematological indices such as Erythrocyte Sedimentation Rate (ESR), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV), Red cell Distribution Width (RDW) etc. After obtaining ethical approval from Institutional ethics committee total of 100 samples were collected from Clinical Biochemistry laboratory, Kasturba Medical College, Manipal, Manipal University. 25 were having normal RBC indices, 12 with hemolytic anemia, 26 with microcytic anemia and 26 with macrocytic anemia based on peripheral smear report and RBC indices. Acetylcholinesterase were measured using Ellman’s method. RBC acetylcholinesterase activity was significantly increased in microcytic anemia (58.13 ± 5.4) and macrocytic anemia (76.87 ± 6.7) than normal group (37.62 ± 2.71). Also increased RBC acetylcholinesterase was seen in hemolytic anemia (48.11 ± 5.18) but the increase is not statistically significant. RBC acetylcholinesterase correlated negatively with hemoglobin (r = −0.356, p = 0.001) and positively with RDW (r = 0.31, p = 0.003). To conclude RBC acetylcholinesterase activity can be used as one of the potential marker for various types of anemia.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalIndian Journal of Clinical Biochemistry
DOIs
Publication statusPublished - 04-09-2018

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Enzyme activity
Acetylcholinesterase
Blood
Erythrocytes
Cells
Erythrocyte Indices
Enzymes
Anemia
Macrocytic Anemia
Hemoglobins
Hemolytic Anemia
Cholinesterases
Ethics Committees
Biochemistry
Nerve Endings
Blood Sedimentation
Hydrolases
Sedimentation
Membranes

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Gupta, Shalvika ; Belle, Vijetha Shenoy ; Kumbarakeri Rajashekhar, Ramya ; Jogi, Sushma ; Prabhu, RV Krishnananda. / Correlation of Red Blood Cell Acetylcholinesterase Enzyme Activity with Various RBC Indices. In: Indian Journal of Clinical Biochemistry. 2018 ; pp. 1-5.
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abstract = "Cholinesterases belongs to class hydrolases. There are two types acetylcholinesterase and butyryl cholinesterase. Acetylcholinesterase present in nerve endings and also in the RBC membrane. It helps to maintain the shape and size of RBCs. Any change in shape and size of RBCs may affect the activity of Acetylcholinesterase. Thus this study aimed to estimate RBCs Acetylcholinesterase enzyme activity in various types of anemias and correlate the RBCs Acetylcholinesterase enzyme activity with various hematological indices such as Erythrocyte Sedimentation Rate (ESR), Mean Corpuscular Hemoglobin (MCH), Mean Corpuscular Hemoglobin Concentration (MCHC), Mean Corpuscular Volume (MCV), Red cell Distribution Width (RDW) etc. After obtaining ethical approval from Institutional ethics committee total of 100 samples were collected from Clinical Biochemistry laboratory, Kasturba Medical College, Manipal, Manipal University. 25 were having normal RBC indices, 12 with hemolytic anemia, 26 with microcytic anemia and 26 with macrocytic anemia based on peripheral smear report and RBC indices. Acetylcholinesterase were measured using Ellman’s method. RBC acetylcholinesterase activity was significantly increased in microcytic anemia (58.13 ± 5.4) and macrocytic anemia (76.87 ± 6.7) than normal group (37.62 ± 2.71). Also increased RBC acetylcholinesterase was seen in hemolytic anemia (48.11 ± 5.18) but the increase is not statistically significant. RBC acetylcholinesterase correlated negatively with hemoglobin (r = −0.356, p = 0.001) and positively with RDW (r = 0.31, p = 0.003). To conclude RBC acetylcholinesterase activity can be used as one of the potential marker for various types of anemia.",
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Correlation of Red Blood Cell Acetylcholinesterase Enzyme Activity with Various RBC Indices. / Gupta, Shalvika; Belle, Vijetha Shenoy; Kumbarakeri Rajashekhar, Ramya; Jogi, Sushma; Prabhu, RV Krishnananda.

In: Indian Journal of Clinical Biochemistry, 04.09.2018, p. 1-5.

Research output: Contribution to journalArticle

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