CpG methylation of an endogenous retroviral enhancer inhibits transcription factor binding and activity.

B. T. Lamb, K. Satyamoorthy, L. Li, D. Solter, C. C. Howe

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    The endogenous retrovirus, intracisternal A-particle (IAP), is expressed at unique stages during murine embryogenesis and is also activated during the in vitro differentiation of F9 cells. We have examined the DNA elements and protein factors that control IAP expression during F9 differentiation. In the present study an IAP upstream enhancer (IUE) is identified by transient transfection assays and found to be active in both undifferentiated and differentiated cells. Further analyses reveal that a ubiquitous 65 kDa protein factor, the IUE binding protein (IUEB), binds with the IUE. Site-specific methylation within the IUEB binding site strongly inhibits both IUEB binding and IUE transcriptional activity, suggesting that methylation may regulate IUE function and IAP expression.

    Original languageEnglish
    Pages (from-to)185-196
    Number of pages12
    JournalGene Expression
    Volume1
    Issue number3
    Publication statusPublished - 1991

    Fingerprint

    Methylation
    Carrier Proteins
    Transcription Factors
    Protein Binding
    Endogenous Retroviruses
    Embryonic Development
    Transfection
    Cell Differentiation
    Proteins
    Binding Sites
    DNA
    In Vitro Techniques

    All Science Journal Classification (ASJC) codes

    • Genetics

    Cite this

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    abstract = "The endogenous retrovirus, intracisternal A-particle (IAP), is expressed at unique stages during murine embryogenesis and is also activated during the in vitro differentiation of F9 cells. We have examined the DNA elements and protein factors that control IAP expression during F9 differentiation. In the present study an IAP upstream enhancer (IUE) is identified by transient transfection assays and found to be active in both undifferentiated and differentiated cells. Further analyses reveal that a ubiquitous 65 kDa protein factor, the IUE binding protein (IUEB), binds with the IUE. Site-specific methylation within the IUEB binding site strongly inhibits both IUEB binding and IUE transcriptional activity, suggesting that methylation may regulate IUE function and IAP expression.",
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    CpG methylation of an endogenous retroviral enhancer inhibits transcription factor binding and activity. / Lamb, B. T.; Satyamoorthy, K.; Li, L.; Solter, D.; Howe, C. C.

    In: Gene Expression, Vol. 1, No. 3, 1991, p. 185-196.

    Research output: Contribution to journalArticle

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    AU - Lamb, B. T.

    AU - Satyamoorthy, K.

    AU - Li, L.

    AU - Solter, D.

    AU - Howe, C. C.

    PY - 1991

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