Crosslinking of gelatin-based drug carriers by genipin induces changes in drug kinetic profiles in vitro

Goutam Thakur, Analava Mitra, Dérick Rousseau, Amit Basak, Siddik Sarkar, Kunal Pal

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Abstract

Hydrogels are extensively studied as carrier matrices for the controlled release of bioactive molecules. The aim of this study was to design gelatin-based hydrogels crosslinked with genipin and study the impact of crosslinking temperature (5, 15 or 25°C) on gel strength, microstructure, cytocompatibility, swelling and drug release. Gels crosslinked at 25°C exhibited the highest Flory-Rehner crosslink density, lowest swelling ratio and the slowest release of indomethacin (Idn, model anti-inflammatory drug). Diffusional exponents (n) indicated non-Fickian swelling kinetics while drug transport was anomalous. Hydrogel biocompatibility, in vitro cell viability, cell cycle experiments with AH-927 and HaCaT cell lines indicated normal cell proliferation without any effect on cell cycle. Overall, these results substantiated the use of genipin-crosslinked hydrogels as a viable carrier matrix for drug release applications.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalJournal of Materials Science: Materials in Medicine
Volume22
Issue number1
DOIs
Publication statusPublished - 01-01-2011

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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