Curcumin half analog modulates Interleukin-6 and tumor necrosis factor-alpha in inflammatory bowel disease

Phani Krishna Kondamudi, Hemalatha Kovelamudi, Pawan G. Nayak, Mallikarjuna Chamallamudi Rao, Rekha Raghuveer Shenoy

Research output: Contribution to journalArticle

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Abstract

Background: The present study was aimed at examining the effect of dehydrozingerone (DHZ), half analogue of curcumin which is the active constituent of turmeric (Curcuma longa) in the di-nitrochlorobenzene (DNCB) induced model for inflammatory bowel disease (IBD). Materials and Methods: Male Wistar rats (200-220 g) were divided into four groups (n = 6). Chemical induction of IBD was done by sensitizing with 300 μL of 20 g/L of DNCB (in acetone) onto the nape of rats for 14 days followed by intra-colonic instillation of 250 μL of DNCB (0.1% DNCB in 50% alcohol) solution on day 15. Rats in Group 1 (normal control) and Group 2 (DNCB control) were treated with vehicle. Rats in Group 3 were treated with DHZ (100 mg/kg, p.o.; 8 days) and Group 4 animals were treated with sulfasalazine (SS) (100 mg/kg, p.o.; 8 days). On 24th day, the rats were killed, colon removed and the macroscopic, biochemical, and histopathological evaluations were performed. Results: The levels of myeloperoxidase, thiobarbituric acid reactive substrate, and nitrite increased significantly (P < 0.05) in the DNCB group whereas reduced significantly in the DHZ and SS treated groups. Serum nitrite levels were found to be insignificant between the different groups. Interleukin-6, tumor necrosis factor-alpha level was significantly high in the DNCB group. Conclusion: These findings show that DHZ can be a promising molecule for the treatment of IBD.

Original languageEnglish
Pages (from-to)S296-S302
JournalPharmacognosy Magazine
Volume11
Issue number44
DOIs
Publication statusPublished - 2015

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Curcumin
Inflammatory Bowel Diseases
Interleukin-6
Tumor Necrosis Factor-alpha
Curcuma
Sulfasalazine
Nitrites
Acetone
Peroxidase
Wistar Rats
Colon
Alcohols
Control Groups
methyl-3-methoxy-4-hydroxystyryl ketone
Serum

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery

Cite this

@article{2f6d642112734e7a8d528c9fd9dee1f6,
title = "Curcumin half analog modulates Interleukin-6 and tumor necrosis factor-alpha in inflammatory bowel disease",
abstract = "Background: The present study was aimed at examining the effect of dehydrozingerone (DHZ), half analogue of curcumin which is the active constituent of turmeric (Curcuma longa) in the di-nitrochlorobenzene (DNCB) induced model for inflammatory bowel disease (IBD). Materials and Methods: Male Wistar rats (200-220 g) were divided into four groups (n = 6). Chemical induction of IBD was done by sensitizing with 300 μL of 20 g/L of DNCB (in acetone) onto the nape of rats for 14 days followed by intra-colonic instillation of 250 μL of DNCB (0.1{\%} DNCB in 50{\%} alcohol) solution on day 15. Rats in Group 1 (normal control) and Group 2 (DNCB control) were treated with vehicle. Rats in Group 3 were treated with DHZ (100 mg/kg, p.o.; 8 days) and Group 4 animals were treated with sulfasalazine (SS) (100 mg/kg, p.o.; 8 days). On 24th day, the rats were killed, colon removed and the macroscopic, biochemical, and histopathological evaluations were performed. Results: The levels of myeloperoxidase, thiobarbituric acid reactive substrate, and nitrite increased significantly (P < 0.05) in the DNCB group whereas reduced significantly in the DHZ and SS treated groups. Serum nitrite levels were found to be insignificant between the different groups. Interleukin-6, tumor necrosis factor-alpha level was significantly high in the DNCB group. Conclusion: These findings show that DHZ can be a promising molecule for the treatment of IBD.",
author = "Kondamudi, {Phani Krishna} and Hemalatha Kovelamudi and Nayak, {Pawan G.} and Rao, {Mallikarjuna Chamallamudi} and Shenoy, {Rekha Raghuveer}",
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Curcumin half analog modulates Interleukin-6 and tumor necrosis factor-alpha in inflammatory bowel disease. / Kondamudi, Phani Krishna; Kovelamudi, Hemalatha; Nayak, Pawan G.; Rao, Mallikarjuna Chamallamudi; Shenoy, Rekha Raghuveer.

In: Pharmacognosy Magazine, Vol. 11, No. 44, 2015, p. S296-S302.

Research output: Contribution to journalArticle

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T1 - Curcumin half analog modulates Interleukin-6 and tumor necrosis factor-alpha in inflammatory bowel disease

AU - Kondamudi, Phani Krishna

AU - Kovelamudi, Hemalatha

AU - Nayak, Pawan G.

AU - Rao, Mallikarjuna Chamallamudi

AU - Shenoy, Rekha Raghuveer

PY - 2015

Y1 - 2015

N2 - Background: The present study was aimed at examining the effect of dehydrozingerone (DHZ), half analogue of curcumin which is the active constituent of turmeric (Curcuma longa) in the di-nitrochlorobenzene (DNCB) induced model for inflammatory bowel disease (IBD). Materials and Methods: Male Wistar rats (200-220 g) were divided into four groups (n = 6). Chemical induction of IBD was done by sensitizing with 300 μL of 20 g/L of DNCB (in acetone) onto the nape of rats for 14 days followed by intra-colonic instillation of 250 μL of DNCB (0.1% DNCB in 50% alcohol) solution on day 15. Rats in Group 1 (normal control) and Group 2 (DNCB control) were treated with vehicle. Rats in Group 3 were treated with DHZ (100 mg/kg, p.o.; 8 days) and Group 4 animals were treated with sulfasalazine (SS) (100 mg/kg, p.o.; 8 days). On 24th day, the rats were killed, colon removed and the macroscopic, biochemical, and histopathological evaluations were performed. Results: The levels of myeloperoxidase, thiobarbituric acid reactive substrate, and nitrite increased significantly (P < 0.05) in the DNCB group whereas reduced significantly in the DHZ and SS treated groups. Serum nitrite levels were found to be insignificant between the different groups. Interleukin-6, tumor necrosis factor-alpha level was significantly high in the DNCB group. Conclusion: These findings show that DHZ can be a promising molecule for the treatment of IBD.

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