Curcumin inhibits telomerase and induces telomere shortening and apoptosis in brain tumour cells

M. Prakash Hande, Aik Kia Khaw, M. Pradeepa Hande, Guruprasad Kalthur

Research output: Contribution to journalArticle

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Abstract

Curcumin, a polyphenolic compound isolated from Curcuma longa (Turmeric) is widely used in traditional Ayurvedic medicine. Its potential therapeutic effects on a variety of diseases have long been known. Though anti-tumour effects of curcumin have been reported earlier, its mode of action and telomerase inhibitory effects are not clearly determined in brain tumour cells. In the present study, we demonstrate that curcumin binds to cell surface membrane and infiltrates into cytoplasm to initiate apoptotic events. Curcumin treatment has resulted in higher cytotoxicity in the cells that express telomerase enzyme, highlighting its potential as an anticancer agent. Curcumin induced growth inhibition and cell cycle arrest at G2/M phase in the glioblastoma and medulloblastoma cells used in the study. Gene and protein expression analyses revealed that curcumin down-regulated CCNE1, E2F1 and CDK2 and up-regulated the expression of PTEN genes resulting in growth arrest at G2/M phase. Curcumin-induced apoptosis is found to be associated with increased caspase-3/7 activity and overexpression of Bax. In addition, down-regulation of Bcl2 and survivin was observed in curcumin-treated cells. Besides these effects, we found curcumin to be inhibiting telomerase activity and down-regulating hTERT mRNA expression leading to telomere shortening. We conclude that telomerase inhibitory effects of curcumin underscore its use in adjuvant cancer therapy. J. Cell. Biochem. 114: 1257-1270, 2013. © 2012 Wiley Periodicals, Inc.

Original languageEnglish
Pages (from-to)1257-1270
Number of pages14
JournalJournal of Cellular Biochemistry
Volume114
Issue number6
DOIs
Publication statusPublished - 06-2013

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Telomere Shortening
Curcumin
Telomerase
Brain Neoplasms
Tumors
Brain
Cells
Apoptosis
Curcuma
G2 Phase
Cell Division
Polyphenolic compounds
Ayurvedic Medicine
Caspase 7
Gene Expression
Medulloblastoma
Bioelectric potentials
Traditional Medicine
Therapeutic Uses
Glioblastoma

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Hande, M. Prakash ; Khaw, Aik Kia ; Hande, M. Pradeepa ; Kalthur, Guruprasad. / Curcumin inhibits telomerase and induces telomere shortening and apoptosis in brain tumour cells. In: Journal of Cellular Biochemistry. 2013 ; Vol. 114, No. 6. pp. 1257-1270.
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Curcumin inhibits telomerase and induces telomere shortening and apoptosis in brain tumour cells. / Hande, M. Prakash; Khaw, Aik Kia; Hande, M. Pradeepa; Kalthur, Guruprasad.

In: Journal of Cellular Biochemistry, Vol. 114, No. 6, 06.2013, p. 1257-1270.

Research output: Contribution to journalArticle

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