Cytotoxic effect of Coscinium fenestratum (Gaertn.) Colebr. and its active principle berberine on L929 cells

S. Narasimhan, G. M. Nair

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Coscinium fenestratum (Gaertn.) Colebr. is a medicinal liana found in the Western Ghats of Southern India, Sri Lanka, Cambodia and Malaysia. Water and methanol extracts prepared from the stem of C. fenestratum were evaluated for cytotoxicity on L929 fibroblast cells. Berberine taken for the cytotoxic evaluation was obtained through cell suspension cultures established from young green leaf segments. MTT (3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H tetrazolium bromide) assay was performed to evaluate the in vitro cytotoxicity. Berberine present in the water and methanol extract was determined by RP-HPLC. Methanol extracts demonstrated better inhibitory effects on cultured L929 cells followed by purified berberine from cell suspension cultures and water extracts. Only 15.33% of the cells was metabolically active at 200 μg/ml methanol extract while 23.57% of the cells was active at 200 μg/ml berberine. Increased inhibitory effect of methanol extracts over berberine demonstrates the occurrence of other cytotoxic principles along with main active principle berberine or other factors enhancing the cytotoxic effect of berberine.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalMedicinal Chemistry Research
Volume14
Issue number2
DOIs
Publication statusPublished - 01-12-2005

Fingerprint

Berberine
Methanol
Cytotoxicity
Water
Suspensions
Cell Culture Techniques
Cambodia
Sri Lanka
Malaysia
Fibroblasts
Bromides
India
Cultured Cells
Assays
High Pressure Liquid Chromatography
Cells

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Organic Chemistry

Cite this

@article{8de8a6d1adc1426696ee0880c87fbd32,
title = "Cytotoxic effect of Coscinium fenestratum (Gaertn.) Colebr. and its active principle berberine on L929 cells",
abstract = "Coscinium fenestratum (Gaertn.) Colebr. is a medicinal liana found in the Western Ghats of Southern India, Sri Lanka, Cambodia and Malaysia. Water and methanol extracts prepared from the stem of C. fenestratum were evaluated for cytotoxicity on L929 fibroblast cells. Berberine taken for the cytotoxic evaluation was obtained through cell suspension cultures established from young green leaf segments. MTT (3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H tetrazolium bromide) assay was performed to evaluate the in vitro cytotoxicity. Berberine present in the water and methanol extract was determined by RP-HPLC. Methanol extracts demonstrated better inhibitory effects on cultured L929 cells followed by purified berberine from cell suspension cultures and water extracts. Only 15.33{\%} of the cells was metabolically active at 200 μg/ml methanol extract while 23.57{\%} of the cells was active at 200 μg/ml berberine. Increased inhibitory effect of methanol extracts over berberine demonstrates the occurrence of other cytotoxic principles along with main active principle berberine or other factors enhancing the cytotoxic effect of berberine.",
author = "S. Narasimhan and Nair, {G. M.}",
year = "2005",
month = "12",
day = "1",
doi = "10.1007/s00044-005-0128-9",
language = "English",
volume = "14",
pages = "118--124",
journal = "Medicinal Chemistry Research",
issn = "1054-2523",
publisher = "Birkhause Boston",
number = "2",

}

Cytotoxic effect of Coscinium fenestratum (Gaertn.) Colebr. and its active principle berberine on L929 cells. / Narasimhan, S.; Nair, G. M.

In: Medicinal Chemistry Research, Vol. 14, No. 2, 01.12.2005, p. 118-124.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cytotoxic effect of Coscinium fenestratum (Gaertn.) Colebr. and its active principle berberine on L929 cells

AU - Narasimhan, S.

AU - Nair, G. M.

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Coscinium fenestratum (Gaertn.) Colebr. is a medicinal liana found in the Western Ghats of Southern India, Sri Lanka, Cambodia and Malaysia. Water and methanol extracts prepared from the stem of C. fenestratum were evaluated for cytotoxicity on L929 fibroblast cells. Berberine taken for the cytotoxic evaluation was obtained through cell suspension cultures established from young green leaf segments. MTT (3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H tetrazolium bromide) assay was performed to evaluate the in vitro cytotoxicity. Berberine present in the water and methanol extract was determined by RP-HPLC. Methanol extracts demonstrated better inhibitory effects on cultured L929 cells followed by purified berberine from cell suspension cultures and water extracts. Only 15.33% of the cells was metabolically active at 200 μg/ml methanol extract while 23.57% of the cells was active at 200 μg/ml berberine. Increased inhibitory effect of methanol extracts over berberine demonstrates the occurrence of other cytotoxic principles along with main active principle berberine or other factors enhancing the cytotoxic effect of berberine.

AB - Coscinium fenestratum (Gaertn.) Colebr. is a medicinal liana found in the Western Ghats of Southern India, Sri Lanka, Cambodia and Malaysia. Water and methanol extracts prepared from the stem of C. fenestratum were evaluated for cytotoxicity on L929 fibroblast cells. Berberine taken for the cytotoxic evaluation was obtained through cell suspension cultures established from young green leaf segments. MTT (3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H tetrazolium bromide) assay was performed to evaluate the in vitro cytotoxicity. Berberine present in the water and methanol extract was determined by RP-HPLC. Methanol extracts demonstrated better inhibitory effects on cultured L929 cells followed by purified berberine from cell suspension cultures and water extracts. Only 15.33% of the cells was metabolically active at 200 μg/ml methanol extract while 23.57% of the cells was active at 200 μg/ml berberine. Increased inhibitory effect of methanol extracts over berberine demonstrates the occurrence of other cytotoxic principles along with main active principle berberine or other factors enhancing the cytotoxic effect of berberine.

UR - http://www.scopus.com/inward/record.url?scp=31944442497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31944442497&partnerID=8YFLogxK

U2 - 10.1007/s00044-005-0128-9

DO - 10.1007/s00044-005-0128-9

M3 - Article

VL - 14

SP - 118

EP - 124

JO - Medicinal Chemistry Research

JF - Medicinal Chemistry Research

SN - 1054-2523

IS - 2

ER -