Cytotoxic, genotoxic and oxidative stress induced by 1,4-naphthoquinone in B16F1 melanoma tumor cells

Mandala Rayabandla Sunil Kumar, Kiran Aithal, Bhuvanagiri Nageshwar Rao, Nayanabhirama Udupa, Bola Sadashiva Satish Rao

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Quinones have diverse pharmacological properties including antibacterial, antifungal, antiviral, anti-inflammatory, antipyretic and anticancer activity. The cytotoxic potential of 1,4-naphthoquinone (NQ14) was studied against B16F1 melanoma cells grown in vitro. NQ14 treatment resulted in a concentration-dependent cytotoxicity as indicated by MTT assay and lactate dehydrogenase leakage assay. Depletion in cellular glutathione levels after 1 h incubation with NQ14 correlated with the corresponding increase in reactive oxygen species generation as determined by 2′,7′-dicholorofluorescein diacetate assay suggests the role of oxidative stress in cell death. The frequency of micronucleated binucleate cells increased with increasing doses of NQ14 with a corresponding decrease in the cytokinesis block proliferation index indicating the drug induced genotoxicity and cell division delay. Further, a dose-dependent decrease in the clonogenic cell survival indicated the potential of NQ14 to inhibit cell proliferation contributing to cell death. The cell death after NQ14 treatment may be attributed to apoptosis as seen in DNA ladder pattern along with necrosis as indicated in flow cytometric analysis of Annexin V/PI stained cells. Results of the present study demonstrate the cytotoxic and genotoxic potential of NQ14 by the induction of oxidative stress mediated mechanisms leading to tumor cell kill.

Original languageEnglish
Pages (from-to)242-250
Number of pages9
JournalToxicology in Vitro
Volume23
Issue number2
DOIs
Publication statusPublished - 03-2009

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Oxidative stress
DNA Damage
Tumors
Melanoma
Oxidative Stress
Cell death
Cells
Assays
Cell Death
Neoplasms
Quinones
Antipyretics
Cytokinesis
Annexin A5
Ladders
Cell proliferation
Cytotoxicity
L-Lactate Dehydrogenase
Cell Division
Antiviral Agents

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Kumar, Mandala Rayabandla Sunil ; Aithal, Kiran ; Rao, Bhuvanagiri Nageshwar ; Udupa, Nayanabhirama ; Rao, Bola Sadashiva Satish. / Cytotoxic, genotoxic and oxidative stress induced by 1,4-naphthoquinone in B16F1 melanoma tumor cells. In: Toxicology in Vitro. 2009 ; Vol. 23, No. 2. pp. 242-250.
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Cytotoxic, genotoxic and oxidative stress induced by 1,4-naphthoquinone in B16F1 melanoma tumor cells. / Kumar, Mandala Rayabandla Sunil; Aithal, Kiran; Rao, Bhuvanagiri Nageshwar; Udupa, Nayanabhirama; Rao, Bola Sadashiva Satish.

In: Toxicology in Vitro, Vol. 23, No. 2, 03.2009, p. 242-250.

Research output: Contribution to journalArticle

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