Cytotoxicity studies of microwave assisted natural product extracts in hela and MCF-7 cell lines

Sunil Dhanya, N. V.Anil Kumar, Anand Shivaram Nayak, U. Sachin Raj, Deepa Prabhu, Ravikiran

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Microwave assisted extraction of leaves and stems of seven tropical plants viz. Ficus hispida, Andrographis paniculata, Alistonia scholaris, Aporusa lindlyeyana, Melastoma malabathicum, Adenon indicum and Alternanthera dentata was carried out. Optimum extraction conditions were 600 W microwave power, 6 min extraction time (spread over two extraction cycles of 3 min each) and 10 min pre-leaching time using powdered stem and leaves samples. The two solvents employed were 90% ethanol and hexane with 10:1 (mL/g) as the solvent to material loading ratio. The residues obtained were screened for their cytotoxic activity by MTT assay in HeLa and MCF-7 cell lines. The ethanol extract of F. hispida (Moraceae) and A. scholaris (Apocyanaceae) leaves exhibited excellent cytotoxic activity with an IC50 value of 8.47μg/mL in HeLa and 10.23 μg/mL in MCF-7 cell lines, respectively. The alcoholic leaf extract of A. sholaris showed IC50 values of 20.62 μg/mL and 18.50 μg/mL in HeLa and MCF-7 cells respectively.

Original languageEnglish
Pages (from-to)27-31
Number of pages5
JournalInternational Journal of Medicinal and Aromatic Plants
Volume3
Issue number1
Publication statusPublished - 2013

Fingerprint

MCF-7 Cells
Microwaves
Biological Products
cytotoxicity
Ficus hispida
cell lines
Cell Line
Inhibitory Concentration 50
extracts
Ethanol
Andrographis
Moraceae
Ficus
inhibitory concentration 50
Melastoma
Alternanthera
ethanol
Hexanes
Andrographis paniculata
leaves

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Drug Discovery

Cite this

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title = "Cytotoxicity studies of microwave assisted natural product extracts in hela and MCF-7 cell lines",
abstract = "Microwave assisted extraction of leaves and stems of seven tropical plants viz. Ficus hispida, Andrographis paniculata, Alistonia scholaris, Aporusa lindlyeyana, Melastoma malabathicum, Adenon indicum and Alternanthera dentata was carried out. Optimum extraction conditions were 600 W microwave power, 6 min extraction time (spread over two extraction cycles of 3 min each) and 10 min pre-leaching time using powdered stem and leaves samples. The two solvents employed were 90{\%} ethanol and hexane with 10:1 (mL/g) as the solvent to material loading ratio. The residues obtained were screened for their cytotoxic activity by MTT assay in HeLa and MCF-7 cell lines. The ethanol extract of F. hispida (Moraceae) and A. scholaris (Apocyanaceae) leaves exhibited excellent cytotoxic activity with an IC50 value of 8.47μg/mL in HeLa and 10.23 μg/mL in MCF-7 cell lines, respectively. The alcoholic leaf extract of A. sholaris showed IC50 values of 20.62 μg/mL and 18.50 μg/mL in HeLa and MCF-7 cells respectively.",
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Cytotoxicity studies of microwave assisted natural product extracts in hela and MCF-7 cell lines. / Dhanya, Sunil; Kumar, N. V.Anil; Nayak, Anand Shivaram; Raj, U. Sachin; Prabhu, Deepa; Ravikiran.

In: International Journal of Medicinal and Aromatic Plants, Vol. 3, No. 1, 2013, p. 27-31.

Research output: Contribution to journalArticle

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AU - Dhanya, Sunil

AU - Kumar, N. V.Anil

AU - Nayak, Anand Shivaram

AU - Raj, U. Sachin

AU - Prabhu, Deepa

AU - Ravikiran,

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Y1 - 2013

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