Dental pulp stem cells from primary teeth quality analysis: Laboratory procedures

Rooposhi Saha, Shobha Tandon, Ramesh Rajendran, Rashmi Nayak

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Objectives: To present details of isolation, processing and differentiation of stem cells from inflamed dental pulp of primary teeth. Materials and methods: Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow. Results: There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages. Conclusion: Isolation of cells from oral tissues is technique sensitive.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalJournal of Clinical Pediatric Dentistry
Volume36
Issue number2
DOIs
Publication statusPublished - 01-01-2011

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Dentistry(all)

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