TY - JOUR
T1 - Dental pulp stem cells from primary teeth quality analysis
T2 - Laboratory procedures
AU - Saha, Rooposhi
AU - Tandon, Shobha
AU - Rajendran, Ramesh
AU - Nayak, Rashmi
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Objectives: To present details of isolation, processing and differentiation of stem cells from inflamed dental pulp of primary teeth. Materials and methods: Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow. Results: There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages. Conclusion: Isolation of cells from oral tissues is technique sensitive.
AB - Objectives: To present details of isolation, processing and differentiation of stem cells from inflamed dental pulp of primary teeth. Materials and methods: Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow. Results: There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages. Conclusion: Isolation of cells from oral tissues is technique sensitive.
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U2 - 10.17796/jcpd.36.2.6j0w230354732454
DO - 10.17796/jcpd.36.2.6j0w230354732454
M3 - Article
C2 - 22524079
AN - SCOPUS:84860624609
SN - 1053-4628
VL - 36
SP - 167
EP - 173
JO - Journal of Clinical Pediatric Dentistry
JF - Journal of Clinical Pediatric Dentistry
IS - 2
ER -