Detection of genital HPV infection using urine samples

A population based study in India

Sasidharanpillai Sabeena, Parvati Bhat, Veena Kamath, Mary Mathew, Sushama Aswathyraj, Santhosha Devadiga, Suresha Prabhu, Maity Hindol, Akhil Chameetachal, Anjana Krishnan, Govindakarnavar Arunkumar

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Cervical cancer is the second commonest cancer among Indian women and its association with human papilloma virus (HPV) is well established. This preventable cancer accounts for the maximum number of cancer related deaths among rural Indian women. Unlike in developed countries there are no organized cervical cancer screening programmes in India due to lack of resources and manpower. Objective: To detect genital HPV infection using urine samples among asymptomatic rural women in the age group of 18-65 years. Materials and Methods: The study area chosen was Perdoor village in Udupi Taluk, Karnataka State and all the women in the age group of 18-65 years formed the study cohort. A cross sectional study was conducted by house visits and 1,305 women were enrolled in the study. After taking written informed consent a data sheet was filled and early stream random urine samples were collected, transported to a laboratory at 4°C and aliquoted. Samples were tested using nested HPV PCR with PGMY09/11 and GP5+/6+ primers. Positive cases were genotyped by sequence analysis. Results: Study participants included 1,134 sexually active and 171 unmarried women with a mean age at marriage of 22.1 (SD=3.9) years. Study area showed high female literacy rate of 86.6%. Five urine samples tested positive for HPV DNA (0.4%). Conclusions: We found very low genital HPV infection rate among women from monogamous community. This is the first major population based study carried out among asymptomatic rural women to detect genital HPV infectio from Karnataka using urine samples.

Original languageEnglish
Pages (from-to)1083-1088
Number of pages6
JournalAsian Pacific Journal of Cancer Prevention
Volume17
Issue number3
DOIs
Publication statusPublished - 19-04-2016

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Papillomaviridae
Virus Diseases
India
Urine
Population
Uterine Cervical Neoplasms
Age Groups
Second Primary Neoplasms
Marriage
Informed Consent
Early Detection of Cancer
Developed Countries
Sequence Analysis
Neoplasms
Cohort Studies

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Sabeena, Sasidharanpillai ; Bhat, Parvati ; Kamath, Veena ; Mathew, Mary ; Aswathyraj, Sushama ; Devadiga, Santhosha ; Prabhu, Suresha ; Hindol, Maity ; Chameetachal, Akhil ; Krishnan, Anjana ; Arunkumar, Govindakarnavar. / Detection of genital HPV infection using urine samples : A population based study in India. In: Asian Pacific Journal of Cancer Prevention. 2016 ; Vol. 17, No. 3. pp. 1083-1088.
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Detection of genital HPV infection using urine samples : A population based study in India. / Sabeena, Sasidharanpillai; Bhat, Parvati; Kamath, Veena; Mathew, Mary; Aswathyraj, Sushama; Devadiga, Santhosha; Prabhu, Suresha; Hindol, Maity; Chameetachal, Akhil; Krishnan, Anjana; Arunkumar, Govindakarnavar.

In: Asian Pacific Journal of Cancer Prevention, Vol. 17, No. 3, 19.04.2016, p. 1083-1088.

Research output: Contribution to journalArticle

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T1 - Detection of genital HPV infection using urine samples

T2 - A population based study in India

AU - Sabeena, Sasidharanpillai

AU - Bhat, Parvati

AU - Kamath, Veena

AU - Mathew, Mary

AU - Aswathyraj, Sushama

AU - Devadiga, Santhosha

AU - Prabhu, Suresha

AU - Hindol, Maity

AU - Chameetachal, Akhil

AU - Krishnan, Anjana

AU - Arunkumar, Govindakarnavar

PY - 2016/4/19

Y1 - 2016/4/19

N2 - Background: Cervical cancer is the second commonest cancer among Indian women and its association with human papilloma virus (HPV) is well established. This preventable cancer accounts for the maximum number of cancer related deaths among rural Indian women. Unlike in developed countries there are no organized cervical cancer screening programmes in India due to lack of resources and manpower. Objective: To detect genital HPV infection using urine samples among asymptomatic rural women in the age group of 18-65 years. Materials and Methods: The study area chosen was Perdoor village in Udupi Taluk, Karnataka State and all the women in the age group of 18-65 years formed the study cohort. A cross sectional study was conducted by house visits and 1,305 women were enrolled in the study. After taking written informed consent a data sheet was filled and early stream random urine samples were collected, transported to a laboratory at 4°C and aliquoted. Samples were tested using nested HPV PCR with PGMY09/11 and GP5+/6+ primers. Positive cases were genotyped by sequence analysis. Results: Study participants included 1,134 sexually active and 171 unmarried women with a mean age at marriage of 22.1 (SD=3.9) years. Study area showed high female literacy rate of 86.6%. Five urine samples tested positive for HPV DNA (0.4%). Conclusions: We found very low genital HPV infection rate among women from monogamous community. This is the first major population based study carried out among asymptomatic rural women to detect genital HPV infectio from Karnataka using urine samples.

AB - Background: Cervical cancer is the second commonest cancer among Indian women and its association with human papilloma virus (HPV) is well established. This preventable cancer accounts for the maximum number of cancer related deaths among rural Indian women. Unlike in developed countries there are no organized cervical cancer screening programmes in India due to lack of resources and manpower. Objective: To detect genital HPV infection using urine samples among asymptomatic rural women in the age group of 18-65 years. Materials and Methods: The study area chosen was Perdoor village in Udupi Taluk, Karnataka State and all the women in the age group of 18-65 years formed the study cohort. A cross sectional study was conducted by house visits and 1,305 women were enrolled in the study. After taking written informed consent a data sheet was filled and early stream random urine samples were collected, transported to a laboratory at 4°C and aliquoted. Samples were tested using nested HPV PCR with PGMY09/11 and GP5+/6+ primers. Positive cases were genotyped by sequence analysis. Results: Study participants included 1,134 sexually active and 171 unmarried women with a mean age at marriage of 22.1 (SD=3.9) years. Study area showed high female literacy rate of 86.6%. Five urine samples tested positive for HPV DNA (0.4%). Conclusions: We found very low genital HPV infection rate among women from monogamous community. This is the first major population based study carried out among asymptomatic rural women to detect genital HPV infectio from Karnataka using urine samples.

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