Detection of Helicobactor pylori by polymerase chain reaction

A comparison in sample preparation

R. Murugesan, P. Bhattacharjee, P. R.Vivek Kumar, Mary N. Mohankumar, Mangala Nagarajan, R. K. Jeevanram

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Gastric biopsy samples obtained from 14 patients with upper abdominal pain, clinically diagnosed as acid peptic disease, were analysed for the presence of Helicobacter pylori (H. pylori) by Polymerase Chain Reaction (PCR) using partially (template A) and completely purified DNA (template B). Antigen specific primer was used to analyse the sample by PCR method. The presence of H. pylori in the samples was confirmed by running a positive control. The presence of H. pylori was also detected by urease method using standard protocol. Among the 14 samples studied, 8 showed the presence of H. pylori with both templates A and B. Among these 8 samples only 3 showed positive for the presence of H. pylori with urease method. The present work discusses the results obtained in the detection of H. pylori in template A and B by PCR method.

Original languageEnglish
Pages (from-to)35-38
Number of pages4
JournalIndian Journal of Human Genetics
Volume11
Issue number1
Publication statusPublished - 01-01-2005
Externally publishedYes

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Pylorus
Helicobacter pylori
Polymerase Chain Reaction
Urease
Abdominal Pain
Digestion
Stomach
Biopsy
Antigens
Acids
DNA

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Murugesan, R., Bhattacharjee, P., Kumar, P. R. V., Mohankumar, M. N., Nagarajan, M., & Jeevanram, R. K. (2005). Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation. Indian Journal of Human Genetics, 11(1), 35-38.
Murugesan, R. ; Bhattacharjee, P. ; Kumar, P. R.Vivek ; Mohankumar, Mary N. ; Nagarajan, Mangala ; Jeevanram, R. K. / Detection of Helicobactor pylori by polymerase chain reaction : A comparison in sample preparation. In: Indian Journal of Human Genetics. 2005 ; Vol. 11, No. 1. pp. 35-38.
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Murugesan, R, Bhattacharjee, P, Kumar, PRV, Mohankumar, MN, Nagarajan, M & Jeevanram, RK 2005, 'Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation', Indian Journal of Human Genetics, vol. 11, no. 1, pp. 35-38.

Detection of Helicobactor pylori by polymerase chain reaction : A comparison in sample preparation. / Murugesan, R.; Bhattacharjee, P.; Kumar, P. R.Vivek; Mohankumar, Mary N.; Nagarajan, Mangala; Jeevanram, R. K.

In: Indian Journal of Human Genetics, Vol. 11, No. 1, 01.01.2005, p. 35-38.

Research output: Contribution to journalArticle

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Murugesan R, Bhattacharjee P, Kumar PRV, Mohankumar MN, Nagarajan M, Jeevanram RK. Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation. Indian Journal of Human Genetics. 2005 Jan 1;11(1):35-38.