Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation

R. Murugesan, P. Bhattacharjee, P. R.Vivek Kumar, Mary N. Mohankumar, Mangala Nagarajan, R. K. Jeevanram

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Gastric biopsy samples obtained from 14 patients with upper abdominal pain, clinically diagnosed as acid peptic disease, were analysed for the presence of Helicobacter pylori (H. pylori) by Polymerase Chain Reaction (PCR) using partially (template A) and completely purified DNA (template B). Antigen specific primer was used to analyse the sample by PCR method. The presence of H. pylori in the samples was confirmed by running a positive control. The presence of H. pylori was also detected by urease method using standard protocol. Among the 14 samples studied, 8 showed the presence of H. pylori with both templates A and B. Among these 8 samples only 3 showed positive for the presence of H. pylori with urease method. The present work discusses the results obtained in the detection of H. pylori in template A and B by PCR method.

Original languageEnglish
Pages (from-to)35-38
Number of pages4
JournalIndian Journal of Human Genetics
Volume11
Issue number1
Publication statusPublished - 01-01-2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation'. Together they form a unique fingerprint.

  • Cite this

    Murugesan, R., Bhattacharjee, P., Kumar, P. R. V., Mohankumar, M. N., Nagarajan, M., & Jeevanram, R. K. (2005). Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation. Indian Journal of Human Genetics, 11(1), 35-38.