Determination of racecadotril by HPLC in capsules

S. Prabu, T. Singh, A. Joseph, C. Kumar, A. Shirwaikar

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A simple, precise and rapid RP-HPLC method was developed for the determination of racecadotril in a pharmaceutical formulation using gemfibrozil as internal standard. Ratio of the peak area of analyte to internal standard was used for quantification. The chromatographic separation was carried out by using a Reverse Phase C18 column (BDS-Hypersil). The mobile phase consisting of a mixture of 20 mM phosphate buffer (pH 3.5) and acetonitrile in the ratio of (40:60) with detection at 230 nm at a flow rate of 1 ml/min was employed. The method was statistically validated for linearity, accuracy and precision. The elution time was 6.9 min for racecadotril and 9.8 min for gemfibrozil. The simplicity and accuracy of the proposed method ensures its use in routine quality control analysis of pharmaceutical formulations.
Original languageEnglish
Pages (from-to)819-821
Number of pages3
JournalIndian Journal of Pharmaceutical Sciences
Volume69
Issue number6
Publication statusPublished - 2007

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Gemfibrozil
Capsules
Drug Compounding
High Pressure Liquid Chromatography
Quality Control
Buffers
Phosphates
racecadotril
acetonitrile

Cite this

Prabu, S., Singh, T., Joseph, A., Kumar, C., & Shirwaikar, A. (2007). Determination of racecadotril by HPLC in capsules. Indian Journal of Pharmaceutical Sciences, 69(6), 819-821.
Prabu, S. ; Singh, T. ; Joseph, A. ; Kumar, C. ; Shirwaikar, A. / Determination of racecadotril by HPLC in capsules. In: Indian Journal of Pharmaceutical Sciences. 2007 ; Vol. 69, No. 6. pp. 819-821.
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abstract = "A simple, precise and rapid RP-HPLC method was developed for the determination of racecadotril in a pharmaceutical formulation using gemfibrozil as internal standard. Ratio of the peak area of analyte to internal standard was used for quantification. The chromatographic separation was carried out by using a Reverse Phase C18 column (BDS-Hypersil). The mobile phase consisting of a mixture of 20 mM phosphate buffer (pH 3.5) and acetonitrile in the ratio of (40:60) with detection at 230 nm at a flow rate of 1 ml/min was employed. The method was statistically validated for linearity, accuracy and precision. The elution time was 6.9 min for racecadotril and 9.8 min for gemfibrozil. The simplicity and accuracy of the proposed method ensures its use in routine quality control analysis of pharmaceutical formulations.",
author = "S. Prabu and T. Singh and A. Joseph and C. Kumar and A. Shirwaikar",
note = "Cited By :6 Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Shirwaikar, A.; Department of Pharmaceutics, Gulf Pharmacy College, Gulf Medical University, Ajman, United Arab Emirates; email: arunshirwaikar@yahoo.co.in Chemicals/CAS: acetonitrile, 75-05-8; acetorphan, 81110-73-8; gemfibrozil, 25812-30-0 Manufacturers: Reddy Research Foundation, India; Sun, India References: Matheson, A.J., Noble, S., Discussions (2000) Drugs, 59, p. 829; Cezard JP, Salazar-Lindo E. Racecadotril in acute diarrhea. Indian Pediatr 2005;42:502-3; Schwartz JC. Racecadotril, a new approach to the treatment of diarrhea. J Antimicrob Agents 2000;14:75-9UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-40749107091&partnerID=40&md5=fd47d9856692da602127fd9fc2d02c66",
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Prabu, S, Singh, T, Joseph, A, Kumar, C & Shirwaikar, A 2007, 'Determination of racecadotril by HPLC in capsules', Indian Journal of Pharmaceutical Sciences, vol. 69, no. 6, pp. 819-821.

Determination of racecadotril by HPLC in capsules. / Prabu, S.; Singh, T.; Joseph, A.; Kumar, C.; Shirwaikar, A.

In: Indian Journal of Pharmaceutical Sciences, Vol. 69, No. 6, 2007, p. 819-821.

Research output: Contribution to journalArticle

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N2 - A simple, precise and rapid RP-HPLC method was developed for the determination of racecadotril in a pharmaceutical formulation using gemfibrozil as internal standard. Ratio of the peak area of analyte to internal standard was used for quantification. The chromatographic separation was carried out by using a Reverse Phase C18 column (BDS-Hypersil). The mobile phase consisting of a mixture of 20 mM phosphate buffer (pH 3.5) and acetonitrile in the ratio of (40:60) with detection at 230 nm at a flow rate of 1 ml/min was employed. The method was statistically validated for linearity, accuracy and precision. The elution time was 6.9 min for racecadotril and 9.8 min for gemfibrozil. The simplicity and accuracy of the proposed method ensures its use in routine quality control analysis of pharmaceutical formulations.

AB - A simple, precise and rapid RP-HPLC method was developed for the determination of racecadotril in a pharmaceutical formulation using gemfibrozil as internal standard. Ratio of the peak area of analyte to internal standard was used for quantification. The chromatographic separation was carried out by using a Reverse Phase C18 column (BDS-Hypersil). The mobile phase consisting of a mixture of 20 mM phosphate buffer (pH 3.5) and acetonitrile in the ratio of (40:60) with detection at 230 nm at a flow rate of 1 ml/min was employed. The method was statistically validated for linearity, accuracy and precision. The elution time was 6.9 min for racecadotril and 9.8 min for gemfibrozil. The simplicity and accuracy of the proposed method ensures its use in routine quality control analysis of pharmaceutical formulations.

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JF - Indian Journal of Pharmaceutical Sciences

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