Determining the effect of folate diets during pregnancy and lactation on neurobehavioural changes in the adult life of offspring

Nanjundappa Vinaykumar, Ashok Kumar, Lydia S. Quadros, Lokadolalu C. Prasanna

Research output: Contribution to journalArticle

Abstract

Objectives: Animal and human studies have demonstrated that folic acid (FA) is essential for nervous system and brain development. In humans, insufficient maternal FA intake is known to cause neural tube defects, autism spectrum, and other neurodevelopmental disorders in children. The present study aimed to determine the impact of maternal FA supplementation on psychomotor skills and learning and memory functions in their adult offspring. Methods: Eighteen female Wistar rats were randomly divided into three groups. The animals were fed three different concentrations of FA from preconception to pregnancy and during lactation. The adult offspring were assessed for neurobehavioural changes and histological confirmation by hippocampal neuron quantification. Results: Neurobehavioural assessment revealed a significantly smaller number of alternations, a higher percentage bias, and a greater number of working and reference memory errors. The increased time spent in the dark compartment in the FA-supplementation group indicated deficit(s) in learning memory. Hippocampal neuron quantification revealed a higher mean number of viable neurons in the cornu ammonis (CA) region in the control group (CA1 region, 31.2 ± 3.2; CA3 region, 23.2 ± 3.2), with a distinct nucleus in both regions, and least in the FA-supplementation group (CA1 region, 24.2 ± 3.1; CA3 region, 15.2 ± 2.2). Conclusion: Results of this investigation support the possible negative effect of high levels of maternal FA supplementation during pregnancy and lactation. Such alterations potentially lead to neurobehavioural changes in the adult offspring of Wistar rats.

Original languageEnglish
JournalJournal of Taibah University Medical Sciences
DOIs
Publication statusAccepted/In press - 01-01-2019

Fingerprint

Adult Children
Folic Acid
Lactation
Diet
Pregnancy
Mothers
Neurons
Wistar Rats
Learning
Neural Tube Defects
Autistic Disorder
Short-Term Memory
Nervous System
Hippocampus
Control Groups
Brain

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{a26b3fb5fdf0458f886b4942a4ecbc04,
title = "Determining the effect of folate diets during pregnancy and lactation on neurobehavioural changes in the adult life of offspring",
abstract = "Objectives: Animal and human studies have demonstrated that folic acid (FA) is essential for nervous system and brain development. In humans, insufficient maternal FA intake is known to cause neural tube defects, autism spectrum, and other neurodevelopmental disorders in children. The present study aimed to determine the impact of maternal FA supplementation on psychomotor skills and learning and memory functions in their adult offspring. Methods: Eighteen female Wistar rats were randomly divided into three groups. The animals were fed three different concentrations of FA from preconception to pregnancy and during lactation. The adult offspring were assessed for neurobehavioural changes and histological confirmation by hippocampal neuron quantification. Results: Neurobehavioural assessment revealed a significantly smaller number of alternations, a higher percentage bias, and a greater number of working and reference memory errors. The increased time spent in the dark compartment in the FA-supplementation group indicated deficit(s) in learning memory. Hippocampal neuron quantification revealed a higher mean number of viable neurons in the cornu ammonis (CA) region in the control group (CA1 region, 31.2 ± 3.2; CA3 region, 23.2 ± 3.2), with a distinct nucleus in both regions, and least in the FA-supplementation group (CA1 region, 24.2 ± 3.1; CA3 region, 15.2 ± 2.2). Conclusion: Results of this investigation support the possible negative effect of high levels of maternal FA supplementation during pregnancy and lactation. Such alterations potentially lead to neurobehavioural changes in the adult offspring of Wistar rats.",
author = "Nanjundappa Vinaykumar and Ashok Kumar and Quadros, {Lydia S.} and Prasanna, {Lokadolalu C.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.jtumed.2019.09.009",
language = "English",
journal = "Journal of Taibah University Medical Sciences",
issn = "1658-3612",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Determining the effect of folate diets during pregnancy and lactation on neurobehavioural changes in the adult life of offspring

AU - Vinaykumar, Nanjundappa

AU - Kumar, Ashok

AU - Quadros, Lydia S.

AU - Prasanna, Lokadolalu C.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Objectives: Animal and human studies have demonstrated that folic acid (FA) is essential for nervous system and brain development. In humans, insufficient maternal FA intake is known to cause neural tube defects, autism spectrum, and other neurodevelopmental disorders in children. The present study aimed to determine the impact of maternal FA supplementation on psychomotor skills and learning and memory functions in their adult offspring. Methods: Eighteen female Wistar rats were randomly divided into three groups. The animals were fed three different concentrations of FA from preconception to pregnancy and during lactation. The adult offspring were assessed for neurobehavioural changes and histological confirmation by hippocampal neuron quantification. Results: Neurobehavioural assessment revealed a significantly smaller number of alternations, a higher percentage bias, and a greater number of working and reference memory errors. The increased time spent in the dark compartment in the FA-supplementation group indicated deficit(s) in learning memory. Hippocampal neuron quantification revealed a higher mean number of viable neurons in the cornu ammonis (CA) region in the control group (CA1 region, 31.2 ± 3.2; CA3 region, 23.2 ± 3.2), with a distinct nucleus in both regions, and least in the FA-supplementation group (CA1 region, 24.2 ± 3.1; CA3 region, 15.2 ± 2.2). Conclusion: Results of this investigation support the possible negative effect of high levels of maternal FA supplementation during pregnancy and lactation. Such alterations potentially lead to neurobehavioural changes in the adult offspring of Wistar rats.

AB - Objectives: Animal and human studies have demonstrated that folic acid (FA) is essential for nervous system and brain development. In humans, insufficient maternal FA intake is known to cause neural tube defects, autism spectrum, and other neurodevelopmental disorders in children. The present study aimed to determine the impact of maternal FA supplementation on psychomotor skills and learning and memory functions in their adult offspring. Methods: Eighteen female Wistar rats were randomly divided into three groups. The animals were fed three different concentrations of FA from preconception to pregnancy and during lactation. The adult offspring were assessed for neurobehavioural changes and histological confirmation by hippocampal neuron quantification. Results: Neurobehavioural assessment revealed a significantly smaller number of alternations, a higher percentage bias, and a greater number of working and reference memory errors. The increased time spent in the dark compartment in the FA-supplementation group indicated deficit(s) in learning memory. Hippocampal neuron quantification revealed a higher mean number of viable neurons in the cornu ammonis (CA) region in the control group (CA1 region, 31.2 ± 3.2; CA3 region, 23.2 ± 3.2), with a distinct nucleus in both regions, and least in the FA-supplementation group (CA1 region, 24.2 ± 3.1; CA3 region, 15.2 ± 2.2). Conclusion: Results of this investigation support the possible negative effect of high levels of maternal FA supplementation during pregnancy and lactation. Such alterations potentially lead to neurobehavioural changes in the adult offspring of Wistar rats.

UR - http://www.scopus.com/inward/record.url?scp=85075896316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85075896316&partnerID=8YFLogxK

U2 - 10.1016/j.jtumed.2019.09.009

DO - 10.1016/j.jtumed.2019.09.009

M3 - Article

AN - SCOPUS:85075896316

JO - Journal of Taibah University Medical Sciences

JF - Journal of Taibah University Medical Sciences

SN - 1658-3612

ER -