Development and evaluation of fiber optic probe-based helium-neon low-level laser therapy system for tissue regeneration-an in vivo experimental study

Vijendra Prabhu, Satish B.S. Rao, Nageshwara B. Rao, Kiran B. Aithal, Pramod Kumar, Krishna K. Mahato

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26 Citations (Scopus)

Abstract

We report the design and development of an optical fiber probe-based Helium-Neon (He-Ne) low-level laser therapy system for tissue regeneration. Full thickness excision wounds on Swiss albino mice of diameter 15 mm were exposed to various laser doses of 1, 2, 3, 4, 6, 8 and 10 J cm-2 of the system with appropriate controls, and 2 J cm-2 showing optimum healing was selected. The treatment schedule for applying the selected laser dose was also standardized by irradiating the wounds at different postwounding times (0, 24 and 48 h). The tissue regeneration potential was evaluated by monitoring the progression of wound contraction and mean wound healing time along with the hydroxyproline and glucosamine estimation on wound ground tissues. The wounds exposed to 2 J cm-2 immediately after wounding showed considerable contraction on days 5, 9, 12, 14, 16 and 19 of postirradiation compared with the controls and other treatment schedules, showing significant (P < 0.001) decrease in the healing time. A significant increase in hydroxyproline and glucosamine levels was observed for the 2 J cm-2 irradiation group compared with the controls and other treatment groups. In conclusion, the wounds treated with 2 J cm-2 immediately after the wounding show better healing compared with the controls.

Original languageEnglish
Pages (from-to)1364-1372
Number of pages9
JournalPhotochemistry and Photobiology
Volume86
Issue number6
DOIs
Publication statusPublished - 11-2010

Fingerprint

Neon
Tissue regeneration
Helium
regeneration
neon
Fiber optics
Regeneration
fiber optics
therapy
healing
helium
Lasers
evaluation
probes
Glucosamine
Hydroxyproline
Wounds and Injuries
schedules
contraction
lasers

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

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title = "Development and evaluation of fiber optic probe-based helium-neon low-level laser therapy system for tissue regeneration-an in vivo experimental study",
abstract = "We report the design and development of an optical fiber probe-based Helium-Neon (He-Ne) low-level laser therapy system for tissue regeneration. Full thickness excision wounds on Swiss albino mice of diameter 15 mm were exposed to various laser doses of 1, 2, 3, 4, 6, 8 and 10 J cm-2 of the system with appropriate controls, and 2 J cm-2 showing optimum healing was selected. The treatment schedule for applying the selected laser dose was also standardized by irradiating the wounds at different postwounding times (0, 24 and 48 h). The tissue regeneration potential was evaluated by monitoring the progression of wound contraction and mean wound healing time along with the hydroxyproline and glucosamine estimation on wound ground tissues. The wounds exposed to 2 J cm-2 immediately after wounding showed considerable contraction on days 5, 9, 12, 14, 16 and 19 of postirradiation compared with the controls and other treatment schedules, showing significant (P < 0.001) decrease in the healing time. A significant increase in hydroxyproline and glucosamine levels was observed for the 2 J cm-2 irradiation group compared with the controls and other treatment groups. In conclusion, the wounds treated with 2 J cm-2 immediately after the wounding show better healing compared with the controls.",
author = "Vijendra Prabhu and Rao, {Satish B.S.} and Rao, {Nageshwara B.} and Aithal, {Kiran B.} and Pramod Kumar and Mahato, {Krishna K.}",
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AU - Rao, Satish B.S.

AU - Rao, Nageshwara B.

AU - Aithal, Kiran B.

AU - Kumar, Pramod

AU - Mahato, Krishna K.

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