Abstract
Ezetimibe belongs to a group of selective and very effective 2-azetidione cholesterol absorption inhibitors that act on the level of cholesterol entry into enterocytes. A new, rapid, specific reversed-phase HPLC method has been developed for assaying ezetimibe in pharmaceutical dosage forms. The assay involved an isocratic elution of ezetimibe in a Betasil C18 (250 mm x 4.6 mm i.d., 5μ) column using a mobile phase composition of 18 Acetonitrile: 10 mM Na2 HPO4 pH 7.0 (55:45 v/v). The flow rate was 1ml/min and the analyte monitored at 233nm. The assay method was found to be linear from 4-24μg/mL. All the validation parameters were within the acceptance range. The developed method was successfully applied to estimate the amount of ezetimibe in tablets.
| Original language | English |
|---|---|
| Pages (from-to) | 7-12 |
| Number of pages | 6 |
| Journal | Indian Journal of Pharmaceutical Education and Research |
| Volume | 47 |
| Issue number | 1 |
| Publication status | Published - 01-01-2013 |
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All Science Journal Classification (ASJC) codes
- Pharmacology, Toxicology and Pharmaceutics(all)
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Development and validation of a new reversed-phase HPLC method for the determination of ezetimibe in pharmaceutical dosage forms. / Panjwani, Ghanshyam; Shetty, Ragvendra; Shenoy, Gautham; Dhamecha, Dinesh.
In: Indian Journal of Pharmaceutical Education and Research, Vol. 47, No. 1, 01.01.2013, p. 7-12.Research output: Contribution to journal › Article
TY - JOUR
T1 - Development and validation of a new reversed-phase HPLC method for the determination of ezetimibe in pharmaceutical dosage forms
AU - Panjwani, Ghanshyam
AU - Shetty, Ragvendra
AU - Shenoy, Gautham
AU - Dhamecha, Dinesh
PY - 2013/1/1
Y1 - 2013/1/1
N2 - Ezetimibe belongs to a group of selective and very effective 2-azetidione cholesterol absorption inhibitors that act on the level of cholesterol entry into enterocytes. A new, rapid, specific reversed-phase HPLC method has been developed for assaying ezetimibe in pharmaceutical dosage forms. The assay involved an isocratic elution of ezetimibe in a Betasil C18 (250 mm x 4.6 mm i.d., 5μ) column using a mobile phase composition of 18 Acetonitrile: 10 mM Na2 HPO4 pH 7.0 (55:45 v/v). The flow rate was 1ml/min and the analyte monitored at 233nm. The assay method was found to be linear from 4-24μg/mL. All the validation parameters were within the acceptance range. The developed method was successfully applied to estimate the amount of ezetimibe in tablets.
AB - Ezetimibe belongs to a group of selective and very effective 2-azetidione cholesterol absorption inhibitors that act on the level of cholesterol entry into enterocytes. A new, rapid, specific reversed-phase HPLC method has been developed for assaying ezetimibe in pharmaceutical dosage forms. The assay involved an isocratic elution of ezetimibe in a Betasil C18 (250 mm x 4.6 mm i.d., 5μ) column using a mobile phase composition of 18 Acetonitrile: 10 mM Na2 HPO4 pH 7.0 (55:45 v/v). The flow rate was 1ml/min and the analyte monitored at 233nm. The assay method was found to be linear from 4-24μg/mL. All the validation parameters were within the acceptance range. The developed method was successfully applied to estimate the amount of ezetimibe in tablets.
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M3 - Article
VL - 47
SP - 7
EP - 12
JO - Indian Journal of Pharmaceutical Education and Research
JF - Indian Journal of Pharmaceutical Education and Research
SN - 0019-5464
IS - 1
ER -