Development and validation of a reversed-phase high-performance liquid chromatographic method for quantification of peptide dendrimers in human skin permeation experiments

S. Mutalik, A. K. Hewavitharana, P. N. Shaw, Y. G. Anissimov, M. S. Roberts, H. S. Parekh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The aim of the present work was to develop and validate a simple RP-HPLC method with UV detection to quantify peptide dendrimers in skin permeation experiments. Six dendrimers of varying positive charges (4+, 8+ and 16+) containing either histidine or arginine as terminal aminoacids were prepared by solid phase peptide synthesis. Mobile phase containing 0.02% (v/v) heptafluorobutyric acid in 90% acetonitrile-water was capable of separating all dendrimers from interfering peaks of receptor fluid. For the calibration of each dendrimer, a different dendrimer from the same class was selected as the internal standard. The results of preliminary human skin permeation studies showed that the developed analytical method can be successfully used for the quantification of cationic poly(aminoacid)-based dendrimers in skin permeation experiments.

Original languageEnglish
Pages (from-to)3556-3562
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number29
DOIs
Publication statusPublished - 01-11-2009

Fingerprint

Dendrimers
Permeation
Skin
Peptides
Liquids
Experiments
Solid-Phase Synthesis Techniques
Histidine
Calibration
Arginine
High Pressure Liquid Chromatography
Fluids
Water

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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abstract = "The aim of the present work was to develop and validate a simple RP-HPLC method with UV detection to quantify peptide dendrimers in skin permeation experiments. Six dendrimers of varying positive charges (4+, 8+ and 16+) containing either histidine or arginine as terminal aminoacids were prepared by solid phase peptide synthesis. Mobile phase containing 0.02{\%} (v/v) heptafluorobutyric acid in 90{\%} acetonitrile-water was capable of separating all dendrimers from interfering peaks of receptor fluid. For the calibration of each dendrimer, a different dendrimer from the same class was selected as the internal standard. The results of preliminary human skin permeation studies showed that the developed analytical method can be successfully used for the quantification of cationic poly(aminoacid)-based dendrimers in skin permeation experiments.",
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Development and validation of a reversed-phase high-performance liquid chromatographic method for quantification of peptide dendrimers in human skin permeation experiments. / Mutalik, S.; Hewavitharana, A. K.; Shaw, P. N.; Anissimov, Y. G.; Roberts, M. S.; Parekh, H. S.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 29, 01.11.2009, p. 3556-3562.

Research output: Contribution to journalArticle

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