Development and validation of an HPLC analytical method for the detection and quantification of nicardipine in lipid nanoparticles

Amanda V. Fernandes, Chinna R. Pydi, Girish Pai K, Ruchi Verma, Lalit Kumar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An HPLC method was developed and validated as per ICH guidelines for nicardipine analysis in lipid nanoparticles. Nicardipine was quantified using methanol and 40 mM ammonium formate (pH 3.5) with a ratio 65:35% v/v and BDS C18 column as a stationary phase. Detection of drug was carried out using UV detector at 240 nm by maintaining the flow rate at 1.0 mL/min. Retention time of nicardipine was found to be 4.67 min. Linearity was constructed between 0.50 to 32.00 µg/mL. Percent relative standard deviation (%RSD) from intraday and interday precisions were found to be less than 1% and 2%, respectively. Nicardipine recovery was found from 93.94 ± 0.51 to 100.16 ± 0.18%. LOD and LOQ values were found to be 25.50 ng/mL and 77.28 ng/mL, respectively. Robustness studies also confirmed that developed method is robust with %RSD < 2%. Study concludes that developed method can be used for analysis of nicardipine in lipid nanoparticles.

Original languageEnglish
Pages (from-to)1995-2000
Number of pages6
JournalLatin American Journal of Pharmacy
Volume36
Issue number10
Publication statusPublished - 2017

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery

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