An HPLC method was developed and validated as per ICH guidelines for nicardipine analysis in lipid nanoparticles. Nicardipine was quantified using methanol and 40 mM ammonium formate (pH 3.5) with a ratio 65:35% v/v and BDS C18 column as a stationary phase. Detection of drug was carried out using UV detector at 240 nm by maintaining the flow rate at 1.0 mL/min. Retention time of nicardipine was found to be 4.67 min. Linearity was constructed between 0.50 to 32.00 µg/mL. Percent relative standard deviation (%RSD) from intraday and interday precisions were found to be less than 1% and 2%, respectively. Nicardipine recovery was found from 93.94 ± 0.51 to 100.16 ± 0.18%. LOD and LOQ values were found to be 25.50 ng/mL and 77.28 ng/mL, respectively. Robustness studies also confirmed that developed method is robust with %RSD < 2%. Study concludes that developed method can be used for analysis of nicardipine in lipid nanoparticles.
|Number of pages||6|
|Journal||Latin American Journal of Pharmacy|
|Publication status||Published - 2017|
All Science Journal Classification (ASJC) codes
- Pharmaceutical Science
- Drug Discovery