Development and validation of RP-HPLC method for estimation of epigallocatechin -3-gallate (EGCG) in lipid based nanoformulations

Kiran S. Avadhani, Muthukumar Amirthalingam, Meka Sreenivasa Reddy, Nayanabhirama Udupa, Srinivas Mutalik

Research output: Contribution to journalArticle

Abstract

Epigallocatechin-3-gallate (EGCG) is a potent antioxidant and free radical scavenger present in green tea. It is extensively used as antioxidant, immunomodulator, anticancer, neuroprotective, cardioprotective, antiviral, antibacterial, photo protective and anti aging agent. The objective of the present work was to develop and validate an RP-HPLC method for determination of EGCG in lipid based nanoformulations. RP-HPLC analysis was performed by isocratic elution using a Phenomenex C18 column (4.6 × 250 mm, 5 μm) at 25 °C. A mixture of acetonitrile and 1% v/v acetic acid (25:75) of pH 2.8 was used as a mobile phase with a flow rate of 0.8 mL/min. The analysis was performed using a UV detector at 280 nm. The results showed that the peak area response was linear within the concentration range of 0.5-40 μg/mL (r =0.9998). The values of LOD (limit of detection) and LOQ (limit of quantification) were 0.125 μg/mL and 0.272 μg/mL respectively. The % RSD for peak area of intraday and interday precision was 0.08 and 0.2%, respectively. The results of accuracy for the different concentrations of EGCG (8, 10 and 12 μg/mL) were 100.29±0.19, 101.10±0.10 and 100.44±0.25 % respectively. The validated method was successfully applied for the estimation of EGCG content in lipid based nanoformulations.

Original languageEnglish
Pages (from-to)725-730
Number of pages6
JournalResearch Journal of Pharmacy and Technology
Volume9
Issue number6
DOIs
Publication statusPublished - 2016

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High Pressure Liquid Chromatography
Lipids
Antioxidants
Free Radical Scavengers
Immunologic Factors
Tea
Acetic Acid
Antiviral Agents
Limit of Detection
epigallocatechin gallate

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
  • Pharmacology (medical)

Cite this

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title = "Development and validation of RP-HPLC method for estimation of epigallocatechin -3-gallate (EGCG) in lipid based nanoformulations",
abstract = "Epigallocatechin-3-gallate (EGCG) is a potent antioxidant and free radical scavenger present in green tea. It is extensively used as antioxidant, immunomodulator, anticancer, neuroprotective, cardioprotective, antiviral, antibacterial, photo protective and anti aging agent. The objective of the present work was to develop and validate an RP-HPLC method for determination of EGCG in lipid based nanoformulations. RP-HPLC analysis was performed by isocratic elution using a Phenomenex C18 column (4.6 × 250 mm, 5 μm) at 25 °C. A mixture of acetonitrile and 1{\%} v/v acetic acid (25:75) of pH 2.8 was used as a mobile phase with a flow rate of 0.8 mL/min. The analysis was performed using a UV detector at 280 nm. The results showed that the peak area response was linear within the concentration range of 0.5-40 μg/mL (r =0.9998). The values of LOD (limit of detection) and LOQ (limit of quantification) were 0.125 μg/mL and 0.272 μg/mL respectively. The {\%} RSD for peak area of intraday and interday precision was 0.08 and 0.2{\%}, respectively. The results of accuracy for the different concentrations of EGCG (8, 10 and 12 μg/mL) were 100.29±0.19, 101.10±0.10 and 100.44±0.25 {\%} respectively. The validated method was successfully applied for the estimation of EGCG content in lipid based nanoformulations.",
author = "Avadhani, {Kiran S.} and Muthukumar Amirthalingam and Reddy, {Meka Sreenivasa} and Nayanabhirama Udupa and Srinivas Mutalik",
year = "2016",
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T1 - Development and validation of RP-HPLC method for estimation of epigallocatechin -3-gallate (EGCG) in lipid based nanoformulations

AU - Avadhani, Kiran S.

AU - Amirthalingam, Muthukumar

AU - Reddy, Meka Sreenivasa

AU - Udupa, Nayanabhirama

AU - Mutalik, Srinivas

PY - 2016

Y1 - 2016

N2 - Epigallocatechin-3-gallate (EGCG) is a potent antioxidant and free radical scavenger present in green tea. It is extensively used as antioxidant, immunomodulator, anticancer, neuroprotective, cardioprotective, antiviral, antibacterial, photo protective and anti aging agent. The objective of the present work was to develop and validate an RP-HPLC method for determination of EGCG in lipid based nanoformulations. RP-HPLC analysis was performed by isocratic elution using a Phenomenex C18 column (4.6 × 250 mm, 5 μm) at 25 °C. A mixture of acetonitrile and 1% v/v acetic acid (25:75) of pH 2.8 was used as a mobile phase with a flow rate of 0.8 mL/min. The analysis was performed using a UV detector at 280 nm. The results showed that the peak area response was linear within the concentration range of 0.5-40 μg/mL (r =0.9998). The values of LOD (limit of detection) and LOQ (limit of quantification) were 0.125 μg/mL and 0.272 μg/mL respectively. The % RSD for peak area of intraday and interday precision was 0.08 and 0.2%, respectively. The results of accuracy for the different concentrations of EGCG (8, 10 and 12 μg/mL) were 100.29±0.19, 101.10±0.10 and 100.44±0.25 % respectively. The validated method was successfully applied for the estimation of EGCG content in lipid based nanoformulations.

AB - Epigallocatechin-3-gallate (EGCG) is a potent antioxidant and free radical scavenger present in green tea. It is extensively used as antioxidant, immunomodulator, anticancer, neuroprotective, cardioprotective, antiviral, antibacterial, photo protective and anti aging agent. The objective of the present work was to develop and validate an RP-HPLC method for determination of EGCG in lipid based nanoformulations. RP-HPLC analysis was performed by isocratic elution using a Phenomenex C18 column (4.6 × 250 mm, 5 μm) at 25 °C. A mixture of acetonitrile and 1% v/v acetic acid (25:75) of pH 2.8 was used as a mobile phase with a flow rate of 0.8 mL/min. The analysis was performed using a UV detector at 280 nm. The results showed that the peak area response was linear within the concentration range of 0.5-40 μg/mL (r =0.9998). The values of LOD (limit of detection) and LOQ (limit of quantification) were 0.125 μg/mL and 0.272 μg/mL respectively. The % RSD for peak area of intraday and interday precision was 0.08 and 0.2%, respectively. The results of accuracy for the different concentrations of EGCG (8, 10 and 12 μg/mL) were 100.29±0.19, 101.10±0.10 and 100.44±0.25 % respectively. The validated method was successfully applied for the estimation of EGCG content in lipid based nanoformulations.

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