Development and validation of RP-HPLC method for the estimation of granisetron hydrochloride in bulk and tablets

S. Lakshmana Prabu, C. Dinesh Kumar, N. Sivagurunathan, S.G. Vasantharaju, A. Jacob

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A simple, precise and rapid RP-HPLC method was developed for the determination of Granisetron hydrochloride in pharmaceutical formulation. The method was carried out on a phenomenex RP-C18 column using a mixture of methanol and water (75:25). Detection was done at 301nm. The linearity range was 5 - 100.0mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The percentage recovery was found to be between the ranges of 98.81% to 99.30%.
Original languageEnglish
Pages (from-to)66-69
Number of pages4
JournalIndian Drugs
Volume47
Issue number1
Publication statusPublished - 2010

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Granisetron
Tablets
High Pressure Liquid Chromatography
Drug Compounding
Dosage Forms
Methanol
Water

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Lakshmana Prabu, S., Dinesh Kumar, C., Sivagurunathan, N., Vasantharaju, S. G., & Jacob, A. (2010). Development and validation of RP-HPLC method for the estimation of granisetron hydrochloride in bulk and tablets. Indian Drugs, 47(1), 66-69.
Lakshmana Prabu, S. ; Dinesh Kumar, C. ; Sivagurunathan, N. ; Vasantharaju, S.G. ; Jacob, A. / Development and validation of RP-HPLC method for the estimation of granisetron hydrochloride in bulk and tablets. In: Indian Drugs. 2010 ; Vol. 47, No. 1. pp. 66-69.
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abstract = "A simple, precise and rapid RP-HPLC method was developed for the determination of Granisetron hydrochloride in pharmaceutical formulation. The method was carried out on a phenomenex RP-C18 column using a mixture of methanol and water (75:25). Detection was done at 301nm. The linearity range was 5 - 100.0mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The percentage recovery was found to be between the ranges of 98.81{\%} to 99.30{\%}.",
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note = "Cited By :2 Export Date: 10 November 2017 CODEN: INDRB Correspondence Address: Dinesh Kumar, C.; Manipal College of Pharmaceutical Sciences, Manipal - 576 104, Karnataka, India; email: c_dinesh78@yahoo.co.in Chemicals/CAS: granisetron, 107007-99-8, 109889-09-0; methanol, 67-56-1; water, 7732-18-5 References: Jiang, Y., Lin, M., Fan, G., Chen, Y., Li, Z., Zhao, W., Wu, Y., Hu, J., Rapid determination of granisetron in human plasma by liquid chromatography coupled to tandem mass spectrometry and its application to bioequivalence study (2006) Journal of Pharmaceutical and Biomedical Analysis, 42 (4), pp. 464-473. , DOI 10.1016/j.jpba.2006.05.001, PII S0731708506003608; Boppana, V.K., Simultaneous determination of granisetron and its 7-hydroxy metabolite in human plasma by reverse phase high performance liquid chromatography utilizing fluorescence and electrochemical detection (1995) J Chromatogr. A, 692, pp. 195-202; Huang, C.T., Chen, C.F., Chen, K.C., Tsai, T.H., Simultaneous measurement of blood and brain microdialysates of granisetron in rat by high performance liquid chromatography with fluorescence detection (1998) J Chromatogr. B, 716, pp. 251-255; Wada, I., Satoh, M., Takeda, T., Nakabayashi, T., Honma, T., Saitoh, H., Takada, M., Hirano, K., A rapid assay of granisetron in biological fluids from cancer patients (1998) Biological and Pharmaceutical Bulletin, 21 (5), pp. 535-537; McElvain, J.S., Vandiver, V.J., Eichemeir, L.S., Validation of a reverse phase HPLC method for directly quantifying the enantiomers of MDL 74,156, the primary metabolite of dolasetron mesylate in human plasma (1997) J Pharm. Biomed. Anal., 15, pp. 513-521; Pinguet, F., Bressolle, F., Martel, P., Salabert, D., Astre, C., High-performance liquid chromatographic determination of granisetron in human plasma (1996) Journal of Chromatography B: Biomedical Applications, 675 (1), pp. 99-105. , DOI 10.1016/0378-4347(95)00335-5; Cupissol, D., Bressolle, F., Adenis, L., Carmichael, J., Bessell, E., Allen, A., Wargenau, M., Romain, D., Evaluation of the bioequivalence of tablet and capsule formulations of granisetron in patients undergoing cytotoxic chemotherapy for malignant disease (1993) Journal of Pharmaceutical Sciences, 82 (12), pp. 1281-1284. , DOI 10.1002/jps.2600821221; Capacio, B.R., Byers, C.E., Jackson, T.K., Matthews, R.L., An HPLC method for the determination of granisetron in guinea pig plasma (1993) Journal of Analytical Toxicology, 17 (3), pp. 151-155; Nirogi, R.V.S., Kandikere, V.N., Shukla, M., Mudigonda, K., Maurya, S., Boosi, R., Quantification of granisetron in human plasma by liquid chromatography coupled to electrospray tanden mass spectrometry (2006) Biomed. Chromatogr., 21, pp. 241-248",
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Lakshmana Prabu, S, Dinesh Kumar, C, Sivagurunathan, N, Vasantharaju, SG & Jacob, A 2010, 'Development and validation of RP-HPLC method for the estimation of granisetron hydrochloride in bulk and tablets', Indian Drugs, vol. 47, no. 1, pp. 66-69.

Development and validation of RP-HPLC method for the estimation of granisetron hydrochloride in bulk and tablets. / Lakshmana Prabu, S.; Dinesh Kumar, C.; Sivagurunathan, N.; Vasantharaju, S.G.; Jacob, A.

In: Indian Drugs, Vol. 47, No. 1, 2010, p. 66-69.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development and validation of RP-HPLC method for the estimation of granisetron hydrochloride in bulk and tablets

AU - Lakshmana Prabu, S.

AU - Dinesh Kumar, C.

AU - Sivagurunathan, N.

AU - Vasantharaju, S.G.

AU - Jacob, A.

N1 - Cited By :2 Export Date: 10 November 2017 CODEN: INDRB Correspondence Address: Dinesh Kumar, C.; Manipal College of Pharmaceutical Sciences, Manipal - 576 104, Karnataka, India; email: c_dinesh78@yahoo.co.in Chemicals/CAS: granisetron, 107007-99-8, 109889-09-0; methanol, 67-56-1; water, 7732-18-5 References: Jiang, Y., Lin, M., Fan, G., Chen, Y., Li, Z., Zhao, W., Wu, Y., Hu, J., Rapid determination of granisetron in human plasma by liquid chromatography coupled to tandem mass spectrometry and its application to bioequivalence study (2006) Journal of Pharmaceutical and Biomedical Analysis, 42 (4), pp. 464-473. , DOI 10.1016/j.jpba.2006.05.001, PII S0731708506003608; Boppana, V.K., Simultaneous determination of granisetron and its 7-hydroxy metabolite in human plasma by reverse phase high performance liquid chromatography utilizing fluorescence and electrochemical detection (1995) J Chromatogr. A, 692, pp. 195-202; Huang, C.T., Chen, C.F., Chen, K.C., Tsai, T.H., Simultaneous measurement of blood and brain microdialysates of granisetron in rat by high performance liquid chromatography with fluorescence detection (1998) J Chromatogr. B, 716, pp. 251-255; Wada, I., Satoh, M., Takeda, T., Nakabayashi, T., Honma, T., Saitoh, H., Takada, M., Hirano, K., A rapid assay of granisetron in biological fluids from cancer patients (1998) Biological and Pharmaceutical Bulletin, 21 (5), pp. 535-537; McElvain, J.S., Vandiver, V.J., Eichemeir, L.S., Validation of a reverse phase HPLC method for directly quantifying the enantiomers of MDL 74,156, the primary metabolite of dolasetron mesylate in human plasma (1997) J Pharm. Biomed. Anal., 15, pp. 513-521; Pinguet, F., Bressolle, F., Martel, P., Salabert, D., Astre, C., High-performance liquid chromatographic determination of granisetron in human plasma (1996) Journal of Chromatography B: Biomedical Applications, 675 (1), pp. 99-105. , DOI 10.1016/0378-4347(95)00335-5; Cupissol, D., Bressolle, F., Adenis, L., Carmichael, J., Bessell, E., Allen, A., Wargenau, M., Romain, D., Evaluation of the bioequivalence of tablet and capsule formulations of granisetron in patients undergoing cytotoxic chemotherapy for malignant disease (1993) Journal of Pharmaceutical Sciences, 82 (12), pp. 1281-1284. , DOI 10.1002/jps.2600821221; Capacio, B.R., Byers, C.E., Jackson, T.K., Matthews, R.L., An HPLC method for the determination of granisetron in guinea pig plasma (1993) Journal of Analytical Toxicology, 17 (3), pp. 151-155; Nirogi, R.V.S., Kandikere, V.N., Shukla, M., Mudigonda, K., Maurya, S., Boosi, R., Quantification of granisetron in human plasma by liquid chromatography coupled to electrospray tanden mass spectrometry (2006) Biomed. Chromatogr., 21, pp. 241-248

PY - 2010

Y1 - 2010

N2 - A simple, precise and rapid RP-HPLC method was developed for the determination of Granisetron hydrochloride in pharmaceutical formulation. The method was carried out on a phenomenex RP-C18 column using a mixture of methanol and water (75:25). Detection was done at 301nm. The linearity range was 5 - 100.0mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The percentage recovery was found to be between the ranges of 98.81% to 99.30%.

AB - A simple, precise and rapid RP-HPLC method was developed for the determination of Granisetron hydrochloride in pharmaceutical formulation. The method was carried out on a phenomenex RP-C18 column using a mixture of methanol and water (75:25). Detection was done at 301nm. The linearity range was 5 - 100.0mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The percentage recovery was found to be between the ranges of 98.81% to 99.30%.

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VL - 47

SP - 66

EP - 69

JO - Indian Drugs

JF - Indian Drugs

SN - 0019-462X

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ER -