Development of cotton transgenics with antisense AV2 gene for resistance against cotton leaf curl virus (CLCuD) via Agrobacterium tumefaciens

Sanjaya, V. V. Satyavathi, V. Prasad, N. Kirthi, S. P. Maiya, H. S. Savithri, G. Lakshmi Sita

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Cotton transgenics for resistance against cotton leaf curl disease using antisense movement protein gene (AV2) were developed in an Indian variety (F846) via Agrobacterium-mediated transformation using the protocol developed previously. A binary vector pPZP carrying the antisense AV2 (350 bp) gene along with the nptII gene was used. Transgenic nature of the putative transgenics was confirmed by molecular analysis. Shoots were induced on selection medium and subcultured on rooting medium containing IBA and 75 mg l-1 kanamycin. Transgenic plants were recovered in 12-16 weeks from the time of gene transfer to establishment in pots. Preliminary analysis of the field-established plantlets was conducted by PCR. T1 plants were obtained from T 0 seeds, the presence of the AV2 and nptII genes in the transgenic plants was verified by PCR and integration of T-DNA with AV2 into the plant genome of putative transgenics was further confirmed by Southern blot analysis. Several T1 lines were maintained in the greenhouse. Progeny analysis of these plants by PCR analysis showed a classical Mendelian pattern of inheritance.

Original languageEnglish
Pages (from-to)55-63
Number of pages9
JournalPlant Cell, Tissue and Organ Culture
Volume81
Issue number1
DOIs
Publication statusPublished - 01-04-2005
Externally publishedYes

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Agrobacterium radiobacter
cotton
genetically modified organisms
transgenic plants
genes
leaf curling
kanamycin
Agrobacterium
gene transfer
Southern blotting
plantlets
rooting
inheritance (genetics)
greenhouses
shoots
genome
Cotton leaf curl virus
DNA
seeds
proteins

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Sanjaya ; Satyavathi, V. V. ; Prasad, V. ; Kirthi, N. ; Maiya, S. P. ; Savithri, H. S. ; Lakshmi Sita, G. / Development of cotton transgenics with antisense AV2 gene for resistance against cotton leaf curl virus (CLCuD) via Agrobacterium tumefaciens. In: Plant Cell, Tissue and Organ Culture. 2005 ; Vol. 81, No. 1. pp. 55-63.
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Development of cotton transgenics with antisense AV2 gene for resistance against cotton leaf curl virus (CLCuD) via Agrobacterium tumefaciens. / Sanjaya; Satyavathi, V. V.; Prasad, V.; Kirthi, N.; Maiya, S. P.; Savithri, H. S.; Lakshmi Sita, G.

In: Plant Cell, Tissue and Organ Culture, Vol. 81, No. 1, 01.04.2005, p. 55-63.

Research output: Contribution to journalArticle

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