Development of HPLC assay method for the estimation of olanzapine in human plasma and its application to pharmacokinetics

B. Dasandi, P. Shah, C. Gandhi, K. M. Bhat, Shivprakash

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new method for the estimation of olanzapine in human plasma is developed and validated. It utilizes RP select B column and amperometric detector. The mobile phase consisted of 75mM sodium dihydrogen phosphate buffer of pH 7.0, acetonitrile and methanol in 55:22.5:22.5 %v/v ratios in an isocratic mode at 1.5ml-1 flow rate. The drug is extracted in to 3ml each of Tertiary Butyl Methyl Ether (TBME) twice from plasma, evaporated and the residue dissolved in mobile phase. The percentage extraction is found to be more than 79% and the linearity range from 0.5ng to 50.0ng ml-1. The method has been validated extensively and found to have good interday and intraday precision. This method has been applied to study pharmacokinetics in 12 healthy human volunteers.

Original languageEnglish
Pages (from-to)350-354
Number of pages5
JournalIndian Drugs
Volume40
Issue number6
Publication statusPublished - 01-06-2003

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olanzapine
Pharmacokinetics
High Pressure Liquid Chromatography
Methyl Ethers
Methanol
Healthy Volunteers
Buffers
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

Cite this

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abstract = "A new method for the estimation of olanzapine in human plasma is developed and validated. It utilizes RP select B column and amperometric detector. The mobile phase consisted of 75mM sodium dihydrogen phosphate buffer of pH 7.0, acetonitrile and methanol in 55:22.5:22.5 {\%}v/v ratios in an isocratic mode at 1.5ml-1 flow rate. The drug is extracted in to 3ml each of Tertiary Butyl Methyl Ether (TBME) twice from plasma, evaporated and the residue dissolved in mobile phase. The percentage extraction is found to be more than 79{\%} and the linearity range from 0.5ng to 50.0ng ml-1. The method has been validated extensively and found to have good interday and intraday precision. This method has been applied to study pharmacokinetics in 12 healthy human volunteers.",
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Development of HPLC assay method for the estimation of olanzapine in human plasma and its application to pharmacokinetics. / Dasandi, B.; Shah, P.; Gandhi, C.; Bhat, K. M.; Shivprakash.

In: Indian Drugs, Vol. 40, No. 6, 01.06.2003, p. 350-354.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Shah, P.

AU - Gandhi, C.

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AU - Shivprakash,

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