Development of new reversed-phase HPLC method for the determination of mangiferin in Mangifera indica Linn

P. Kumar, V. Srivastava, L. Kumar

Research output: Contribution to journalArticle

Abstract

Mangiferin is the active constituent obtained from the dried parts such as leaves and barks of the Mango tree (Mangifera indica L.) which belongs to the family Anacardiaceae. Analytical method is developed and validated for the determination of Mangiferin in Mangifera indica by reversed-phase HPLC method. The HPLC method is described for determination of Mangiferin from leaves and barks Extract. The separation of the Mangiferin was achieved on a Lichrospher ® 100; RP- 18e (5 μm) column using mobile phase Acetonitrile: Buffer (Potassium dihydrogen orthophosphate (1.36 gm) in 900 ml HPLC grade water, adjust the PH to 2.5-2.8 with orthophosphoric acid and detection was carried at 254 nm (Isocratic Elution). The Lichrospher ® 100; RP-18e (5 μm) shows most favorable chromatographic parameters for analysis of Mangiferin in Mangifera indica. The linear range for Mangiferin was found to be 15.0-1000 μg mL-1. The Method has been successfully validated for linearity, Specificity, Precision, Accuracy, range and with good recoveries. Hence the present RP-HPLC method is suitable for assay of the Mangiferin in raw materials, extracts in quality control laboratories.
Original languageEnglish
Pages (from-to)383-388
Number of pages6
JournalBiosciences Biotechnology Research Asia
Volume5
Issue number1
Publication statusPublished - 2008

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Mangifera
Mangifera indica
reversed-phase high performance liquid chromatography
High Pressure Liquid Chromatography
high performance liquid chromatography
bark
Anacardiaceae
orthophosphates
extracts
methodology
mangoes
quality control
analytical methods
raw materials
leaves
Plant Bark
buffers
potassium
mangiferin
acids

Cite this

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title = "Development of new reversed-phase HPLC method for the determination of mangiferin in Mangifera indica Linn",
abstract = "Mangiferin is the active constituent obtained from the dried parts such as leaves and barks of the Mango tree (Mangifera indica L.) which belongs to the family Anacardiaceae. Analytical method is developed and validated for the determination of Mangiferin in Mangifera indica by reversed-phase HPLC method. The HPLC method is described for determination of Mangiferin from leaves and barks Extract. The separation of the Mangiferin was achieved on a Lichrospher {\circledR} 100; RP- 18e (5 μm) column using mobile phase Acetonitrile: Buffer (Potassium dihydrogen orthophosphate (1.36 gm) in 900 ml HPLC grade water, adjust the PH to 2.5-2.8 with orthophosphoric acid and detection was carried at 254 nm (Isocratic Elution). The Lichrospher {\circledR} 100; RP-18e (5 μm) shows most favorable chromatographic parameters for analysis of Mangiferin in Mangifera indica. The linear range for Mangiferin was found to be 15.0-1000 μg mL-1. The Method has been successfully validated for linearity, Specificity, Precision, Accuracy, range and with good recoveries. Hence the present RP-HPLC method is suitable for assay of the Mangiferin in raw materials, extracts in quality control laboratories.",
author = "P. Kumar and V. Srivastava and L. Kumar",
note = "Export Date: 10 November 2017 Correspondence Address: Kumar, P.; Department of Pharmaceutical Analysis, PES College of Pharmacy, Bangalore, Karnataka, India Chemicals/CAS: mangiferin, 4773-96-0; phosphoric acid, 7664-38-2; potassium dihydrogen phosphate, 7778-77-0 References: The Ayurvedic Pharmacopoeia of India, by government of india Ministry of health and family welfare department of ISM and H, 1st Edition, Part -1, III, 9; Molecular formula and molecular weight of mangiferin. (Source: http://www.made-in-china.com/showroom/byro 2000/product-list/ Sap-Extract-1.html); Clasificaci{\'o}n of Mangifera indica. (Source: http://en.wikipedia.org/wiki/Mango); Parts used for the estimation of mangiferin in Mangifera indica. (Source: http://www.raintree.com/mango.htm); Ivan Ross, A., Medicinal plants of the world, p. 197. , Published by Humana Press Totowa, New Jersey; Rastogi, R.P., Mehrota, B.N., (1991) Compendium of Indian Medicinal Plants, pp. 265-266. , Central Drug Research Institute Lucknow; India; Rastogi, R.P., Mehrota, B.N., (1993) Compendium of indian Medicinal Plants, pp. 407-409. , Central Drug Research Institute Lucknow; India; Rastogi, R.P., Mehrota, B.N., (1995) Compendium of indian Medicinal Plants, p. 456. , Central Drug Research Institute Lucknow; India; Rastogi, R.P., Mehrota, B.N., (1998) Compendium of indian Medicinal Plants, p. 524. , Central Drug Research Institute Lucknow; India; Trease and Evans, A Textbook of Pharmacognosy, 14th Edition, 249UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-51049096851&partnerID=40&md5=49dc2cf66aebae45d0069e212d68d8fa",
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journal = "Biosciences Biotechnology Research Asia",
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Development of new reversed-phase HPLC method for the determination of mangiferin in Mangifera indica Linn. / Kumar, P.; Srivastava, V.; Kumar, L.

In: Biosciences Biotechnology Research Asia, Vol. 5, No. 1, 2008, p. 383-388.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Development of new reversed-phase HPLC method for the determination of mangiferin in Mangifera indica Linn

AU - Kumar, P.

AU - Srivastava, V.

AU - Kumar, L.

N1 - Export Date: 10 November 2017 Correspondence Address: Kumar, P.; Department of Pharmaceutical Analysis, PES College of Pharmacy, Bangalore, Karnataka, India Chemicals/CAS: mangiferin, 4773-96-0; phosphoric acid, 7664-38-2; potassium dihydrogen phosphate, 7778-77-0 References: The Ayurvedic Pharmacopoeia of India, by government of india Ministry of health and family welfare department of ISM and H, 1st Edition, Part -1, III, 9; Molecular formula and molecular weight of mangiferin. (Source: http://www.made-in-china.com/showroom/byro 2000/product-list/ Sap-Extract-1.html); Clasificación of Mangifera indica. (Source: http://en.wikipedia.org/wiki/Mango); Parts used for the estimation of mangiferin in Mangifera indica. (Source: http://www.raintree.com/mango.htm); Ivan Ross, A., Medicinal plants of the world, p. 197. , Published by Humana Press Totowa, New Jersey; Rastogi, R.P., Mehrota, B.N., (1991) Compendium of Indian Medicinal Plants, pp. 265-266. , Central Drug Research Institute Lucknow; India; Rastogi, R.P., Mehrota, B.N., (1993) Compendium of indian Medicinal Plants, pp. 407-409. , Central Drug Research Institute Lucknow; India; Rastogi, R.P., Mehrota, B.N., (1995) Compendium of indian Medicinal Plants, p. 456. , Central Drug Research Institute Lucknow; India; Rastogi, R.P., Mehrota, B.N., (1998) Compendium of indian Medicinal Plants, p. 524. , Central Drug Research Institute Lucknow; India; Trease and Evans, A Textbook of Pharmacognosy, 14th Edition, 249UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-51049096851&partnerID=40&md5=49dc2cf66aebae45d0069e212d68d8fa

PY - 2008

Y1 - 2008

N2 - Mangiferin is the active constituent obtained from the dried parts such as leaves and barks of the Mango tree (Mangifera indica L.) which belongs to the family Anacardiaceae. Analytical method is developed and validated for the determination of Mangiferin in Mangifera indica by reversed-phase HPLC method. The HPLC method is described for determination of Mangiferin from leaves and barks Extract. The separation of the Mangiferin was achieved on a Lichrospher ® 100; RP- 18e (5 μm) column using mobile phase Acetonitrile: Buffer (Potassium dihydrogen orthophosphate (1.36 gm) in 900 ml HPLC grade water, adjust the PH to 2.5-2.8 with orthophosphoric acid and detection was carried at 254 nm (Isocratic Elution). The Lichrospher ® 100; RP-18e (5 μm) shows most favorable chromatographic parameters for analysis of Mangiferin in Mangifera indica. The linear range for Mangiferin was found to be 15.0-1000 μg mL-1. The Method has been successfully validated for linearity, Specificity, Precision, Accuracy, range and with good recoveries. Hence the present RP-HPLC method is suitable for assay of the Mangiferin in raw materials, extracts in quality control laboratories.

AB - Mangiferin is the active constituent obtained from the dried parts such as leaves and barks of the Mango tree (Mangifera indica L.) which belongs to the family Anacardiaceae. Analytical method is developed and validated for the determination of Mangiferin in Mangifera indica by reversed-phase HPLC method. The HPLC method is described for determination of Mangiferin from leaves and barks Extract. The separation of the Mangiferin was achieved on a Lichrospher ® 100; RP- 18e (5 μm) column using mobile phase Acetonitrile: Buffer (Potassium dihydrogen orthophosphate (1.36 gm) in 900 ml HPLC grade water, adjust the PH to 2.5-2.8 with orthophosphoric acid and detection was carried at 254 nm (Isocratic Elution). The Lichrospher ® 100; RP-18e (5 μm) shows most favorable chromatographic parameters for analysis of Mangiferin in Mangifera indica. The linear range for Mangiferin was found to be 15.0-1000 μg mL-1. The Method has been successfully validated for linearity, Specificity, Precision, Accuracy, range and with good recoveries. Hence the present RP-HPLC method is suitable for assay of the Mangiferin in raw materials, extracts in quality control laboratories.

M3 - Article

VL - 5

SP - 383

EP - 388

JO - Biosciences Biotechnology Research Asia

JF - Biosciences Biotechnology Research Asia

SN - 0973-1245

IS - 1

ER -