Diagnostic efficacy of Ziehl-Neelsen method against fluorescent microscopy in detection of acid fast bacilli

Soham Gupta, Vishnu Prasad Shenoy, Indira Bairy, S. Muralidharan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective: To investigate the application of Ziehl-Neelsen (Z-N) and fluorescent microscopy in detection of acid fast bacilli (AFB). Methods: Duplicate smears were prepared from 260 sputum samples and stained with Z-N and fluorescent staining (FS) methods. The efficiency of both methods in primary diagnosis of tuberculosis were evaluated. Results: The smears were positive for AFB in 15 (5.77%) samples by Z-N staining method and in 16 (6.15%) samples by FS method. The sensitivity and specificity of Z-N staining method against FS method were 93.75% and 100% respectively. Conclusions: Though lesser cost-effective than Z-N, FS method is a more sensitive and better case finding tool in detection of AFB.

Original languageEnglish
Pages (from-to)328-329
Number of pages2
JournalAsian Pacific Journal of Tropical Medicine
Volume3
Issue number4
DOIs
Publication statusPublished - 01-04-2010

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Bacillus
Microscopy
Acids
Staining and Labeling
Sputum
Tuberculosis
Costs and Cost Analysis
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Diagnostic efficacy of Ziehl-Neelsen method against fluorescent microscopy in detection of acid fast bacilli",
abstract = "Objective: To investigate the application of Ziehl-Neelsen (Z-N) and fluorescent microscopy in detection of acid fast bacilli (AFB). Methods: Duplicate smears were prepared from 260 sputum samples and stained with Z-N and fluorescent staining (FS) methods. The efficiency of both methods in primary diagnosis of tuberculosis were evaluated. Results: The smears were positive for AFB in 15 (5.77{\%}) samples by Z-N staining method and in 16 (6.15{\%}) samples by FS method. The sensitivity and specificity of Z-N staining method against FS method were 93.75{\%} and 100{\%} respectively. Conclusions: Though lesser cost-effective than Z-N, FS method is a more sensitive and better case finding tool in detection of AFB.",
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Diagnostic efficacy of Ziehl-Neelsen method against fluorescent microscopy in detection of acid fast bacilli. / Gupta, Soham; Shenoy, Vishnu Prasad; Bairy, Indira; Muralidharan, S.

In: Asian Pacific Journal of Tropical Medicine, Vol. 3, No. 4, 01.04.2010, p. 328-329.

Research output: Contribution to journalArticle

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AU - Shenoy, Vishnu Prasad

AU - Bairy, Indira

AU - Muralidharan, S.

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