Diagnostic value of latex agglutination in cryptococcal meningitis

Rm Saldanha Dominic, Hv Prashanth, Shalini Shenoy, Shrikala Baliga

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Cryptococcus neoformans is the most common fungal pathogen to infect the central nervous system, and an effective diagnostic method is therefore necessary for the early diagnosis of cryptococcal meningitis.

AIM: The efficacy of India ink preparation, cerebrospinal fluid (CSF) culture and CSF cryptococcal antigen detection by the latex agglutination test for diagnosis of cryptococcal meningitis are compared to determine the most efficient test.

MATERIALS AND METHODS: Two hundred CSF samples from human immunodeficiency virus - positive patients suspected to be suffering from meningitis were screened for Cryptococcus neoformans.

RESULTS: Latex agglutination for cryptococcal antigen detection was found to be more sensitive compared to India ink staining and CSF culture.

CONCLUSIONS: Antigen detection by latex agglutination proved to be both sensitive and specific method for the diagnosis of cryptococcal meningitis. Rapid, early diagnosis of infection by detection of cryptococcal antigen by latex agglutination may alter the prognosis for these patients.

Original languageEnglish
Pages (from-to)67-8
Number of pages2
JournalJournal of laboratory physicians
Volume1
Issue number2
DOIs
Publication statusPublished - 07-2009

Fingerprint

Cryptococcal Meningitis
Agglutination
Latex
Cerebrospinal Fluid
Antigens
Cryptococcus neoformans
Early Diagnosis
Latex Fixation Tests
Meningitis
Central Nervous System
HIV
Staining and Labeling
Infection
chinese ink

Cite this

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title = "Diagnostic value of latex agglutination in cryptococcal meningitis",
abstract = "BACKGROUND: Cryptococcus neoformans is the most common fungal pathogen to infect the central nervous system, and an effective diagnostic method is therefore necessary for the early diagnosis of cryptococcal meningitis.AIM: The efficacy of India ink preparation, cerebrospinal fluid (CSF) culture and CSF cryptococcal antigen detection by the latex agglutination test for diagnosis of cryptococcal meningitis are compared to determine the most efficient test.MATERIALS AND METHODS: Two hundred CSF samples from human immunodeficiency virus - positive patients suspected to be suffering from meningitis were screened for Cryptococcus neoformans.RESULTS: Latex agglutination for cryptococcal antigen detection was found to be more sensitive compared to India ink staining and CSF culture.CONCLUSIONS: Antigen detection by latex agglutination proved to be both sensitive and specific method for the diagnosis of cryptococcal meningitis. Rapid, early diagnosis of infection by detection of cryptococcal antigen by latex agglutination may alter the prognosis for these patients.",
author = "Dominic, {Rm Saldanha} and Hv Prashanth and Shalini Shenoy and Shrikala Baliga",
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Diagnostic value of latex agglutination in cryptococcal meningitis. / Dominic, Rm Saldanha; Prashanth, Hv; Shenoy, Shalini; Baliga, Shrikala.

In: Journal of laboratory physicians, Vol. 1, No. 2, 07.2009, p. 67-8.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Diagnostic value of latex agglutination in cryptococcal meningitis

AU - Dominic, Rm Saldanha

AU - Prashanth, Hv

AU - Shenoy, Shalini

AU - Baliga, Shrikala

PY - 2009/7

Y1 - 2009/7

N2 - BACKGROUND: Cryptococcus neoformans is the most common fungal pathogen to infect the central nervous system, and an effective diagnostic method is therefore necessary for the early diagnosis of cryptococcal meningitis.AIM: The efficacy of India ink preparation, cerebrospinal fluid (CSF) culture and CSF cryptococcal antigen detection by the latex agglutination test for diagnosis of cryptococcal meningitis are compared to determine the most efficient test.MATERIALS AND METHODS: Two hundred CSF samples from human immunodeficiency virus - positive patients suspected to be suffering from meningitis were screened for Cryptococcus neoformans.RESULTS: Latex agglutination for cryptococcal antigen detection was found to be more sensitive compared to India ink staining and CSF culture.CONCLUSIONS: Antigen detection by latex agglutination proved to be both sensitive and specific method for the diagnosis of cryptococcal meningitis. Rapid, early diagnosis of infection by detection of cryptococcal antigen by latex agglutination may alter the prognosis for these patients.

AB - BACKGROUND: Cryptococcus neoformans is the most common fungal pathogen to infect the central nervous system, and an effective diagnostic method is therefore necessary for the early diagnosis of cryptococcal meningitis.AIM: The efficacy of India ink preparation, cerebrospinal fluid (CSF) culture and CSF cryptococcal antigen detection by the latex agglutination test for diagnosis of cryptococcal meningitis are compared to determine the most efficient test.MATERIALS AND METHODS: Two hundred CSF samples from human immunodeficiency virus - positive patients suspected to be suffering from meningitis were screened for Cryptococcus neoformans.RESULTS: Latex agglutination for cryptococcal antigen detection was found to be more sensitive compared to India ink staining and CSF culture.CONCLUSIONS: Antigen detection by latex agglutination proved to be both sensitive and specific method for the diagnosis of cryptococcal meningitis. Rapid, early diagnosis of infection by detection of cryptococcal antigen by latex agglutination may alter the prognosis for these patients.

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