Different phases of breast cancer cells

Raman study of immortalized, transformed, and invasive cells

Deepika Chaturvedi, Sai A. Balaji, Vinay Kumar Bn, Freek Ariese, Siva Umapathy, Annapoorni Rangarajan

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Breast cancer is the most prevalent cause of cancer-associated death in women the world over, but if detected early it can be treated successfully. Therefore, it is important to diagnose this disease at an early stage and to understand the biochemical changes associated with cellular transformation and cancer progression. Deregulated lipid metabolism has been shown to contribute to cell transformation as well as cancer progression. In this study, we monitored the biomolecular changes associated with the transformation of a normal cell into an invasive cell associated with breast cancer using Raman microspectroscopy. We have utilized primary normal breast cells, and immortalized, transformed, non-invasive, and invasive breast cancer cells. The Raman spectra were acquired from all these cell lines under physiological conditions. The higher wavenumber (2800-3000 cm-1) and lower wavenumber (700-1800 cm-1) range of the Raman spectrum were analyzed and we observed increased lipid levels for invasive cells. The Raman spectral data were analyzed by principal component-linear discriminant analysis (PC-LDA), which resulted in the formation of distinct clusters for different cell types with a high degree of sensitivity. The subsequent testing of the PC-LDA analysis via the leave-one-out cross validation approach (LOOCV) yielded relatively high identification sensitivity. Additionally, the Raman spectroscopic results were confirmed through fluorescence staining tests with BODIPY and Nile Red biochemical assays. Furthermore, Raman maps from the above mentioned cells under fixed conditions were also acquired to visualize the distribution of biomolecules throughout the cell. The present study shows the suitability of Raman spectroscopy as a non-invasive, label-free, microspectroscopic technique, having the potential of probing changes in the biomolecular composition of living cells as well as fixed cells.

Original languageEnglish
Article number57
JournalBiosensors
Volume6
Issue number4
DOIs
Publication statusPublished - 2016
Externally publishedYes

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Cells
Breast Neoplasms
Discriminant analysis
Raman scattering
Biomolecules
Raman spectroscopy
Labels
Assays
Discriminant Analysis
Fluorescence
Lipids
Testing
Chemical analysis
Neoplasms
Raman Spectrum Analysis
Lipid Metabolism
Breast
Staining and Labeling
Cell Line

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Chaturvedi, D., Balaji, S. A., Bn, V. K., Ariese, F., Umapathy, S., & Rangarajan, A. (2016). Different phases of breast cancer cells: Raman study of immortalized, transformed, and invasive cells. Biosensors, 6(4), [57]. https://doi.org/10.3390/bios6040057
Chaturvedi, Deepika ; Balaji, Sai A. ; Bn, Vinay Kumar ; Ariese, Freek ; Umapathy, Siva ; Rangarajan, Annapoorni. / Different phases of breast cancer cells : Raman study of immortalized, transformed, and invasive cells. In: Biosensors. 2016 ; Vol. 6, No. 4.
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Chaturvedi, D, Balaji, SA, Bn, VK, Ariese, F, Umapathy, S & Rangarajan, A 2016, 'Different phases of breast cancer cells: Raman study of immortalized, transformed, and invasive cells', Biosensors, vol. 6, no. 4, 57. https://doi.org/10.3390/bios6040057

Different phases of breast cancer cells : Raman study of immortalized, transformed, and invasive cells. / Chaturvedi, Deepika; Balaji, Sai A.; Bn, Vinay Kumar; Ariese, Freek; Umapathy, Siva; Rangarajan, Annapoorni.

In: Biosensors, Vol. 6, No. 4, 57, 2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Different phases of breast cancer cells

T2 - Raman study of immortalized, transformed, and invasive cells

AU - Chaturvedi, Deepika

AU - Balaji, Sai A.

AU - Bn, Vinay Kumar

AU - Ariese, Freek

AU - Umapathy, Siva

AU - Rangarajan, Annapoorni

PY - 2016

Y1 - 2016

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