Differential response of primary and metastatic melanomas to neutrophils attracted by IL-8

Helmut Schaider, Masahiro Oka, Thomas Bogenrieder, Mark Nesbit, Kapaettu Satyamoorthy, Carola Berking, Kouji Matsushima, Meenhard Herlyn

    Research output: Contribution to journalArticle

    109 Citations (Scopus)

    Abstract

    IL-8 is a strong chemoattractant for neutrophils, and it is constitutively produced by many tumors, including human melanomas. To determine the biologic importance of IL-8 for melanoma cells from primary and metastatic lesions, we transduced selected cell lines constitutively producing low levels of IL-8 with IL-8 cDNA using a replication-deficient adenoviral vector. Nontumorigenic SBcl2 primary melanoma cells formed tumors when transduced with increasing plaque-forming units of IL-8 per cell. However, at high IL-8 transduction levels (100 ng/ml/105 cells in 48 hr), tumor growth was impaired due to massive neutrophil infiltration. A similar biphasic response was observed in WMII5 primary melanomas, which are tumorigenic but not metastatic. Depletion of neutrophils with an antibody that blocks the accumulation of granulocytes at the site of inflammation enabled transduced primary melanomas secreting high levels of IL-8 to survive and grow. In contrast, highly tumorigenic and metastatic 45ILu cells showed marked increases in tumor growth and number of metastatic foci in the lungs depending on the expression levels of IL-8. Cytotoxicity assays with isolated neutrophils confirmed the preferential killing of primary over metastatic melanoma cells. SBcl2 cells stimulated by IL-8 to form tumors in immunodeficient mice were induced to produce VEGF, suggesting that the angiogenic response is enhanced due to increased growth factor production. Our results demonstrate that nontumorigenic primary melanomas depend on IL-8 stimulation in vivo for growth and that tumor growth depends on the level of neutrophil infiltration. Metastatic melanomas proliferate in vivo independently of infiltrating neutrophils.

    Original languageEnglish
    Pages (from-to)335-343
    Number of pages9
    JournalInternational Journal of Cancer
    Volume103
    Issue number3
    DOIs
    Publication statusPublished - 20-01-2003

    Fingerprint

    Interleukin-8
    Melanoma
    Neutrophils
    Neoplasms
    Neutrophil Infiltration
    Growth
    Chemotactic Factors
    Granulocytes
    Vascular Endothelial Growth Factor A
    Intercellular Signaling Peptides and Proteins
    Complementary DNA
    Inflammation
    Cell Line
    Lung
    Antibodies

    All Science Journal Classification (ASJC) codes

    • Cancer Research
    • Oncology

    Cite this

    Schaider, Helmut ; Oka, Masahiro ; Bogenrieder, Thomas ; Nesbit, Mark ; Satyamoorthy, Kapaettu ; Berking, Carola ; Matsushima, Kouji ; Herlyn, Meenhard. / Differential response of primary and metastatic melanomas to neutrophils attracted by IL-8. In: International Journal of Cancer. 2003 ; Vol. 103, No. 3. pp. 335-343.
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    abstract = "IL-8 is a strong chemoattractant for neutrophils, and it is constitutively produced by many tumors, including human melanomas. To determine the biologic importance of IL-8 for melanoma cells from primary and metastatic lesions, we transduced selected cell lines constitutively producing low levels of IL-8 with IL-8 cDNA using a replication-deficient adenoviral vector. Nontumorigenic SBcl2 primary melanoma cells formed tumors when transduced with increasing plaque-forming units of IL-8 per cell. However, at high IL-8 transduction levels (100 ng/ml/105 cells in 48 hr), tumor growth was impaired due to massive neutrophil infiltration. A similar biphasic response was observed in WMII5 primary melanomas, which are tumorigenic but not metastatic. Depletion of neutrophils with an antibody that blocks the accumulation of granulocytes at the site of inflammation enabled transduced primary melanomas secreting high levels of IL-8 to survive and grow. In contrast, highly tumorigenic and metastatic 45ILu cells showed marked increases in tumor growth and number of metastatic foci in the lungs depending on the expression levels of IL-8. Cytotoxicity assays with isolated neutrophils confirmed the preferential killing of primary over metastatic melanoma cells. SBcl2 cells stimulated by IL-8 to form tumors in immunodeficient mice were induced to produce VEGF, suggesting that the angiogenic response is enhanced due to increased growth factor production. Our results demonstrate that nontumorigenic primary melanomas depend on IL-8 stimulation in vivo for growth and that tumor growth depends on the level of neutrophil infiltration. Metastatic melanomas proliferate in vivo independently of infiltrating neutrophils.",
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    Schaider, H, Oka, M, Bogenrieder, T, Nesbit, M, Satyamoorthy, K, Berking, C, Matsushima, K & Herlyn, M 2003, 'Differential response of primary and metastatic melanomas to neutrophils attracted by IL-8', International Journal of Cancer, vol. 103, no. 3, pp. 335-343. https://doi.org/10.1002/ijc.10775

    Differential response of primary and metastatic melanomas to neutrophils attracted by IL-8. / Schaider, Helmut; Oka, Masahiro; Bogenrieder, Thomas; Nesbit, Mark; Satyamoorthy, Kapaettu; Berking, Carola; Matsushima, Kouji; Herlyn, Meenhard.

    In: International Journal of Cancer, Vol. 103, No. 3, 20.01.2003, p. 335-343.

    Research output: Contribution to journalArticle

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    AU - Oka, Masahiro

    AU - Bogenrieder, Thomas

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    AU - Herlyn, Meenhard

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