Differentiation of isolated and characterized human dental pulp stem cells and stem cells from human exfoliated deciduous teeth

An in vitro study

Vinay Rao Vishwanath, Roopa R. Nadig, Ramanand Nadig, Jyothi S. Prasanna, J. Karthik, Veena S. Pai

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aims and objectives: Isolation, characterization and differentiation of dental pulp stem cells (DPSCs) and stem cells from exfoliated human deciduous teeth (SHED). Methods: The pulp tissue was digested in collagenase and cultured in DMEM Dulbecco′s Modified Eagle′s Media). The stem cells were identified and isolated. Surface characterization of cells was done with flow cytometer using surface markers. An immuno cytochemistry analysis was done. Differentiation potential was analyzed using various differentiation markers. Results: Flow cytometry analyses for various CD markers showed similar results for both DPSCs and SHED. The cells showed positive expression for pluripotent, ectodermal and mesodermal markers. Cells differentiated into osteoblasts and adipocytes. Conclusion: The study demonstrated that stem cells existed in deciduous and permanent pulp tissue. The stem cells present in pulp tissue can be isolated, cultivated and expanded in vitro. Both DPSCs and SHED show almost a similar expression pattern profile for variety of antigens tested.

Original languageEnglish
Pages (from-to)423-428
Number of pages6
JournalJournal of Conservative Dentistry
Volume16
Issue number5
DOIs
Publication statusPublished - 09-2013

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Dental Pulp
Deciduous Tooth
Stem Cells
Histocytochemistry
Eagles
Differentiation Antigens
Collagenases
In Vitro Techniques
Osteoblasts
Adipocytes
Flow Cytometry
Antigens

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

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abstract = "Aims and objectives: Isolation, characterization and differentiation of dental pulp stem cells (DPSCs) and stem cells from exfoliated human deciduous teeth (SHED). Methods: The pulp tissue was digested in collagenase and cultured in DMEM Dulbecco′s Modified Eagle′s Media). The stem cells were identified and isolated. Surface characterization of cells was done with flow cytometer using surface markers. An immuno cytochemistry analysis was done. Differentiation potential was analyzed using various differentiation markers. Results: Flow cytometry analyses for various CD markers showed similar results for both DPSCs and SHED. The cells showed positive expression for pluripotent, ectodermal and mesodermal markers. Cells differentiated into osteoblasts and adipocytes. Conclusion: The study demonstrated that stem cells existed in deciduous and permanent pulp tissue. The stem cells present in pulp tissue can be isolated, cultivated and expanded in vitro. Both DPSCs and SHED show almost a similar expression pattern profile for variety of antigens tested.",
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Differentiation of isolated and characterized human dental pulp stem cells and stem cells from human exfoliated deciduous teeth : An in vitro study. / Vishwanath, Vinay Rao; Nadig, Roopa R.; Nadig, Ramanand; Prasanna, Jyothi S.; Karthik, J.; Pai, Veena S.

In: Journal of Conservative Dentistry, Vol. 16, No. 5, 09.2013, p. 423-428.

Research output: Contribution to journalArticle

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AU - Vishwanath, Vinay Rao

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AU - Pai, Veena S.

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