Discrimination of normal and malignant stomach mucosal tissues by Raman spectroscopy

A pilot study

K. Kalyan Kumar, A. Anand, M. V.P. Chowdary, Keerthi, Jacob Kurien, C. Murali Krishna, Stanley Mathew

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Stomach cancers are the fourth most common cancers and second most common cause of death from cancers worldwide. Two thirds of cases are reported from the developing world. The identified risk factors are consumption of traditionally salted meat or pickles, tobacco smoking and Helicobacter pylori infection. High intake of fruits and vegetables has shown to decrease the risk. Endoscopy with histopathology is the diagnostic investigation. Radical gastrectomy is the treatment of choice. Prognosis depends on the stage at the time of diagnosis. Conventional Raman spectroscopic studies of normal and malignant stomach tissues are carried out. The aim of the study is to explore the feasibility of discriminating these tissues. The mean Raman spectra of normal and malignant tissues exhibit significant differences in amide I, ΔCH2 and amide III region. The major spectral features of normal tissue with respect to malignant tissue are: weak amide I, slightly red shifted ΔCH2, an intense band at 1303 cm-1 and a hump at 1276 cm-1. Multivariate statistical tool, principal components analysis (PCA) is employed for developing discrimination methods. Scores of factors, Mahalanobis distance and spectral residuals are used as discriminating parameters. This approach is tested both retrospectively and prospectively. The analysis is further fine-tuned by employing 'Limit test' approach wherein reasonable discrimination between two groups, normal and malignant is observed for Mahalanobis distance of '3'. The results obtained in this pilot study indicate the feasibility of discriminating normal and malignant stomach mucosal tissues by Raman spectroscopy.

Original languageEnglish
Pages (from-to)382-387
Number of pages6
JournalVibrational Spectroscopy
Volume44
Issue number2
DOIs
Publication statusPublished - 17-07-2007

Fingerprint

Raman spectroscopy
Tissue
Amides
Endoscopy
Tobacco
Meats
Vegetables
Fruits
Principal component analysis
Raman scattering
Mucous Membrane

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Cite this

Kalyan Kumar, K. ; Anand, A. ; Chowdary, M. V.P. ; Keerthi ; Kurien, Jacob ; Murali Krishna, C. ; Mathew, Stanley. / Discrimination of normal and malignant stomach mucosal tissues by Raman spectroscopy : A pilot study. In: Vibrational Spectroscopy. 2007 ; Vol. 44, No. 2. pp. 382-387.
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Kalyan Kumar, K, Anand, A, Chowdary, MVP, Keerthi, Kurien, J, Murali Krishna, C & Mathew, S 2007, 'Discrimination of normal and malignant stomach mucosal tissues by Raman spectroscopy: A pilot study', Vibrational Spectroscopy, vol. 44, no. 2, pp. 382-387. https://doi.org/10.1016/j.vibspec.2007.03.007

Discrimination of normal and malignant stomach mucosal tissues by Raman spectroscopy : A pilot study. / Kalyan Kumar, K.; Anand, A.; Chowdary, M. V.P.; Keerthi; Kurien, Jacob; Murali Krishna, C.; Mathew, Stanley.

In: Vibrational Spectroscopy, Vol. 44, No. 2, 17.07.2007, p. 382-387.

Research output: Contribution to journalArticle

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Kalyan Kumar K, Anand A, Chowdary MVP, Keerthi, Kurien J, Murali Krishna C et al. Discrimination of normal and malignant stomach mucosal tissues by Raman spectroscopy: A pilot study. Vibrational Spectroscopy. 2007 Jul 17;44(2):382-387. https://doi.org/10.1016/j.vibspec.2007.03.007