DNA double-strand break analysis by γ-H2AX foci

A useful method for determining the overreactors to radiation-induced acute reactions among head-and-neck cancer patients

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Abstract

Purpose: Interindividual variability in normal tissue toxicity during radiation therapy is a limiting factor for successful treatment. Predicting the risk of developing acute reactions before initiation of radiation therapy may have the benefit of opting for altered radiation therapy regimens to achieve minimal adverse effects with improved tumor cure. Methods and Materials: DNA double-strand break (DSB) induction and its repair kinetics in lymphocytes of head-and-neck cancer patients undergoing chemoradiation therapy was analyzed by counting γ-H2AX foci, neutral comet assay, and a modified version of neutral filter elution assay. Acute normal tissue reactions were assessed by Radiation Therapy Oncology Group criteria. Results: The correlation between residual DSBs and the severity of acute reactions demonstrated that residual γ-H2AX foci in head-and-neck cancer patients increased with the severity of oral mucositis and skin reaction. Conclusions: Our results suggest that γ-H2AX analysis may have predictive implications for identifying the overreactors to mucositis and skin reactions among head-and-neck cancer patients prior to initiation of radiation therapy.

Original languageEnglish
JournalInternational Journal of Radiation Oncology Biology Physics
Volume84
Issue number5
DOIs
Publication statusPublished - 01-12-2012

Fingerprint

Double-Stranded DNA Breaks
Head and Neck Neoplasms
strands
radiation therapy
Radiotherapy
deoxyribonucleic acid
cancer
Radiation
radiation
Skin
Stomatitis
Mucositis
Radiation Oncology
elution
Comet Assay
lymphocytes
comets
toxicity
therapy
induction

All Science Journal Classification (ASJC) codes

  • Radiation
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

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title = "DNA double-strand break analysis by γ-H2AX foci: A useful method for determining the overreactors to radiation-induced acute reactions among head-and-neck cancer patients",
abstract = "Purpose: Interindividual variability in normal tissue toxicity during radiation therapy is a limiting factor for successful treatment. Predicting the risk of developing acute reactions before initiation of radiation therapy may have the benefit of opting for altered radiation therapy regimens to achieve minimal adverse effects with improved tumor cure. Methods and Materials: DNA double-strand break (DSB) induction and its repair kinetics in lymphocytes of head-and-neck cancer patients undergoing chemoradiation therapy was analyzed by counting γ-H2AX foci, neutral comet assay, and a modified version of neutral filter elution assay. Acute normal tissue reactions were assessed by Radiation Therapy Oncology Group criteria. Results: The correlation between residual DSBs and the severity of acute reactions demonstrated that residual γ-H2AX foci in head-and-neck cancer patients increased with the severity of oral mucositis and skin reaction. Conclusions: Our results suggest that γ-H2AX analysis may have predictive implications for identifying the overreactors to mucositis and skin reactions among head-and-neck cancer patients prior to initiation of radiation therapy.",
author = "Goutham, {Hassan Venkatesh} and Mumbrekar, {Kamalesh Dattaram} and Vadhiraja, {Bejadi Manjunath} and Fernandes, {Donald Jerard} and Krishna Sharan and {Kanive Parashiva}, Guruprasad and Satyamoorthy Kapaettu and {Bola Sadashiva}, {Satish Rao}",
year = "2012",
month = "12",
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doi = "10.1016/j.ijrobp.2012.06.041",
language = "English",
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journal = "International Journal of Radiation Oncology Biology Physics",
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T1 - DNA double-strand break analysis by γ-H2AX foci

T2 - A useful method for determining the overreactors to radiation-induced acute reactions among head-and-neck cancer patients

AU - Goutham, Hassan Venkatesh

AU - Mumbrekar, Kamalesh Dattaram

AU - Vadhiraja, Bejadi Manjunath

AU - Fernandes, Donald Jerard

AU - Sharan, Krishna

AU - Kanive Parashiva, Guruprasad

AU - Kapaettu, Satyamoorthy

AU - Bola Sadashiva, Satish Rao

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Purpose: Interindividual variability in normal tissue toxicity during radiation therapy is a limiting factor for successful treatment. Predicting the risk of developing acute reactions before initiation of radiation therapy may have the benefit of opting for altered radiation therapy regimens to achieve minimal adverse effects with improved tumor cure. Methods and Materials: DNA double-strand break (DSB) induction and its repair kinetics in lymphocytes of head-and-neck cancer patients undergoing chemoradiation therapy was analyzed by counting γ-H2AX foci, neutral comet assay, and a modified version of neutral filter elution assay. Acute normal tissue reactions were assessed by Radiation Therapy Oncology Group criteria. Results: The correlation between residual DSBs and the severity of acute reactions demonstrated that residual γ-H2AX foci in head-and-neck cancer patients increased with the severity of oral mucositis and skin reaction. Conclusions: Our results suggest that γ-H2AX analysis may have predictive implications for identifying the overreactors to mucositis and skin reactions among head-and-neck cancer patients prior to initiation of radiation therapy.

AB - Purpose: Interindividual variability in normal tissue toxicity during radiation therapy is a limiting factor for successful treatment. Predicting the risk of developing acute reactions before initiation of radiation therapy may have the benefit of opting for altered radiation therapy regimens to achieve minimal adverse effects with improved tumor cure. Methods and Materials: DNA double-strand break (DSB) induction and its repair kinetics in lymphocytes of head-and-neck cancer patients undergoing chemoradiation therapy was analyzed by counting γ-H2AX foci, neutral comet assay, and a modified version of neutral filter elution assay. Acute normal tissue reactions were assessed by Radiation Therapy Oncology Group criteria. Results: The correlation between residual DSBs and the severity of acute reactions demonstrated that residual γ-H2AX foci in head-and-neck cancer patients increased with the severity of oral mucositis and skin reaction. Conclusions: Our results suggest that γ-H2AX analysis may have predictive implications for identifying the overreactors to mucositis and skin reactions among head-and-neck cancer patients prior to initiation of radiation therapy.

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