Dosage and passage dependent neuroprotective effects of exosomes derived from rat bone marrow mesenchymal stem cells: An in vitro analysis

Chaitra Venugopal, Christopher Shamir, Sivapriya Senthilkumar, Janitri Venkatachala Babu, Peedikayil Kurien Sonu, Kusum Jain Nishtha, Kiranmai S. Rai, K. Shobha, Anandh Dhanushkodi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

ackground: Neurodegenerative diseases comprise a group of disorders for which no treatment is available till date. Stem cell based therapy offers great hope and promise. However, stem cell transplantation is associated with certain disadvantages like poor targeted migration, engraftment and survival of the transplanted cells. Material & Method: Exosomes, a type of extracellular membrane vesicle released by all cell types including stem cells, offer an alternative to stem cell transplantation. Exosome carry a wide array of biomolecules and are implicated in exhibiting substantial benefits in the repair/regeneration of the injured tissue. Thus, exosomes offer an alternative therapeutic approach as a substitute of cell transplantation. In order to utilize exosomes for therapeutic purpose, it is essential to evaluate the appropriate passage number and the dosage to avoid possible cytotoxic effects. Here, we isolated exosomes from different passages of rat bone marrow mesenchymal stem cells (BM-MSC) and analysed the neuroprotective potential of BM-MSC exosomes in an in vitro model of excitotoxicity. Result: Our results demonstrated that the exosomes isolated from early passage of rat BM-MSC exhibited more efficient neuroprotective potential as opposed to later passages derived exosomes. Furthermore, the neuroprotective efficacy of exosome is dosage dependent. i.e. the lower dosage of exosomes was found to be neuroprotective, whereas higher dosage of exosomes (from later passages) was found to be detrimental to neurons. The early passage derived exosomes protected neurons through anti-apoptotic, anti-necrotic and anti-oxidant mechanisms. Conclusion: Our study suggests that adult stem cells derived exosomes could be a potential therapeutic agent to confer neuroprotection in neurodegenerative diseases like Alzheimer’s disease.

Original languageEnglish
Pages (from-to)379-390
Number of pages12
JournalCurrent Gene Therapy
Volume17
Issue number5
DOIs
Publication statusPublished - 01-10-2017

Fingerprint

Exosomes
Neuroprotective Agents
Mesenchymal Stromal Cells
Bone Marrow
Stem Cell Transplantation
Neurodegenerative Diseases
In Vitro Techniques
Stem Cells
Neurons
Adult Stem Cells
Cell Transplantation
Therapeutics
Cell- and Tissue-Based Therapy
Oxidants

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Drug Discovery
  • Genetics(clinical)

Cite this

Venugopal, Chaitra ; Shamir, Christopher ; Senthilkumar, Sivapriya ; Babu, Janitri Venkatachala ; Sonu, Peedikayil Kurien ; Nishtha, Kusum Jain ; Rai, Kiranmai S. ; Shobha, K. ; Dhanushkodi, Anandh. / Dosage and passage dependent neuroprotective effects of exosomes derived from rat bone marrow mesenchymal stem cells : An in vitro analysis. In: Current Gene Therapy. 2017 ; Vol. 17, No. 5. pp. 379-390.
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Dosage and passage dependent neuroprotective effects of exosomes derived from rat bone marrow mesenchymal stem cells : An in vitro analysis. / Venugopal, Chaitra; Shamir, Christopher; Senthilkumar, Sivapriya; Babu, Janitri Venkatachala; Sonu, Peedikayil Kurien; Nishtha, Kusum Jain; Rai, Kiranmai S.; Shobha, K.; Dhanushkodi, Anandh.

In: Current Gene Therapy, Vol. 17, No. 5, 01.10.2017, p. 379-390.

Research output: Contribution to journalArticle

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T1 - Dosage and passage dependent neuroprotective effects of exosomes derived from rat bone marrow mesenchymal stem cells

T2 - An in vitro analysis

AU - Venugopal, Chaitra

AU - Shamir, Christopher

AU - Senthilkumar, Sivapriya

AU - Babu, Janitri Venkatachala

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AU - Nishtha, Kusum Jain

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AU - Shobha, K.

AU - Dhanushkodi, Anandh

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