Abstract

Objective: The present study was carried out to assess the effects of fenugreek seed extract on various biochemical and haematological parameters in high-fat diet (HFD)-fed rats. Methods: Female Wistar rats were allocated into five groups (n = 6): 1) control rats, 2) HFD-fed control rats 3) rats fed with HFD and fenugreek (FG) seed extract at doses of 200 mg/kg/day, 300 mg/kg/day, and 400 mg/kg/day for 12 weeks. Blood was collected to examine the biochemical and haematological parameters using a veterinary blood cell counter; blood indices such as MCV, MCH, MCHC, red blood cell distribution width, haemoglobin (Hb) levels, haematocrit, and platelet counts were measured. Blood samples were centrifuged at 3000 rpm for 10 min at room temperature to obtain serum for the estimation of lipid profiles, and aspartate transaminase and alanine transaminase levels. Results: Rats fed with FG at a dose of 400 mg/kg/day showed a significant increase in the red blood cell count, Hb levels, haematocrit, and MCV, and a significant decrease in the lymphocyte count. The total cholesterol, triglyceride, and low-density lipoprotein levels increased significantly (p < 0.05) in rats from the HFD control group, compared to those in the normal control group, but decreased significantly in rats fed with 400 mg/kg/day of FG. Conclusion: The results of the current study suggest that FG seed extract exhibits hypolipidaemic activity and significantly improves the activity of hepatic enzymes, and the blood counts and indices in rats with HFD-induced obesity.

Original languageEnglish
Pages (from-to)383-389
Number of pages7
JournalJournal of Taibah University Medical Sciences
Volume14
Issue number4
DOIs
Publication statusPublished - 01-08-2019

Fingerprint

Trigonella
High Fat Diet
Seeds
Hematocrit
Hemoglobins
Control Groups
Erythrocyte Count
Erythrocyte Indices
Lymphocyte Count
Aspartate Aminotransferases
Alanine Transaminase
Platelet Count
LDL Cholesterol
Wistar Rats
Blood Cells
Obesity
Erythrocytes
Lipids
Temperature
Liver

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{1ec7bf292fad4128b3585d7541ac928d,
title = "Dose-dependent effects of fenugreek seed extract on the biochemical and haematological parameters in high-fat diet-fed rats",
abstract = "Objective: The present study was carried out to assess the effects of fenugreek seed extract on various biochemical and haematological parameters in high-fat diet (HFD)-fed rats. Methods: Female Wistar rats were allocated into five groups (n = 6): 1) control rats, 2) HFD-fed control rats 3) rats fed with HFD and fenugreek (FG) seed extract at doses of 200 mg/kg/day, 300 mg/kg/day, and 400 mg/kg/day for 12 weeks. Blood was collected to examine the biochemical and haematological parameters using a veterinary blood cell counter; blood indices such as MCV, MCH, MCHC, red blood cell distribution width, haemoglobin (Hb) levels, haematocrit, and platelet counts were measured. Blood samples were centrifuged at 3000 rpm for 10 min at room temperature to obtain serum for the estimation of lipid profiles, and aspartate transaminase and alanine transaminase levels. Results: Rats fed with FG at a dose of 400 mg/kg/day showed a significant increase in the red blood cell count, Hb levels, haematocrit, and MCV, and a significant decrease in the lymphocyte count. The total cholesterol, triglyceride, and low-density lipoprotein levels increased significantly (p < 0.05) in rats from the HFD control group, compared to those in the normal control group, but decreased significantly in rats fed with 400 mg/kg/day of FG. Conclusion: The results of the current study suggest that FG seed extract exhibits hypolipidaemic activity and significantly improves the activity of hepatic enzymes, and the blood counts and indices in rats with HFD-induced obesity.",
author = "Takkella Nagamma and Anjaneyulu Konuri and Nayak, {Chandrika D.} and Kamath, {Shobha U.} and Udupa, {Padmanabha E.G.} and Yogendra Nayak",
year = "2019",
month = "8",
day = "1",
doi = "10.1016/j.jtumed.2019.05.003",
language = "English",
volume = "14",
pages = "383--389",
journal = "Journal of Taibah University Medical Sciences",
issn = "1658-3612",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - Dose-dependent effects of fenugreek seed extract on the biochemical and haematological parameters in high-fat diet-fed rats

AU - Nagamma, Takkella

AU - Konuri, Anjaneyulu

AU - Nayak, Chandrika D.

AU - Kamath, Shobha U.

AU - Udupa, Padmanabha E.G.

AU - Nayak, Yogendra

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Objective: The present study was carried out to assess the effects of fenugreek seed extract on various biochemical and haematological parameters in high-fat diet (HFD)-fed rats. Methods: Female Wistar rats were allocated into five groups (n = 6): 1) control rats, 2) HFD-fed control rats 3) rats fed with HFD and fenugreek (FG) seed extract at doses of 200 mg/kg/day, 300 mg/kg/day, and 400 mg/kg/day for 12 weeks. Blood was collected to examine the biochemical and haematological parameters using a veterinary blood cell counter; blood indices such as MCV, MCH, MCHC, red blood cell distribution width, haemoglobin (Hb) levels, haematocrit, and platelet counts were measured. Blood samples were centrifuged at 3000 rpm for 10 min at room temperature to obtain serum for the estimation of lipid profiles, and aspartate transaminase and alanine transaminase levels. Results: Rats fed with FG at a dose of 400 mg/kg/day showed a significant increase in the red blood cell count, Hb levels, haematocrit, and MCV, and a significant decrease in the lymphocyte count. The total cholesterol, triglyceride, and low-density lipoprotein levels increased significantly (p < 0.05) in rats from the HFD control group, compared to those in the normal control group, but decreased significantly in rats fed with 400 mg/kg/day of FG. Conclusion: The results of the current study suggest that FG seed extract exhibits hypolipidaemic activity and significantly improves the activity of hepatic enzymes, and the blood counts and indices in rats with HFD-induced obesity.

AB - Objective: The present study was carried out to assess the effects of fenugreek seed extract on various biochemical and haematological parameters in high-fat diet (HFD)-fed rats. Methods: Female Wistar rats were allocated into five groups (n = 6): 1) control rats, 2) HFD-fed control rats 3) rats fed with HFD and fenugreek (FG) seed extract at doses of 200 mg/kg/day, 300 mg/kg/day, and 400 mg/kg/day for 12 weeks. Blood was collected to examine the biochemical and haematological parameters using a veterinary blood cell counter; blood indices such as MCV, MCH, MCHC, red blood cell distribution width, haemoglobin (Hb) levels, haematocrit, and platelet counts were measured. Blood samples were centrifuged at 3000 rpm for 10 min at room temperature to obtain serum for the estimation of lipid profiles, and aspartate transaminase and alanine transaminase levels. Results: Rats fed with FG at a dose of 400 mg/kg/day showed a significant increase in the red blood cell count, Hb levels, haematocrit, and MCV, and a significant decrease in the lymphocyte count. The total cholesterol, triglyceride, and low-density lipoprotein levels increased significantly (p < 0.05) in rats from the HFD control group, compared to those in the normal control group, but decreased significantly in rats fed with 400 mg/kg/day of FG. Conclusion: The results of the current study suggest that FG seed extract exhibits hypolipidaemic activity and significantly improves the activity of hepatic enzymes, and the blood counts and indices in rats with HFD-induced obesity.

UR - http://www.scopus.com/inward/record.url?scp=85069732753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85069732753&partnerID=8YFLogxK

U2 - 10.1016/j.jtumed.2019.05.003

DO - 10.1016/j.jtumed.2019.05.003

M3 - Article

VL - 14

SP - 383

EP - 389

JO - Journal of Taibah University Medical Sciences

JF - Journal of Taibah University Medical Sciences

SN - 1658-3612

IS - 4

ER -