Drug resistance & virulence determinants in clinical isolates of Enterococcus species

Sanal C Fernandes, B Dhanashree

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

BACKGROUND & OBJECTIVES: Enterococci are the leading cause of nosocomial infections, and are thus a persisting clinical problem globally. We undertook this study to determine the virulence factors and the antibiotic resistance in Enterococcus clinical isolates.

METHODS: One hundred and fifty Enterococcus isolates obtained from various clinical specimens were speciated biochemically and subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method. Resistance to vancomycin was determined by using agar screen method. Haemolysin and gelatinase productions were detected using 5 per cent sheep blood agar and 12 per cent gelatin agar, respectively.

RESULTS: Among the 150 Enterococcus isolates, 84 (56%) were E. faecalis. 51(34%) E. faecium, and 15 (10%) were other Enterococcus spp. Haemolysin production was seen among 123 (82%) isolates while 61 (40.6%) isolates produced gelatinase. Nearly 50 per cent of the isolates showed high level aminoglycoside resistance (HLAR). A total of 13 (8.6%) isolates showed vancomycin resistance, of which 11(7.3%) had an MIC >8 μg/ml.

INTERPRETATION & CONCLUSIONS: Presence of VRE was found to be low among the isolates studied. However, occurrence of VRE along with HLAR calls for regular detection of vancomycin resistance promptly and accurately to recognize VRE colonization and infection. Early detection of VRE and HLAR along with their virulence trait will help in preventing the establishment and spread of multidrug resistant Enterococcus species.

Original languageEnglish
Pages (from-to)981-5
Number of pages5
JournalIndian Journal of Medical Research
Volume137
Issue number5
Publication statusPublished - 05-2013

Fingerprint

Enterococcus
Aminoglycosides
Vancomycin
Drug Resistance
Agar
Virulence
Gelatinases
Hemolysin Proteins
Vancomycin Resistance
Pharmaceutical Preparations
Anti-Bacterial Agents
Virulence Factors
Gelatin
Blood
Disk Diffusion Antimicrobial Tests
Testing
Microbial Drug Resistance
Cross Infection
Sheep
Infection

Cite this

@article{55e2bd6cd0f04e2bae96a4b286cf0c39,
title = "Drug resistance & virulence determinants in clinical isolates of Enterococcus species",
abstract = "BACKGROUND & OBJECTIVES: Enterococci are the leading cause of nosocomial infections, and are thus a persisting clinical problem globally. We undertook this study to determine the virulence factors and the antibiotic resistance in Enterococcus clinical isolates.METHODS: One hundred and fifty Enterococcus isolates obtained from various clinical specimens were speciated biochemically and subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method. Resistance to vancomycin was determined by using agar screen method. Haemolysin and gelatinase productions were detected using 5 per cent sheep blood agar and 12 per cent gelatin agar, respectively.RESULTS: Among the 150 Enterococcus isolates, 84 (56{\%}) were E. faecalis. 51(34{\%}) E. faecium, and 15 (10{\%}) were other Enterococcus spp. Haemolysin production was seen among 123 (82{\%}) isolates while 61 (40.6{\%}) isolates produced gelatinase. Nearly 50 per cent of the isolates showed high level aminoglycoside resistance (HLAR). A total of 13 (8.6{\%}) isolates showed vancomycin resistance, of which 11(7.3{\%}) had an MIC >8 μg/ml.INTERPRETATION & CONCLUSIONS: Presence of VRE was found to be low among the isolates studied. However, occurrence of VRE along with HLAR calls for regular detection of vancomycin resistance promptly and accurately to recognize VRE colonization and infection. Early detection of VRE and HLAR along with their virulence trait will help in preventing the establishment and spread of multidrug resistant Enterococcus species.",
author = "Fernandes, {Sanal C} and B Dhanashree",
year = "2013",
month = "5",
language = "English",
volume = "137",
pages = "981--5",
journal = "Indian Journal of Medical Research",
issn = "0971-5916",
publisher = "Indian Council of Medical Research",
number = "5",

}

Drug resistance & virulence determinants in clinical isolates of Enterococcus species. / Fernandes, Sanal C; Dhanashree, B.

In: Indian Journal of Medical Research, Vol. 137, No. 5, 05.2013, p. 981-5.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Drug resistance & virulence determinants in clinical isolates of Enterococcus species

AU - Fernandes, Sanal C

AU - Dhanashree, B

PY - 2013/5

Y1 - 2013/5

N2 - BACKGROUND & OBJECTIVES: Enterococci are the leading cause of nosocomial infections, and are thus a persisting clinical problem globally. We undertook this study to determine the virulence factors and the antibiotic resistance in Enterococcus clinical isolates.METHODS: One hundred and fifty Enterococcus isolates obtained from various clinical specimens were speciated biochemically and subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method. Resistance to vancomycin was determined by using agar screen method. Haemolysin and gelatinase productions were detected using 5 per cent sheep blood agar and 12 per cent gelatin agar, respectively.RESULTS: Among the 150 Enterococcus isolates, 84 (56%) were E. faecalis. 51(34%) E. faecium, and 15 (10%) were other Enterococcus spp. Haemolysin production was seen among 123 (82%) isolates while 61 (40.6%) isolates produced gelatinase. Nearly 50 per cent of the isolates showed high level aminoglycoside resistance (HLAR). A total of 13 (8.6%) isolates showed vancomycin resistance, of which 11(7.3%) had an MIC >8 μg/ml.INTERPRETATION & CONCLUSIONS: Presence of VRE was found to be low among the isolates studied. However, occurrence of VRE along with HLAR calls for regular detection of vancomycin resistance promptly and accurately to recognize VRE colonization and infection. Early detection of VRE and HLAR along with their virulence trait will help in preventing the establishment and spread of multidrug resistant Enterococcus species.

AB - BACKGROUND & OBJECTIVES: Enterococci are the leading cause of nosocomial infections, and are thus a persisting clinical problem globally. We undertook this study to determine the virulence factors and the antibiotic resistance in Enterococcus clinical isolates.METHODS: One hundred and fifty Enterococcus isolates obtained from various clinical specimens were speciated biochemically and subjected to antibiotic susceptibility testing using Kirby-Bauer disk diffusion method. Resistance to vancomycin was determined by using agar screen method. Haemolysin and gelatinase productions were detected using 5 per cent sheep blood agar and 12 per cent gelatin agar, respectively.RESULTS: Among the 150 Enterococcus isolates, 84 (56%) were E. faecalis. 51(34%) E. faecium, and 15 (10%) were other Enterococcus spp. Haemolysin production was seen among 123 (82%) isolates while 61 (40.6%) isolates produced gelatinase. Nearly 50 per cent of the isolates showed high level aminoglycoside resistance (HLAR). A total of 13 (8.6%) isolates showed vancomycin resistance, of which 11(7.3%) had an MIC >8 μg/ml.INTERPRETATION & CONCLUSIONS: Presence of VRE was found to be low among the isolates studied. However, occurrence of VRE along with HLAR calls for regular detection of vancomycin resistance promptly and accurately to recognize VRE colonization and infection. Early detection of VRE and HLAR along with their virulence trait will help in preventing the establishment and spread of multidrug resistant Enterococcus species.

M3 - Article

C2 - 23760387

VL - 137

SP - 981

EP - 985

JO - Indian Journal of Medical Research

JF - Indian Journal of Medical Research

SN - 0971-5916

IS - 5

ER -