Drug susceptibility profiles of Mycobacterium tuberculosis isolates at Mangalore

Sevitha Bhat, M. Radhakrishna, Mohan Kotian, Sunil Rao

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background and Objectives: Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. Its resurgence in 1990s is primarily due to co-infection with HIV and the emergence of multi-drug-resistant (MDR) strains. Our objectives in this study were demonstration and grading of acid-fast bacilli in smears from sputum specimens of clinically newly diagnosed pulmonary TB patients, isolation of the organism, speciation and drug susceptibility testing of Mycobacterium tuberculosis isolates to isoniazid (H), rifampicin (R), streptomycin (S), and ethambutol (E). Materials and Methods: Sputum specimens were collected from 150 patients. Smear examination was done after Ziehl-Neelsen staining. The specimens were cultured onto Lowenstein Jensen media after Petroff′s method of concentration. The growth was identified as M. tuberculosis with standard tests. Sensitivity of 50 isolates of tubercle bacilli to anti-TB drugs H, R, S, E were determined by Resistance-Ratio method. Results: Out of 150 sputum specimens examined, 62(41.3%) were smear positive. Out of these 62,56 grew on culture. 50 isolates of M. tuberculosis were picked up for drug susceptibility testing. Total of 31 (62%) were resistant to S, 14(28%) to H, 9(18%) to R, 6(12%) were resistant to E and 2 strains (4%) were resistant to H and R. Conclusion: From the small cohort, incidence of primary MDR-TB was found to be 4% in this region, which is within the expected range.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalIndian Journal of Medical Sciences
Volume64
Issue number3
DOIs
Publication statusPublished - 01-03-2010

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Mycobacterium tuberculosis
Sputum
Pharmaceutical Preparations
Bacillus
Tuberculosis
Patient Isolation
Ethambutol
Multidrug-Resistant Tuberculosis
Isoniazid
Streptomycin
Rifampin
Coinfection
Pulmonary Tuberculosis
Cause of Death
HIV
Staining and Labeling
Acids
Incidence
Growth

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Drug susceptibility profiles of Mycobacterium tuberculosis isolates at Mangalore",
abstract = "Background and Objectives: Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. Its resurgence in 1990s is primarily due to co-infection with HIV and the emergence of multi-drug-resistant (MDR) strains. Our objectives in this study were demonstration and grading of acid-fast bacilli in smears from sputum specimens of clinically newly diagnosed pulmonary TB patients, isolation of the organism, speciation and drug susceptibility testing of Mycobacterium tuberculosis isolates to isoniazid (H), rifampicin (R), streptomycin (S), and ethambutol (E). Materials and Methods: Sputum specimens were collected from 150 patients. Smear examination was done after Ziehl-Neelsen staining. The specimens were cultured onto Lowenstein Jensen media after Petroff′s method of concentration. The growth was identified as M. tuberculosis with standard tests. Sensitivity of 50 isolates of tubercle bacilli to anti-TB drugs H, R, S, E were determined by Resistance-Ratio method. Results: Out of 150 sputum specimens examined, 62(41.3{\%}) were smear positive. Out of these 62,56 grew on culture. 50 isolates of M. tuberculosis were picked up for drug susceptibility testing. Total of 31 (62{\%}) were resistant to S, 14(28{\%}) to H, 9(18{\%}) to R, 6(12{\%}) were resistant to E and 2 strains (4{\%}) were resistant to H and R. Conclusion: From the small cohort, incidence of primary MDR-TB was found to be 4{\%} in this region, which is within the expected range.",
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Drug susceptibility profiles of Mycobacterium tuberculosis isolates at Mangalore. / Bhat, Sevitha; Radhakrishna, M.; Kotian, Mohan; Rao, Sunil.

In: Indian Journal of Medical Sciences, Vol. 64, No. 3, 01.03.2010, p. 99-103.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Drug susceptibility profiles of Mycobacterium tuberculosis isolates at Mangalore

AU - Bhat, Sevitha

AU - Radhakrishna, M.

AU - Kotian, Mohan

AU - Rao, Sunil

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AB - Background and Objectives: Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. Its resurgence in 1990s is primarily due to co-infection with HIV and the emergence of multi-drug-resistant (MDR) strains. Our objectives in this study were demonstration and grading of acid-fast bacilli in smears from sputum specimens of clinically newly diagnosed pulmonary TB patients, isolation of the organism, speciation and drug susceptibility testing of Mycobacterium tuberculosis isolates to isoniazid (H), rifampicin (R), streptomycin (S), and ethambutol (E). Materials and Methods: Sputum specimens were collected from 150 patients. Smear examination was done after Ziehl-Neelsen staining. The specimens were cultured onto Lowenstein Jensen media after Petroff′s method of concentration. The growth was identified as M. tuberculosis with standard tests. Sensitivity of 50 isolates of tubercle bacilli to anti-TB drugs H, R, S, E were determined by Resistance-Ratio method. Results: Out of 150 sputum specimens examined, 62(41.3%) were smear positive. Out of these 62,56 grew on culture. 50 isolates of M. tuberculosis were picked up for drug susceptibility testing. Total of 31 (62%) were resistant to S, 14(28%) to H, 9(18%) to R, 6(12%) were resistant to E and 2 strains (4%) were resistant to H and R. Conclusion: From the small cohort, incidence of primary MDR-TB was found to be 4% in this region, which is within the expected range.

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