Effect of 5-aminosalicylic acid on radiation-induced micronuclei in mouse bone marrow

M. Sudheer Kumar, M.K. Unnikrishnan, P. Uma Devi

Research output: Contribution to journalArticle

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Abstract

5-Aminosalicylic acid (5ASA), a prescribed drug for ulcerative colitis, is a potent scavenger of oxygen-derived free radicals. The present study was undertaken to ascertain its ability to protect against radiation-induced damage. The drug dose-dependent effect, optimum time of drug administration and radiation dose-dependent effect (0-4Gy) on in vivo radiation protection against micronuclei induction in polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) were studied in the bone marrow of mice. Intraperitoneal injection of 10-125mg/kg of the drug 30min before whole body irradiation with 3Gy produced a significant reduction in the frequency of micronucleated erythrocytes at 24h after exposure. The optimum dose for protection without drug toxicity was 25mg/kg body weight. Injection of 25mg/kg of the drug 60 or 30min before or within 15min after 3Gy whole body γ-irradiation resulted in a significant decrease in the radiation-induced PCE and NCE with micronuclei (MPCE and MNCE) and an increase in the ratio of PCE to NCE (P/N), at 24h post-irradiation. Maximum effect was seen when the drug was administered 30min before irradiation. Therefore, to study the radiation dose-response, mice were pre-treated with 25mg/kg of 5ASA 30min before 1-4Gy of γ-irradiation. Radiation increased the MN frequency linearly (r2=0.99) with dose. Pre-treatment with 5ASA significantly reduced the MN counts to 40-50% of the radiation (RT) alone values, giving a dose modification factor (DMF) of 2.02 (MPCE) and 2.53 (MNCE). Irradiation resulted in a dose-dependent decline in the P/N ratio at all the doses of radiation studied. 5ASA produced a significant increase in the P/N ratio from that of irradiated controls, at all doses of radiations tested. These results show that 5ASA protect mice against radiation-induced MN formation and mitotic arrest. © 2003 Elsevier Science B.V. All rights reserved.
Original languageEnglish
Pages (from-to)7-14
Number of pages8
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume527
Issue number1-2
DOIs
Publication statusPublished - 2003

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Mesalamine
Bone Marrow
Radiation
Erythrocytes
Pharmaceutical Preparations
Whole-Body Irradiation
Radiation Protection
Intraperitoneal Injections
Drug-Related Side Effects and Adverse Reactions
Ulcerative Colitis
Free Radicals
Body Weight
Oxygen
Injections

Cite this

@article{fc00997242764f43a84594ab07426bda,
title = "Effect of 5-aminosalicylic acid on radiation-induced micronuclei in mouse bone marrow",
abstract = "5-Aminosalicylic acid (5ASA), a prescribed drug for ulcerative colitis, is a potent scavenger of oxygen-derived free radicals. The present study was undertaken to ascertain its ability to protect against radiation-induced damage. The drug dose-dependent effect, optimum time of drug administration and radiation dose-dependent effect (0-4Gy) on in vivo radiation protection against micronuclei induction in polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) were studied in the bone marrow of mice. Intraperitoneal injection of 10-125mg/kg of the drug 30min before whole body irradiation with 3Gy produced a significant reduction in the frequency of micronucleated erythrocytes at 24h after exposure. The optimum dose for protection without drug toxicity was 25mg/kg body weight. Injection of 25mg/kg of the drug 60 or 30min before or within 15min after 3Gy whole body γ-irradiation resulted in a significant decrease in the radiation-induced PCE and NCE with micronuclei (MPCE and MNCE) and an increase in the ratio of PCE to NCE (P/N), at 24h post-irradiation. Maximum effect was seen when the drug was administered 30min before irradiation. Therefore, to study the radiation dose-response, mice were pre-treated with 25mg/kg of 5ASA 30min before 1-4Gy of γ-irradiation. Radiation increased the MN frequency linearly (r2=0.99) with dose. Pre-treatment with 5ASA significantly reduced the MN counts to 40-50{\%} of the radiation (RT) alone values, giving a dose modification factor (DMF) of 2.02 (MPCE) and 2.53 (MNCE). Irradiation resulted in a dose-dependent decline in the P/N ratio at all the doses of radiation studied. 5ASA produced a significant increase in the P/N ratio from that of irradiated controls, at all doses of radiations tested. These results show that 5ASA protect mice against radiation-induced MN formation and mitotic arrest. {\circledC} 2003 Elsevier Science B.V. All rights reserved.",
author = "{Sudheer Kumar}, M. and M.K. Unnikrishnan and {Uma Devi}, P.",
note = "Cited By :24 Export Date: 10 November 2017 CODEN: MRFME Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, College of Pharmaceutical Sciences, Manipal 576119, India; email: Cheruvaloor@yahoo.com Chemicals/CAS: mesalazine, 89-57-6 Manufacturers: Sigma, United States References: Uma Devi, P., Normal tissue protection in cancer therapy: Progress and prospects (1998) Acta Oncol., 37, pp. 247-252; Kemp, G., Rose, P., Lurain, J., Amifostine pre-treatment for protection against cyclophosphamide-induced and cisplatin-induced toxicities: Results of a randomised control trail in patients with advanced ovarian cancer (1996) J. Clin. Oncol., 14, p. 2101; Foster-Nora, J.A., Siden, R., Amifostine for protection from antineoplastic drug toxicity (1997) Am. J. Health Syst. Pharm., 54, p. 787; Miles, A.M., Grisham, M.B., Antioxidant properties of aminosalicylates (1994) Methods Enzymol., 234, pp. 555-572; Brogden, R.N., Sorkin, E.M., Mesalazine: A view of its pharmacodynamic and pharmacokinetic properties, and therapeutic potential in chronic inflammatory bowel disease (1989) Drugs, 38, pp. 500-523; Del Soldato, P., Campieri, M., Brignola, C., Bazzocchi, G., Gionchetti, P., Lanfranchi, G.A., Tamba, M., A possible mechanism of action of sulfasalazine and 5-aminosalicylic acid in inflammatory bowel diseases: Interaction with oxygen free radicals (1985) Gastroenterology, 89, pp. 1215-1216; Satyamitra, M., Uma Devi, P., Murase, H., Tsutoma Kagiya, V., In vivo radioprotection by α-TMG: Preliminary studies (2001) Mutat. Res., 479, pp. 53-61; Fischer-Nielsen, A., Poulsen, H.E., Loft, S., 8-Hydroxydeoxyguanosine in vitro: Effects of glutathione, ascorbate and 5-aminosalicylic acid (1992) Free Radic. Biol. Med., 13, pp. 121-126; Fischer-Nielsen, A., Jeding, I.B., Loft, S., Radiation-induced formation of 8-hydroxy-2′-deoxyguanosine and its prevention by scavengers (1994) Carcinogenesis, 15, pp. 1609-1612; Fischer-Nielsen, A., Loft, S., Jensen, K.G., Effect of ascorbate and 5-aminosalicylic acid on light-induced 8-hydroxydeoxyguanosine formation in V79 hamster cells (1993) Carcinogenesis, 14, pp. 2431-2433; Uma Devi, P., Ganasoundari, A., Rao, B.S.S., Srinivasan, K.K., In vivo radioprotection by Ocimum flavonoids: Survival of mice (1999) Radiat. Res., 151, pp. 74-78; Ghosh, M.N., Toxicity studies (1984) Fundamentals of Experimental Pharmacology, 2nd Ed., pp. 153-157. , Scientific Book Agency, Calcutta; Geigy, J.R., Statistical methods in medicine (1956) Documents Geigy Scientific Tables, 5th Ed., pp. 26-48. , J. Board & Co. Ltd., UK; Schmid, W., The micronucleus test (1975) Mutat. Res., 31, pp. 9-15; Brusick, D., Principles of Genetic Toxicology, , Plenum Press, New York; Hofer, M., Mazur, L., Pospil, M., Weiterova, L., Znojil, V., Radioprotective action of extracellular adenosine on bone marrow cells in mice exposed to gamma rays as assayed by micronucleus test (2000) Radiat. Res., 154, pp. 217-221; Jagetia, G.C., Ganapathi, N.G., Effect of copperglycinate on the radiation-induced micronuclei formation in mice bonemarrow (1990) Radiat. Environ. Biophys., 29, pp. 115-118; Uma Devi, P., Sharma, A.S.K.V.S., Mouse bonemarrow response to low doses of whole-body gamma irradiation: Induction of micronuclei (1990) Int. J. Radiat. Biol., 57, pp. 97-101; Jagetia, G.C., Aruna, R., The herbal preparation abana protects against radiation-induced micronuclei in mouse bone marrow (1997) Mutat. Res., 393, pp. 157-163; Chaubey, R.C., Bhilwade, H.N., N Joshi, B., Chauhan, P.S., Studies on the migration of micronucleated erythrocytes from bone marrow to the peripheral blood in irradiated Swiss mice (1993) Int. J. Radiat. Biol., 63, pp. 239-245; Uma Devi, P., Ganasoundari, A., Radioprotective effect of leaf extract of Indian medicinal plant Ocimum sanctum (1995) Ind. J. Exp. Biol., 33, pp. 205-208; Grzelinska, E., Bartkowiak, A., Bartosz, G., Leyko, W., Effect of ·OH scavengers on radiation damage to the erythrocyte membrane (1982) Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med., 41, pp. 473-481; Baum, C.A., Biddle, W.L., Miner, P.B., Failure of 5-aminosalicylic acid enemas to improve radiation proctitis (1989) Dig. Dis. Sci., 34, pp. 758-760; Baugham, C.A., Canney, P.A., Buchanan, R.B., Pickering, R.M., A randomized trail to assess the efficiency of 5-aminosalicylic acid for prevention of radiation enteritis (1993) Clin. Oncol. (R. Coll. Radiol.), 5, pp. 19-24; Freund, U., Scholmerich, J., Siems, H., Kluge, F., Schafer, H.E., Wannenmachner, M., Unerwunschte Nebenwirkungen bie Anwendung von Mesalzine (5-aminosalicylic acid) under Strahlentherapie (1987) Strahlenther. Onkol., 163, pp. 678-680; Maisin, J.R., Albert, C., Henry, A., Reduction of short-term radiation lethality by biological response modifiers given alone or in association with other chemical protectors (1993) Radiat. Res., 135, pp. 332-337; Savage, J.R.K., A comment on the quantitative relationship between micronuclei and chromosomal aberrations (1998) Mutat. Res., 207, pp. 33-36; Sasaki, M.S., Matsubara, S., Free radical scavenging in protection of human lymphocytes against chromosome aberration formation by gamma-ray irradiation (1977) Int. J. Radiat. Biol., 32, pp. 439-445; Roots, R., Okada, S., Protection of DNA molecules of cultured mammalian cells from radiation-induced single-strand scissions by various alcohols and SH compounds (1972) Int. J. Radiat. Biol., 21, pp. 329-342; Okada, S., Nakamura, N., Sasaki, K., Radioprotection of intracellular genetic material (1983) Radioprotection and Anticarcinogens, pp. 339-356. , O.F. Nygaard, M.G. Simic (Eds.), Academic Press, New York; Shimoi, K., Masuda, S., Shen, B., Furugori, M., Kinae, N., Radiation protection effect of antioxidative plant flavonoids in mice (1996) Mutat. Res., 350, pp. 153-161; Uma Devi, P., Ganasoundari, A., Vrinda, B., Srinivasan, K.K., Unnikrishnan, M.K., Radiation protection by Ocimum flavonoids orientin and vicenin: Mechanism of action (2000) Radiat. Res., 154, pp. 455-460; Abraham, S.K., Sarma, L., Kesavan, P.C., Protective effects of chlorogenic acid, curcumin and β-carotene against γ-radiation-induced in vivo chromosomal damage (1993) Mutat. Res., 303, pp. 109-112; Sarma, L., Kesavan, P.C., Protective effects of Vitamin C and E against γ-ray-induced chromosomal damage in mouse (1993) Int. J. Radiat. Biol., 63, pp. 759-764; Umegaki, K., Ikegami, S., Inoue, K., Ichikawa, T., Kobayashi, S., Soeno, N., Tomebachi, K., Beta-carotene prevents X-ray induction of micronuclei in human lymphocytes (1994) Am. J. Clin. Nutr., 59, pp. 409-412; Aruoma, O.I., Wasil, M., Halliwell, B., Hoey, B.M., Buttler, J., The Scavenging of oxidants by sulfasalazine and its metabolites: A possible contribution to their anti-inflammatory effects? (1987) Biochem. Pharmacol., 36, pp. 3739-3742",
year = "2003",
doi = "10.1016/S0027-5107(03)00052-6",
language = "English",
volume = "527",
pages = "7--14",
journal = "Mutation Research",
issn = "1386-1964",
publisher = "Elsevier",
number = "1-2",

}

Effect of 5-aminosalicylic acid on radiation-induced micronuclei in mouse bone marrow. / Sudheer Kumar, M.; Unnikrishnan, M.K.; Uma Devi, P.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 527, No. 1-2, 2003, p. 7-14.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of 5-aminosalicylic acid on radiation-induced micronuclei in mouse bone marrow

AU - Sudheer Kumar, M.

AU - Unnikrishnan, M.K.

AU - Uma Devi, P.

N1 - Cited By :24 Export Date: 10 November 2017 CODEN: MRFME Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, College of Pharmaceutical Sciences, Manipal 576119, India; email: Cheruvaloor@yahoo.com Chemicals/CAS: mesalazine, 89-57-6 Manufacturers: Sigma, United States References: Uma Devi, P., Normal tissue protection in cancer therapy: Progress and prospects (1998) Acta Oncol., 37, pp. 247-252; Kemp, G., Rose, P., Lurain, J., Amifostine pre-treatment for protection against cyclophosphamide-induced and cisplatin-induced toxicities: Results of a randomised control trail in patients with advanced ovarian cancer (1996) J. Clin. Oncol., 14, p. 2101; Foster-Nora, J.A., Siden, R., Amifostine for protection from antineoplastic drug toxicity (1997) Am. J. Health Syst. Pharm., 54, p. 787; Miles, A.M., Grisham, M.B., Antioxidant properties of aminosalicylates (1994) Methods Enzymol., 234, pp. 555-572; Brogden, R.N., Sorkin, E.M., Mesalazine: A view of its pharmacodynamic and pharmacokinetic properties, and therapeutic potential in chronic inflammatory bowel disease (1989) Drugs, 38, pp. 500-523; Del Soldato, P., Campieri, M., Brignola, C., Bazzocchi, G., Gionchetti, P., Lanfranchi, G.A., Tamba, M., A possible mechanism of action of sulfasalazine and 5-aminosalicylic acid in inflammatory bowel diseases: Interaction with oxygen free radicals (1985) Gastroenterology, 89, pp. 1215-1216; Satyamitra, M., Uma Devi, P., Murase, H., Tsutoma Kagiya, V., In vivo radioprotection by α-TMG: Preliminary studies (2001) Mutat. Res., 479, pp. 53-61; Fischer-Nielsen, A., Poulsen, H.E., Loft, S., 8-Hydroxydeoxyguanosine in vitro: Effects of glutathione, ascorbate and 5-aminosalicylic acid (1992) Free Radic. Biol. Med., 13, pp. 121-126; Fischer-Nielsen, A., Jeding, I.B., Loft, S., Radiation-induced formation of 8-hydroxy-2′-deoxyguanosine and its prevention by scavengers (1994) Carcinogenesis, 15, pp. 1609-1612; Fischer-Nielsen, A., Loft, S., Jensen, K.G., Effect of ascorbate and 5-aminosalicylic acid on light-induced 8-hydroxydeoxyguanosine formation in V79 hamster cells (1993) Carcinogenesis, 14, pp. 2431-2433; Uma Devi, P., Ganasoundari, A., Rao, B.S.S., Srinivasan, K.K., In vivo radioprotection by Ocimum flavonoids: Survival of mice (1999) Radiat. Res., 151, pp. 74-78; Ghosh, M.N., Toxicity studies (1984) Fundamentals of Experimental Pharmacology, 2nd Ed., pp. 153-157. , Scientific Book Agency, Calcutta; Geigy, J.R., Statistical methods in medicine (1956) Documents Geigy Scientific Tables, 5th Ed., pp. 26-48. , J. Board & Co. Ltd., UK; Schmid, W., The micronucleus test (1975) Mutat. Res., 31, pp. 9-15; Brusick, D., Principles of Genetic Toxicology, , Plenum Press, New York; Hofer, M., Mazur, L., Pospil, M., Weiterova, L., Znojil, V., Radioprotective action of extracellular adenosine on bone marrow cells in mice exposed to gamma rays as assayed by micronucleus test (2000) Radiat. Res., 154, pp. 217-221; Jagetia, G.C., Ganapathi, N.G., Effect of copperglycinate on the radiation-induced micronuclei formation in mice bonemarrow (1990) Radiat. Environ. Biophys., 29, pp. 115-118; Uma Devi, P., Sharma, A.S.K.V.S., Mouse bonemarrow response to low doses of whole-body gamma irradiation: Induction of micronuclei (1990) Int. J. Radiat. Biol., 57, pp. 97-101; Jagetia, G.C., Aruna, R., The herbal preparation abana protects against radiation-induced micronuclei in mouse bone marrow (1997) Mutat. Res., 393, pp. 157-163; Chaubey, R.C., Bhilwade, H.N., N Joshi, B., Chauhan, P.S., Studies on the migration of micronucleated erythrocytes from bone marrow to the peripheral blood in irradiated Swiss mice (1993) Int. J. Radiat. Biol., 63, pp. 239-245; Uma Devi, P., Ganasoundari, A., Radioprotective effect of leaf extract of Indian medicinal plant Ocimum sanctum (1995) Ind. J. Exp. Biol., 33, pp. 205-208; Grzelinska, E., Bartkowiak, A., Bartosz, G., Leyko, W., Effect of ·OH scavengers on radiation damage to the erythrocyte membrane (1982) Int. J. Radiat. Biol. Relat. Stud. Phys. Chem. Med., 41, pp. 473-481; Baum, C.A., Biddle, W.L., Miner, P.B., Failure of 5-aminosalicylic acid enemas to improve radiation proctitis (1989) Dig. Dis. Sci., 34, pp. 758-760; Baugham, C.A., Canney, P.A., Buchanan, R.B., Pickering, R.M., A randomized trail to assess the efficiency of 5-aminosalicylic acid for prevention of radiation enteritis (1993) Clin. Oncol. (R. Coll. Radiol.), 5, pp. 19-24; Freund, U., Scholmerich, J., Siems, H., Kluge, F., Schafer, H.E., Wannenmachner, M., Unerwunschte Nebenwirkungen bie Anwendung von Mesalzine (5-aminosalicylic acid) under Strahlentherapie (1987) Strahlenther. Onkol., 163, pp. 678-680; Maisin, J.R., Albert, C., Henry, A., Reduction of short-term radiation lethality by biological response modifiers given alone or in association with other chemical protectors (1993) Radiat. Res., 135, pp. 332-337; Savage, J.R.K., A comment on the quantitative relationship between micronuclei and chromosomal aberrations (1998) Mutat. Res., 207, pp. 33-36; Sasaki, M.S., Matsubara, S., Free radical scavenging in protection of human lymphocytes against chromosome aberration formation by gamma-ray irradiation (1977) Int. J. Radiat. Biol., 32, pp. 439-445; Roots, R., Okada, S., Protection of DNA molecules of cultured mammalian cells from radiation-induced single-strand scissions by various alcohols and SH compounds (1972) Int. J. Radiat. Biol., 21, pp. 329-342; Okada, S., Nakamura, N., Sasaki, K., Radioprotection of intracellular genetic material (1983) Radioprotection and Anticarcinogens, pp. 339-356. , O.F. Nygaard, M.G. Simic (Eds.), Academic Press, New York; Shimoi, K., Masuda, S., Shen, B., Furugori, M., Kinae, N., Radiation protection effect of antioxidative plant flavonoids in mice (1996) Mutat. Res., 350, pp. 153-161; Uma Devi, P., Ganasoundari, A., Vrinda, B., Srinivasan, K.K., Unnikrishnan, M.K., Radiation protection by Ocimum flavonoids orientin and vicenin: Mechanism of action (2000) Radiat. Res., 154, pp. 455-460; Abraham, S.K., Sarma, L., Kesavan, P.C., Protective effects of chlorogenic acid, curcumin and β-carotene against γ-radiation-induced in vivo chromosomal damage (1993) Mutat. Res., 303, pp. 109-112; Sarma, L., Kesavan, P.C., Protective effects of Vitamin C and E against γ-ray-induced chromosomal damage in mouse (1993) Int. J. Radiat. Biol., 63, pp. 759-764; Umegaki, K., Ikegami, S., Inoue, K., Ichikawa, T., Kobayashi, S., Soeno, N., Tomebachi, K., Beta-carotene prevents X-ray induction of micronuclei in human lymphocytes (1994) Am. J. Clin. Nutr., 59, pp. 409-412; Aruoma, O.I., Wasil, M., Halliwell, B., Hoey, B.M., Buttler, J., The Scavenging of oxidants by sulfasalazine and its metabolites: A possible contribution to their anti-inflammatory effects? (1987) Biochem. Pharmacol., 36, pp. 3739-3742

PY - 2003

Y1 - 2003

N2 - 5-Aminosalicylic acid (5ASA), a prescribed drug for ulcerative colitis, is a potent scavenger of oxygen-derived free radicals. The present study was undertaken to ascertain its ability to protect against radiation-induced damage. The drug dose-dependent effect, optimum time of drug administration and radiation dose-dependent effect (0-4Gy) on in vivo radiation protection against micronuclei induction in polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) were studied in the bone marrow of mice. Intraperitoneal injection of 10-125mg/kg of the drug 30min before whole body irradiation with 3Gy produced a significant reduction in the frequency of micronucleated erythrocytes at 24h after exposure. The optimum dose for protection without drug toxicity was 25mg/kg body weight. Injection of 25mg/kg of the drug 60 or 30min before or within 15min after 3Gy whole body γ-irradiation resulted in a significant decrease in the radiation-induced PCE and NCE with micronuclei (MPCE and MNCE) and an increase in the ratio of PCE to NCE (P/N), at 24h post-irradiation. Maximum effect was seen when the drug was administered 30min before irradiation. Therefore, to study the radiation dose-response, mice were pre-treated with 25mg/kg of 5ASA 30min before 1-4Gy of γ-irradiation. Radiation increased the MN frequency linearly (r2=0.99) with dose. Pre-treatment with 5ASA significantly reduced the MN counts to 40-50% of the radiation (RT) alone values, giving a dose modification factor (DMF) of 2.02 (MPCE) and 2.53 (MNCE). Irradiation resulted in a dose-dependent decline in the P/N ratio at all the doses of radiation studied. 5ASA produced a significant increase in the P/N ratio from that of irradiated controls, at all doses of radiations tested. These results show that 5ASA protect mice against radiation-induced MN formation and mitotic arrest. © 2003 Elsevier Science B.V. All rights reserved.

AB - 5-Aminosalicylic acid (5ASA), a prescribed drug for ulcerative colitis, is a potent scavenger of oxygen-derived free radicals. The present study was undertaken to ascertain its ability to protect against radiation-induced damage. The drug dose-dependent effect, optimum time of drug administration and radiation dose-dependent effect (0-4Gy) on in vivo radiation protection against micronuclei induction in polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) were studied in the bone marrow of mice. Intraperitoneal injection of 10-125mg/kg of the drug 30min before whole body irradiation with 3Gy produced a significant reduction in the frequency of micronucleated erythrocytes at 24h after exposure. The optimum dose for protection without drug toxicity was 25mg/kg body weight. Injection of 25mg/kg of the drug 60 or 30min before or within 15min after 3Gy whole body γ-irradiation resulted in a significant decrease in the radiation-induced PCE and NCE with micronuclei (MPCE and MNCE) and an increase in the ratio of PCE to NCE (P/N), at 24h post-irradiation. Maximum effect was seen when the drug was administered 30min before irradiation. Therefore, to study the radiation dose-response, mice were pre-treated with 25mg/kg of 5ASA 30min before 1-4Gy of γ-irradiation. Radiation increased the MN frequency linearly (r2=0.99) with dose. Pre-treatment with 5ASA significantly reduced the MN counts to 40-50% of the radiation (RT) alone values, giving a dose modification factor (DMF) of 2.02 (MPCE) and 2.53 (MNCE). Irradiation resulted in a dose-dependent decline in the P/N ratio at all the doses of radiation studied. 5ASA produced a significant increase in the P/N ratio from that of irradiated controls, at all doses of radiations tested. These results show that 5ASA protect mice against radiation-induced MN formation and mitotic arrest. © 2003 Elsevier Science B.V. All rights reserved.

U2 - 10.1016/S0027-5107(03)00052-6

DO - 10.1016/S0027-5107(03)00052-6

M3 - Article

VL - 527

SP - 7

EP - 14

JO - Mutation Research

JF - Mutation Research

SN - 1386-1964

IS - 1-2

ER -